Manuscript: structural determinants of substrate binding to NAMPT

This section contains all the raw data and files required for each figures.

DOI: 10.15490/fairdomhub.1.study.666.1

Zenodo URL: None

Created at: 13th Nov 2019 at 17:08

Contents

FIGURE 2: Crystal structures of human NAMPT in complex with NA and PRPP, or NAMN

No description specified

PDB ID 3DHD

Burgos, E. S., Ho, M. C., Almo, S. C., and Schramm, V. L. (2009) A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT. Proceedings of the National Academy of Sciences of the United States of America 106, 13748-13753

  • 3dhd.pdb

PDB ID 6TA0

No description specified

  • 6TA0.pdb

PDB ID 6TA2

No description specified

  • 6TA2.pdb

FIGURE 3: ATP is essential for NAMN formation by NAMPT

No description specified

Fig3: NAMN formation w/ and w/o ATP in WT NAMPT

No description specified

  • Fig3_NAMN formation in WT NAMPT.xlsx

FIGURE 5: The deletion of the β1-β2 loop does not alter the geometry of the active site and yet, abolishes the effect of ATP on NAMPT activity

No description specified

NMN and NAMN formation w/ and w/o ATP in Δ42-51 NAMPT

No description specified

  • Fig4_NAMN-NMN formation in Δ42-51 NAMPT.xlsx

PDB ID 3DHF

Burgos, E. S., Ho, M. C., Almo, S. C., and Schramm, V. L. (2009) A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT. Proceedings of the National Academy of Sciences of the United States of America 106, 13748-13753

  • 3dhf.pdb

PDB ID 6TAC

No description specified

  • 6TAC.pdb

FIGURE 6: SAXS analysis of WT and Δ42-51 NAMPT.

No description specified

SAXS raw data of WT and Δ42-51 NAMPT w/ Nam, NA, PRPP or as apoenzyme

No description specified

  • Fig5_SAXS_rawdata_22102019.xlsx

FIGURE 4: ATP indirectly stabilizes PRPP via pHis247.

No description specified

PDB ID 3DHD

Burgos, E. S., Ho, M. C., Almo, S. C., and Schramm, V. L. (2009) A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT. Proceedings of the National Academy of Sciences of the United States of America 106, 13748-13753

  • 3dkj.pdb

PDB ID 3DKF

Burgos, E. S., Ho, M. C., Almo, S. C., and Schramm, V. L. (2009) A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT. Proceedings of the National Academy of Sciences of the United States of America 106, 13748-13753

  • 3dkf.pdb

PDB ID 3DKL

Burgos, E. S., Ho, M. C., Almo, S. C., and Schramm, V. L. (2009) A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT. Proceedings of the National Academy of Sciences of the United States of America 106, 13748-13753

  • 3dkl.pdb

PDB ID 2E5C

Takahashi, R., Nakamura, S., Nakazawa, T., Minoura, K., Yoshida, T., Nishi, Y., Kobayashi, Y., and Ohkubo, T. (2010) Structure and reaction mechanism of human nicotinamide phosphoribosyltransferase. J Biochem 147, 95-107

  • 2e5c.pdb

PDB ID 6TA0

No description specified

  • 6TA0.pdb

PDB ID 6TAC

No description specified

  • 6TAC.pdb
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Citation
Houry, D. (2019). Manuscript: structural determinants of substrate binding to NAMPT. FAIRDOMHub. https://doi.org/10.15490/FAIRDOMHUB.1.STUDY.666.1
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Created: 13th Nov 2019 at 17:08

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