Optical Detection of Interleukin-6 using Liquid Janus Emulsions using Hyperthermophilic Affinity Proteins
DownloadMichelle Chen, Elliot I. Corless, Bevin P. Engelward, Timothy M. Swager
Link to Paper: https://pubs.acs.org/doi/full/10.1021/acsomega.4c03959
Agglutination of liquid Janus emulsions creates optical signals that can be detected and quantified. This study reports the detection and quantitation of Interleukin-6 (IL-6) using emulsions functionalized at the water-organic interface with engineered hyperthermophilic affinity proteins (rcSso7d) derived from thermophilies. Two rcSso7d affinity protein variants were synthesized with a non-canonical azide-functionalized amino acid and detection schemes were compared using one or both binders. The two proteins recognize different epitopes, which can enable simultaneous binding to monomeric IL-6 for agglutination. It is noteworthy that the rsSso7d protein variants, in addition to having superior thermal stability and facile recombinant synthesis in E. coli, these variants show superior performance when compared to commercial antibodies for IL-6.
DOI: None
Zenodo URL: None
Created at: 22nd Aug 2024 at 15:48
Chemical Synthesis - Metadata
No description specified
P.SWA-240108-V2_Chemical_Synthesis-Agglutination.docx
No description specified
- Chemical_Synthesis-Agglutination.docx
Agglutination Assay - Data Linked
No description specified
Nuclear Magnetic Resonance Spectroscopy - Data Linked
No description specified
Nuclear Magnetic Resonance Spectroscopy Analysis - Data Linked
No description specified
Gel Permeation Chromatography - Data Linked
No description specified
Fourier Transform Infrared Spectroscopy - Data Linked
No description specified
Pendant Drop Tensiometry - Data Linked
No description specified
P.SWA-240105-V1_Pendant-Drop-Tensiometry-Protocol.txt
No description specified
- Pendant Drop Tensiometry Protocol.txt
All Metadata
No description specified
These checksums allow you to check a Snapshot you have downloaded hasn't been modified. For details on how to use these please visit this guide
MD5: ebca9fd523146c94097e5e34f0e6a97f
SHA1: ed62f39bdf6e7e0e070578558fdaf1a9f8d13444
Views: 83 Downloads: 6
Created: 22nd Aug 2024 at 15:48
