Assays

Short read sequencing is a high-throughput next-generation sequencing (NGS) approach used to determine the nucleotide sequence of DNA or RNA fragments, enabling detection of genetic variants, gene expression profiles, or genomic features depending on the library preparation strategy employed. This assay involves fragmentation of input nucleic acids, adapter ligation, and amplification prior to sequencing on short-read platforms (such as Illumina), generating millions of short sequence reads ...

Digitally barcoded Mtb matrix analysis processes barcode sequencing data from Mycobacterium tuberculosis infection experiments. It quantifies relative barcode abundance to track bacterial population dynamics. Input: Barcode sequencing files Output: Processed abundance matrix showing strain or barcode distribution.