Protein profiling of SMCs - report

Version 1

The FZB is the lung research center of the Leibniz Association and is therefore committed to the motto “theoria cum praxi”.

The FZB sees its scientific and socio-political task in basic, disease- and patient-oriented lung research and training in this field. The field of research includes chronic inflammatory diseases of the lung of infectious and non-infectious environmental and behavioral origin. Research focuses on tuberculosis, infections with non-tuberculous mycobacteria and other bacterial ...

Human aortic smooth muscel cells (Thermo Fisher Scientific, C-007-5C, LOT: 2164581) were treated with TGB1 or PDGF/L1B to induce the contractile or synthetic SMC phenotype. As part of a multi-omics analysis, we conducted mass spectrometry to quantify protein levels of cell lysates using a HPLC-ESI-MS/MS system (HPLC=high pressure liquid chromatography, Ultimate 3000 UPLC system; ESI=electrospray ionization) and an Orbitrap Exploris™ 480 mass spectrometer. MS data were analyzed and normalized with ...

Cell line: Human aortic smooth muscel cells (Thermo Fisher Scientific, C-007-5C, LOT: 2164581)

Technical replicates: n=4 per condition

Conditions: C3 = TGFB1-treated SMCs C4 = PDGF-BB/IL1B-treated SMCs