Solvent screening methodology for in situ ABE extractive fermentation


Solvent screening for in situ liquid extraction of products from acetone-butanol-ethanol (ABE) fermentation was carried out, taking into account biological parameters (biocompatibility, bioavailability, and product yield) and ex-traction performance (partition coefficient and selectivity) determined in real fermentation broth. On the basis of different solvent characteristics obtained from literature, 16 compounds from different chemical families were selected and experimentally evaluated for their extraction capabilities in a real ABE fermentation broth system. From these compounds, nine potential solvents were also tested for their biocompatibility towards Clostridium acetobutylicum. Moreover, bioavailability and differences in substrate consumption and total n-butanol production with respect to solvent-free fermentations were quantified for each biocompatible solvent. Product yield was enhanced in the presence of organic solvents having higher affinity for butanol and butyric acid. Applying this methodology, it was found that the Guerbet alcohol 2-butyl-1-octanol presented the best extracting characteristics (the highest partition coefficient (6.76) and the third highest selectivity (644)), the highest butanol yield (27.4 %), and maintained biocompatibility with C. acetobutylicum.


DOI: 10.1007/s00253-014-5634-6

Projects: Ultreia

Publication type: Journal

Journal: Applied Microbiology and Biotechnology

Citation: Appl Microbiol Biotechnol 98(13):5915-5924

Date Published: 1st Jul 2014

Registered Mode: by DOI

Authors: H. González-Peñas, T. A. Lu-Chau, M. T. Moreira, J. M. Lema

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González-Peñas, H., Lu-Chau, T. A., Moreira, M. T., & Lema, J. M. (2014). Solvent screening methodology for in situ ABE extractive fermentation. Applied Microbiology and Biotechnology, 98(13), 5915–5924.

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Created: 8th Sep 2020 at 13:56

Last updated: 8th Sep 2020 at 14:08

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