Assays

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3 Assays visible to you, out of a total of 3

Specific activity of Gre2p

Towards Reproducible Enzyme Modeling

Specific activity of Gre2p measured by following the change in absorbance of NADPH at 340 nm for the conversion of nitrononane-2,8-dione (NDK).

Submitter: Gudrun Gygli

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Workflow for characterization of enzymes under ...

Study: Spectrophotometric Activity Measurements

Organisms: No organisms

SOPs: Python Workflow for Analysis of Specific Activi..., SOP for specific activity measurements

Data files: Specific activity of Gre2p after different trea..., Specific activity of Gre2p after different trea..., Specific activity of Gre2p after incubations wi..., Specific activity of Gre2p after incubations wi...

Snapshots: No snapshots

Created: 21st Apr 2021 at 08:28, Last updated: 5th May 2021 at 12:02

Kinetic parameters of Gre2p

Towards Reproducible Enzyme Modeling

Kinetic parameters (Km, kcat) of Gre2p measured by following the change in absorbance of NADPH at 340 nm for the conversion of nitrononane-2,8-dione (NDK) or hexane-2,5-dione. Initial rates at different substrate concentrations are measured.

Submitter: Gudrun Gygli

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Workflow for characterization of enzymes under ...

Study: Spectrophotometric Activity Measurements

Organisms: No organisms

SOPs: SOP for enzyme kinetics measurements

Data files: Enzyme kinetics measurement of Gre2p at differe..., Enzyme kinetics measurement of Gre2p at differe..., Enzyme kinetics measurement of Gre2p at differe..., Enzyme kinetics measurement of Gre2p at differe...

Snapshots: No snapshots

Created: 21st Apr 2021 at 08:30, Last updated: 5th May 2021 at 12:02

Selwyn test of Gre2p

Towards Reproducible Enzyme Modeling

Specific activity of Gre2p measured by following the change in absorbance of NADPH at 340 nm for the conversion of nitrononane-2,8-dione (NDK) using different enzyme concentrations.

Submitter: Gudrun Gygli

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Workflow for characterization of enzymes under ...

Study: Spectrophotometric Activity Measurements

Organisms: No organisms

SOPs: Python Workflow for Selwyn test, SOP for specific activity measurements

Snapshots: No snapshots

Created: 21st Apr 2021 at 08:31, Last updated: 5th May 2021 at 12:02

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