Assays

Library preparation converts extracted nucleic acids into sequencing-ready libraries. The process includes fragmentation, adapter ligation, and amplification to generate consistent input for sequencing instruments. Input: TIS, CEL, DNA or RNA sample Output: Indexed sequencing library suitable for sequencing assays.

Short read sequencing is a high-throughput next-generation sequencing (NGS) approach used to determine the nucleotide sequence of DNA or RNA fragments, enabling detection of genetic variants, gene expression profiles, or genomic features depending on the library preparation strategy employed. This assay involves fragmentation of input nucleic acids, adapter ligation, and amplification prior to sequencing on short-read platforms (such as Illumina), generating millions of short sequence reads ...