Optical Detection of Interleukin-6 using Liquid Janus Emulsions using Hyperthermophilic Affinity Proteins
DownloadMichelle Chen, Elliot I. Corless, Bevin P. Engelward, Timothy M. Swager
Link to Paper: https://pubs.acs.org/doi/full/10.1021/acsomega.4c03959
Agglutination of liquid Janus emulsions creates optical signals that can be detected and quantified. This study reports the detection and quantitation of Interleukin-6 (IL-6) using emulsions functionalized at the water-organic interface with engineered hyperthermophilic affinity proteins (rcSso7d) derived from thermophilies. Two rcSso7d affinity protein variants were synthesized with a non-canonical azide-functionalized amino acid and detection schemes were compared using one or both binders. The two proteins recognize different epitopes, which can enable simultaneous binding to monomeric IL-6 for agglutination. It is noteworthy that the rsSso7d protein variants, in addition to having superior thermal stability and facile recombinant synthesis in E. coli, these variants show superior performance when compared to commercial antibodies for IL-6.
DOI: None
Zenodo URL: None
Created at: 22nd Aug 2024 at 15:48
Chemical Synthesis - Metadata
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P.SWA-240108-V2_Chemical_Synthesis-Agglutination.docx
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- Chemical_Synthesis-Agglutination.docx
Agglutination Assay - Data Linked
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Nuclear Magnetic Resonance Spectroscopy - Data Linked
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Nuclear Magnetic Resonance Spectroscopy Analysis - Data Linked
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Gel Permeation Chromatography - Data Linked
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Fourier Transform Infrared Spectroscopy - Data Linked
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Pendant Drop Tensiometry - Data Linked
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P.SWA-240105-V1_Pendant-Drop-Tensiometry-Protocol.txt
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- Pendant Drop Tensiometry Protocol.txt
All Metadata
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Created: 22nd Aug 2024 at 15:48
