Assays

Antibody-dependent neutrophil phagocytosis evaluates antibody-mediated uptake of targets by neutrophils. It measures fluorescence or imaging-based signals to assess innate immune activity. Input: Antibody-labeled target cells and neutrophils Output: Phagocytosis data file indicating neutrophil uptake efficiency.

This assay quantifies the ability of antibodies to mediate neutrophil-driven phagocytosis of target antigens (such as viral particles, bacteria, or opsonized beads), serving as a functional measure of antibody-mediated innate immune effector activity. Primary human neutrophils or neutrophil-like cell lines are incubated with fluorescently labeled antigen-antibody complexes, and phagocytic uptake is assessed by flow cytometry to determine the proportion of neutrophils that have internalized the ...