Showing for Sample Type: D.BSRA - EI_Fc-Engineered
7 Samples visible to you, out of a total of 7
ID | Title | UID | Name | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Checksum_PrimaryType | Parent | Scientist | SampleCreationDate | Protocol | Type | LightEmission_Plasmid | LightEmission_Reporter | BacterialStrain | MultiplicityOfInfection | InfectedCells | Cells_Vendor | Cells_Catalog# | NumberInfectedCells | ExcitationWavelength | EmissionWavelength | ReadingIntervals | Analyzer | Analyzer_Vendor | Dilution |
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ID | Title | UID | Name | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Checksum_PrimaryType | Parent | Scientist | SampleCreationDate | Protocol | Type | LightEmission_Plasmid | LightEmission_Reporter | BacterialStrain | MultiplicityOfInfection | InfectedCells | Cells_Vendor | Cells_Catalog# | NumberInfectedCells | ExcitationWavelength | EmissionWavelength | ReadingIntervals | Analyzer | Analyzer_Vendor | Dilution |
38978 | D.BSRA-240502ALT-1 | D.BSRA-240502ALT-1 | BSRA-Macrophage | MRA_MBL-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | ea17dee57c0ec75bd0a7890fe1c1d0e9 | MD5 | BAC-240502ALT-1; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2018-04-15T0:0:0+0000 | https://fairdomhub.org/sops/729 | Macrophage Restriction Assay | mCherry for constitutive expression, GFP under anhydrotetracycline inducible promoter | GFP, mCherry | H37Rv Mtb Live/dead Reporter | 1.0x10^6 bacteria per mL of blood | Human monocyte-derived macrophages (MDMs) | N/A | N/A | N/A | N/A | N/A | Fixed and analyzed 3 days post-infection, with additional 16hr post-anhydrotetracycline addition | Columbus Image Data Storage and Analysis System | Revvity | Anhydrotetracycline added at 200 ng/mL to induce GFP expression; monoclonal antibody at 50μg/mL in R10 media without phenol |
38979 | D.BSRA-240502ALT-2 | D.BSRA-240502ALT-2 | BSRA-WBA-1 | WBA_180801-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | 7ec236e5c5759de59b3d158cba4acf1f | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2018-08-01T0:0:0+0000 | https://fairdomhub.org/sops/729 | Whole Blood Restriction Assay | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | Antibody treatments at 25ug/mL in 300µL total volume; Rifampin at 0.25ug/mL |
38980 | D.BSRA-240502ALT-3 | D.BSRA-240502ALT-3 | BSRA-WBA-2 | WBA_180919-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | fc1fb6a448d4081b51af26b2b612b0e4 | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2018-09-19T0:0:0+0000 | https://fairdomhub.org/sops/729 | Whole Blood Restriction Assay | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | Antibody treatments at 25ug/mL in 300µL total volume; Rifampin at 0.25ug/mL |
38981 | D.BSRA-240502ALT-4 | D.BSRA-240502ALT-4 | BSRA-Monocyte-Depletion | Monocyte_Depletion-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | ec3fabbf91fab5fd1f62c4a295bd3aec | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2021-09-23T0:0:0+0000 | https://fairdomhub.org/sops/729 | Blood Immune Cell Depletion (Monocyte) before Whole Blood Restriction | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | 100uL of microbeads per mL of blood, with microbeads buffer-exchanged into 2mM EDTA-PBS |
38982 | D.BSRA-240502ALT-5 | D.BSRA-240502ALT-5 | BSRA-Neutrophil-Depletion | Neutrophil_Depletion-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | 0e3342ec975c1c5f4f1e004f1a7ff553 | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2021-09-23T0:0:0+0000 | https://fairdomhub.org/sops/729 | Blood Immune Cell Depletion (Neutrophil) before Whole Blood Restriction | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | 100uL of microbeads per mL of blood, with microbeads buffer-exchanged into 2mM EDTA-PBS |
38983 | D.BSRA-240502ALT-6 | D.BSRA-240502ALT-6 | BSRA-Complement-Inhibition | 231016_Complement_Inhibition-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2541 | 44e66cde81ec19bfb8b4c805b811c8ed | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2023-10-16T0:0:0+0000 | https://fairdomhub.org/sops/729 | Complement Inhibition before Whole Blood Restriction | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | Cobra venom factor at 50ug/mL |
38984 | D.BSRA-240502ALT-7 | D.BSRA-240502ALT-7 | BSRA-Cytokine-Blockade | 231016_Cytokine_Blockade-BSRA-FcEngineered-EI.xlsx | https://immport.org/shared/study/SDY2542 | 05144ee484c2f320a5082077a54c9b7b | MD5 | BAC-240502ALT-3; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2 | Eddie Irvine | 2023-09-26T0:0:0+0000 | https://fairdomhub.org/sops/729 | Blood Cytokine Blockade before Whole Blood Restriction | https://www.addgene.org/26161/ | Photorhabdus luminescens | H37Rv Mtb-Lux | 1.0x10^6 bacteria per mL of blood | Human whole blood cells | N/A | N/A | N/A | N/A | N/A | Immediately after infection, then every 24 hours up to 120 hours | Tecan Spark 10M | Tecan | Anti-IL-1B and Anti-IFNy at 1ug/mL |