Investigations
What is an Investigation?
Experimental approaches to evaluate cellular response to Potassium limitation. For this purpose, a series of isogenic strains derived from BY4741 and lacking one (TRK1 or TRK2) or both TRK genes encoding specific potassium uptake systems was constructed using the homologous recombination and Cre-lox system.
Submitter: Simon Borger
Studies: Analysis of proton and potassium fluxes, Experimental approaches to determine the dependence of volume, pH and me..., Ion Flux Changes, Key Cell-Physiological Parameters, Long Term Metabolomic Profile, Proteomic Studies, Pysiological Model, Transcriptional Profile
Assays: 2D-Gel Electrophoresis, Ammonium fluxes, External Potassium Concentration, External pH changes, HPLC-MS, How does internal potassium change (/decrease) during several hours of p..., How starvation affect protein content in yeast cells, LacZ Reporters, Membrane Potential, NMR, Potassium fluxes, Protein Concentration, Protein Identification - MS, Proton fluxes, RT-PCR, Stable membrane potential, Stable pH, Stable potassium concentration, Stable volume, Time Course Micro Array Experiment, Time courses of the internal pH changes, Volume determination during starvation at different times.
