Studies
What is a Study?
Measure Gre2p activity by following the change in NADPH absorbance at 340 nm for the conversion of different substrates.
Submitter: Gudrun Gygli
Investigation: Workflow for characterization of enzymes under ...
Assays: Kinetic parameters of Gre2p, Selwyn test of Gre2p, Specific activity of Gre2p
Measure binding of reactants to Gre2p by ITC (isothermal titration calorimetry).
Submitter: Gudrun Gygli
Investigation: Workflow for characterization of enzymes under ...
Assays: Analysis of data from ITC experiments (binding), Binding of HK to Gre2p (ITC-BIND), Binding of NADP+ to Gre2p in HEPES Buffer (ITC-BIND), Binding of NADP+ to Gre2p in KPi Buffer (ITC-BIND), Binding of NADP+ to Gre2p in PBS Buffer (ITC-BIND), Binding of NADPH to Gre2p in HEPES Buffer (ITC-BIND), Binding of NADPH to Gre2p in KPi Buffer (ITC-BIND), Binding of NADPH to Gre2p in PBS Buffer (ITC-BIND), Binding of NADPH to Gre2p in Tween-KPi Buffer (ITC-BIND), Binding of NDK to Gre2p (ITC-BIND)
Measure kinetics of reactants by Gre2p with ITC (isothermal titration calorimetry).
Submitter: Gudrun Gygli
Investigation: Workflow for characterization of enzymes under ...
Assays: Analysis of data from ITC experiments (kinetics), Kinetics of the reaction of NDK and NADPH with Gre2p (ITC-MIM) in HEPES ..., Kinetics of the reaction of NDK and NADPH with Gre2p (ITC-MIM) in KPi bu..., Kinetics of the reaction of NDK and NADPH with Gre2p (ITC-MIM) in PBS bu..., Kinetics of the reaction of NDK and NADPH with Gre2p (ITC-MIM) in Tween-..., Kinetics of the reaction of NDK and NADPH with Gre2p (ITC-rSIM) in 3 buf...
Measure homogeneity of an enzyme sample (Gre2p) with DLS (dynamic light scattering).
