Investigations
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Objectives: Establishment of the chemoenzymatic process with the best GO-ATA hybrid catalysts. Highlighting of the potential of the process in semi-preparative scale.
Description of Work: The best hybrid catalysts identified in WP3 will be investigated in coupled one-pot reactions selected in WP1, in batch and continuous flow reactors. The productivity of the system will be optimized with response surface methodology (RSM), for parameters such as temperature, duration, substrate concentration ...
Objectives: Identification of amine transaminases (ATAs) able to catalyze efficiently the amination of a desired set of ketones and aldehydes (expected products of GO oxidation). Optimization of the most potent biocatalysts, in terms of catalytic efficiency, stability, availability of functional groups for covalent immobilization.
Description of Work: ATAs from our construct selection (>30 wild-type and variants of both (R)- and (S)-enantioselectivity) with different substrate selectivity will ...
