Data files

This file contains the processed LC–MS/MS results for intracellular metabolite quantification using the isotope-ratio approach. It provides the C12/C13 ratios for all metabolites in each sample, calculated relative to the 13C-labelled internal standard. The dataset also includes the retention times and the measurement quality scores for each metabolite, enabling assessment of peak identification and data reliability. The raw LC–MS/MS data used to generate these results are available from the ...

This file contains the data generated for extracellular metabolite quantification using HPLC–MS. It includes the extracted ion chromatograms (EICs) and total ion chromatograms (TICs) for all measured metabolites within the samples and within the calibration standards, as well as the peak heights obtained for both the calibration standards and the extracellular samples. These data support the calculation of metabolite concentrations based on calibration curves.

This file contains metadata associated with the metabolomics sampling procedure. It includes (i) optical density (OD) measurements of each culture at the time of sampling for intracellular and extracellular metabolite quantification, and (ii) the sample label table used for intracellular metabolite analysis. The table provides, for each sample, the sample label/number, collected sample volume, quenching volume, and the mapping to the corresponding biological sample.

This file contains the data generated for glycogen quantification. It includes the raw absorbance measurements at 540 nm for each sample, the corresponding culture optical densities (OD) at the time of sampling, and the calibration curve data (absorbance values and associated glucose standards) used to calculate glucose concentrations from the measured absorbance values.

Biochemical Reaction Networks Biochemical Reaction Networks transform static protein-protein interactions (PPI) network into dynamic, mechanistic models by decomposing each interaction into underlying molecular events like phosphorylation and complex formation. Using SPADAN toolbox (DOI:10.1093/bioinformatics/btad079), we expands PPI network into biochemical reactions network.

Ordinary Differential Equation Model Equations Kinetic descriptions of biochemical reactions related to each renal cell ...

Data submited to Plant Physiology

Immunoblotting gel images, annotations, results

L. infantum promastigotes were administered with EC10 and EC50 concentrations of H80 and Miltefosine. Cells were fractionated into soluble (cyto), insoluble (membranes), and mitochondiral lysates, and analysed through LC-MS/MS Proteomics.

Analysis to determine fold change in mRNA for selected targets (COX-2, IL1B, IL8, 5-LOX, TLR4, iNOS, TNFa)

Illustration of bovine lung explant (BLE) in Tissue Culture Medium (TCM).

Analysis to determine fold change in mRNA for selected targets (COX-2, IL1B, IL8, 5-LOX, TLR4, iNOS, TNFa)

RNA-Seq raw data + sample description

Absorption at 340 nm measured over time in assays performed to determine the IC50 of fruc-1,6-BP for various PGMs as well as calculations perfomed to determine the specific activity [%] normalized to the activity in absence of fruc-1,6-BP

AUC of measured EIC of glc-1,6-BP for each reaction supernatant measured for fig 6b as well as the calculated normalized AUC (normalized to the control).

Data depicted in fig S1

Raw data of absorption at 340 nm during the enzyme assays performed for fig 5 as well as calculations to generate specific activities (inpresence and absence of 100 µM fruc-1,6-BP) from raw data.

Data depicted in fig S2

Raw data of absorption at 340 nm during the enzyme assays performed for fig 2 and 3 as well as calculations to generate specific activities from raw data.

Raw data of absorption at 340 nm during the enzyme assays performed for fig 4 as well as calculations to generate specific activities from raw data.

Raw data of absorption at 340 nm during the enzyme assays performed for fig 5 as well as calculations to generate specific activities and fold changes from raw data.

Raw data of absorption at 340 nm during the enzyme assays performed for fig 6b as well as calculations to generate specific activities and fold changes from raw data.

phylogenetic tree data depicted in fig 1a

phylogenetic tree data depicted in fig 1b

Specific activites of PGM enzymes with and without 40 µM glc-1,6-BP/ 100 µM fruc-1,6-BP.

Specific activites of PGM enzymes with and without 40 µM glc-1,6-BP/ 100 µM fruc-1,6-BP.

Proteinsequences used to generate the phylogenetic trees of fig 1

public file from researchdrive

All genes, DEGs and GO-terms