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Using optical tweezers to position yeast cells in a microfluidic chamber, we were able to observe sustained oscillations in individual isolated cells. Using a detailed kinetic model for the cellular reactions, we simulated the heterogeneity in the response of the individual cells, assuming small differences in a single internal parameter. By operating at two different flow rates per experiment, we observe four of categories of cell behaviour. The present model (gustavsson4) predicts the steady-state ...
Creators: Franco du Preez, Jacky Snoep, Dawie van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online
Using optical tweezers to position yeast cells in a microfluidic chamber, we were able to observe sustained oscillations in individual isolated cells. Using a detailed kinetic model for the cellular reactions, we simulated the heterogeneity in the response of the individual cells, assuming small differences in a single internal parameter. By operating at two different flow rates per experiment, we observe four of categories of cell behaviour. The present model (gustavsson1) predicts the limit ...
Creators: Franco du Preez, Jacky Snoep, David D van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online
Using optical tweezers to position yeast cells in a microfluidic chamber, we were able to observe sustained oscillations in individual isolated cells. Using a detailed kinetic model for the cellular reactions, we simulated the heterogeneity in the response of the individual cells, assuming small differences in a single internal parameter. By operating at two different flow rates per experiment, we observe four of categories of cell behaviour. The present model (gustavsson2) predicts the damped ...
Creators: Franco du Preez, Jacky Snoep, David D van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online
Using optical tweezers to position yeast cells in a microfluidic chamber, we were able to observe sustained oscillations in individual isolated cells. Using a detailed kinetic model for the cellular reactions, we simulated the heterogeneity in the response of the individual cells, assuming small differences in a single internal parameter. By operating at two different flow rates per experiment, we observe four of categories of cell behaviour. The present model (gustavsson3) predicts the steady-state ...
Creators: Franco du Preez, Jacky Snoep, David D van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online
An existing detailed kinetic model for the steady-state behavior of yeast glycolysis was tested for its ability to simulate dynamic behavior. This model (dupreez1) is the basis kinetic model derived from that published by Teusink et al., 2000 (PMID: 10951190).
Creators: Franco du Preez, David D van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online
An existing detailed kinetic model for the steady-state behavior of yeast glycolysis was tested for its ability to simulate dynamic behavior. This model (dupreez2) is an oscillating version of the basis kinetic model (dupreez1) derived from that published by Teusink et al., 2000 (PMID: 10951190).
Creators: Franco du Preez, Jacky Snoep, David D van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online
An existing detailed kinetic model for the steady-state behavior of yeast glycolysis was tested for its ability to simulate dynamic behavior. This model (dupreez3) is an oscillating version of the model published by Teusink et al., 2000 (PMID: 10951190), which describes data for glycolytic intermediates in oscillating yeast cultures reported by Richard et al., 1996 (PMID: 8813760).
Creators: Franco du Preez, Jacky Snoep, David D van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online
An existing detailed kinetic model for the steady-state behavior of yeast glycolysis was tested for its ability to simulate dynamic behavior. This model (dupreez4) is an oscillating version of the model published by Teusink et al., 2000 (PMID: 10951190), which describes data for glycolytic intermediates in oscillating yeast cultures reported by Richard et al., 1996a (PMID: 8813760) as well as the rapid synchronization following the mixing of two yeast cultures that oscillate 180 degrees out of ...
Creators: Franco du Preez, Jacky Snoep, David D van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online
An existing detailed kinetic model for the steady-state behavior of yeast glycolysis was tested for its ability to simulate dynamic behavior. This model (dupreez5) is an oscillating version of the model published by Teusink et al., 2000 (PMID: 10951190), which describes the amplitude bifurcation of oscillating yeast cultures in a CSTR setup reported by Hynne et al., 2001 (PMID: 11744196).
Creators: Franco du Preez, Jacky Snoep, David D van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online
An existing detailed kinetic model for the steady-state behavior of yeast glycolysis was tested for its ability to simulate dynamic behavior. This model (dupreez6) is an oscillating version of the model published by Teusink et al., 2000 (PMID: 10951190), which describes data for glycolytic intermediates in cell free extracts of oscillating yeast cultures reported by Das and Busse, 1991 (PMCID: 1260073).
Creators: Franco du Preez, Jacky Snoep, David D van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online
An existing detailed kinetic model for the steady-state behavior of yeast glycolysis was tested for its ability to simulate dynamic behavior. This model (dupreez7) is an oscillating version of the model published by Teusink et al., 2000 (PMID: 10951190), which describes the fluorescence signal of NADH in oscillating yeast cultures reported by Nielsen et al., 1998 (PMID: 17029704).
Creators: Franco du Preez, Jacky Snoep, David D van Niekerk
Submitter: Franco du Preez
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: JWS Online