Assays

DNA extraction is a fundamental laboratory procedure used to isolate and purify genomic DNA from biological samples for downstream molecular analyses. This assay encompasses the collection of relevant metadata associated with the extraction process, including sample identifiers, source material type, extraction method or kit utilized, instrument parameters, operator information, and quality control metrics such as DNA yield and purity (e.g., A260/A280 ratios). Standardized metadata capture ensures ...

Short read sequencing is a high-throughput next-generation sequencing (NGS) approach used to determine the nucleotide sequence of DNA or RNA fragments, enabling detection of genetic variants, gene expression profiles, or genomic features depending on the library preparation strategy employed. This assay involves fragmentation of input nucleic acids, adapter ligation, and amplification prior to sequencing on short-read platforms (such as Illumina), generating millions of short sequence reads ...