Data files

Aiming a greater lactate production and a subsequent steering towards MCC, this operation was conducted. This reactor configuration was similar to the CelluloseXylan_SBR_2ndStage. The first period comprised a feeding phase (6 min; 67% of total feeding) and a reaction phase (4h), which were designed to produce lactate (high load, short time). Then, in the second period a feeding phase (3 min; 33 % of total feeding) and reaction phase (48h) were designed to target the MCC.

In this operation, an SBR fermenting cellulose and xylan was conducted with the aim of enhancing MCC production in two stages: a first stage (CelluloseXylan_SBR_1stStage) targeting lactate and a 2nd stage (current file) targeting MCC. This reactor was inoculated with biomass from CelluloseXylan_SBR_1

In this operation, an SBR fermenting cellulose and xylan was conducted with the aim of enhancing MCC production in two stages: a first stage (current file) targeting lactate and a 2nd stage (CelluloseXylan_SBR_2ndStage) targeting MCC

In this operation, an SBR fermenting cellulose and xylan was conducted with the aim of enhancing MCC production by shortening the HRT

Main parameters of the characterisation of the inoculum from the Lugo WWTP and cow rumen

Concentration of micronutrients and macronutrients added in the reactor feedstock

Libraries & scripts for CRISPR targeting of B. methanolicus

This archive contains the MFA influx software input, output and model files for the paper "Systems-level analysis provides insights on methanol-based production of l-glutamate and its decarboxylation product γ-aminobutyric acid by Bacillus methanolicus".

This archive contains the GSM input, output and model files for the paper "Systems-level analysis provides insights on methanol-based production of l-glutamate and its decarboxylation product γ-aminobutyric acid by Bacillus methanolicus".

The aim of this experiment is to identyfy the bottleneck of xylanfermentation targeting MCC by shortening the HRT from 6 to 1 day

In order to improve the MCC yield obtained from CSTR operation, an SBR operation was conducted varying the HRT aiming a greater caproate and caprilate production. On day 179, a yeast extraction in the feeding was assessed, and finally, on day 252, vitamins were also added in the feeding

When the pseudo-steady state was reached in every SBR operation (SBR HRT 4d (day 34), SBR HRT 2d (day 57), SBR HRT 1d (day 147), SBR HRT 2d+ye (day 253)) the cycle characterisation was performed monitoring the substrate and product evolution during the whole cycle with the aim of assessing the substrate consumption rate and the intermediate products which are produced during the cycle

This archive contains influx software input, output and model files used to estimate in vivo reaction rates of B. methanolicus on methanol, mannitol, and arabitol for the paper "Charting the metabolic landscape of the facultative methylotroph Bacillus methanolicus".