Assays

What is an Assay?

Filters

Query

Created At

Updated At

Project

Submitter

Organism

Assay class

Assay type

Technology type

ISA-JSON compliance

1 Assay visible to you, out of a total of 1

Transcriptional profiling of E. coli during anaerobic to aerobic and aerobic to anaerobic transitions

SUMO

This assay involved the determination of transcriptional profiles at 0, 2, 5, 10, 15 and 20 minutes through aerobic to anaerobic gas transitions and anaerobic to aerobic gas transitions. In each case an aerobic or anaerobic steady state was created, RNA sampled (0 min) and then the gas supply changed. RNA samples were then taken from the time at which the gas supply was changed.

For anaerobic conditions 5% CO2, 95% N2 was used.

The full transcriptional dataset is available from ArrayExpress ...

Submitter: Matthew Rolfe

Assay type: Transcriptional Profiling

Technology type: Microarray

Investigation: Dynamical studies for different oxygen availabi...

Study: The Escherichia coli dynamical response to oxyg...

Organisms: Escherichia coli : K-12/MG1655 (wild-type / wild-type)

SOPs: Evans Medium, SUMO RNA isolation from E. coli, SUMO Transcriptional profiling of E. coli, SUMO chemostat conditions

Data files: Dissolved oxygen tension aerobic to anaerobic .pdf, Dissolved oxygen tension anaerobic to aerobic .pdf, Entire transcriptional dataset - aerobic to ana..., Entire transcriptional dataset - anaerobic to a..., Filtered dataset - anaerobic to aerobic .csv, Filtered datset - aerobic to anaerobic transiti...

Snapshots: No snapshots

Created: 30th Apr 2010 at 15:07, Last updated: 8th Nov 2017 at 14:21

Assays

Filters ×

Filters