_ASSAY_METADATA.TXT Version 1
_p_SUSPHIRE/_I_T11_dCas9SynProm/_S_P1_SynProm_Nbenthamiana/_A_20190208-LUM/
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Assay: _A_20190208-LUM Short Name: 20190208-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay first trial Description: Promoter series containing random sequences and the recognition sequence for guideRNA1 DFR (P1.X series) fused to the developed core promoter minDFR and to luciferase for measuring the expression in the presence or absence of such guideRNA1 DFR. A promoter containing the target sequence in a different position (P1.7.1) and a promoter with the target sequence repeated twice (P2.1) were also assessed. pISA Assay creation date: 2019-02-08 pISA Assay creator: ElenaMG Lab manager: DO Operator: ElenaMG Link to SynBio DM: https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703 Link to protocols: ../protocols/LUCIFERASE Additional Notes: Phenodata: ../../phenodata_Luciferase_20211222.txt Featuredata: Plate ID: 01 DNA introduction system: agrobacteria mediated transient transformation Date of transformation/transfection: 2019-02-04 Treatment: none Date of treatment: 2019-02-04 Date of sampling: 2019-02-08 Date of luminescence assay start: 2019-02-08 Time of luminescence assay start: 12:00 Luminometer: GloMax Multidetection system (Promega) Protocol (luminometer setup): Luminiscence 3x read Time [min] between two measurements (luminometer setup): 2 Integration time [ms] (luminometer setup): 10000 Temperature (luminometer) at start of the experiment [degrees Celsius]: 24 Temperature (luminometer) at the end of the experiment [degrees Celsius]: 24 Time between start of sampling and start of experiment [min]: 60 Silencing supressor added: p19 Notes: Data:
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Created: 10th Jan 2022 at 10:14
Last updated: 7th Nov 2024 at 10:01
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Version 1 (earliest) Created 10th Jan 2022 at 10:15 by Marko Petek
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https://orcid.org/0000-0003-3644-7827PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.
Synthetic biology data management spreadsheet: https://fairdomhub.org/data_files/6036
Programme: NIBSys
Public web page: http://susphire.info/susphireproject/
Start date: 1st Apr 2018
End date: 30th Sep 2021
Short Name: T11_dCas9SynProm Title: Development of synthetic promoters inducible by dCasEV2.1 system Description: The aim of this work is to design a range of synthetic promoters with negligible basal expression that are activated by using the "dead" Cas9 activation system developed in our lab (dCasEV2.1, Selma et al., 2019). Such tool will then be used for creating synthetic regulatory cascades where the expression of the genes for the pheromone biosynthesis can be controlled by a single dCas9 ...
Short Name: P1_SynProm_Nbenthamiana Title: Transient expression in Nicotiana benthamiana of the synthetic promoters developed Description: Different A1 and A2 Different A1 and A2 promoter pieces were designed containing as much random sequence as possible, and the target sequence for the guideRNA 1 of SlDFR promoter. In the same way, some minimal promoters containing only the TATA-Box signal and the 5'UTR region were designed as "core promoter" pieces. Different synthetic promoters were then ...
Submitter: Marko Petek
Investigation: _I_T11_dCas9SynProm
Assays: _A_20190208-LUM, _A_20190717-LUM, _A_20191209-LUM, _A_20200219-LUM, _A_20200302-LUM, _A_20200818-LUM, _A_20201112-LUM, _A_20211221-LUM, _S_P1_SynProm_Nbenthamiana-files
Short Name: 20190208-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay first trial Description: Promoter series containing random sequences and the recognition sequence for guideRNA1 DFR (P1.X series) fused to the developed core promoter minDFR and to luciferase for measuring the expression in the presence or absence of such guideRNA1 DFR. A promoter containing the target sequence in a different position (P1.7.1) and a promoter with the target sequence repeated twice (P2.1) were also ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T11_dCas9SynProm
Study: _S_P1_SynProm_Nbenthamiana
