Assays

This assay quantifies the ability of antibodies to mediate neutrophil-driven phagocytosis of target antigens (such as viral particles, bacteria, or opsonized beads), serving as a functional measure of antibody-mediated innate immune effector activity. Primary human neutrophils or neutrophil-like cell lines are incubated with fluorescently labeled antigen-antibody complexes, and phagocytic uptake is assessed by flow cytometry to determine the proportion of neutrophils that have internalized the ...