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2 Assays visible to you, out of a total of 2

Kinetics of the reaction of NDK and NADPH with Gre2p (ITC-MIM) in HEPES buffer

Towards Reproducible Enzyme Modeling

ITC multiple injection (MIM) experiments to determine the kinetic parameters of NDK (nitrononane-2,8-dione) and NADPH with Gre2p in 100 mM HEPES buffer pH 7.5 Data from ITC recurrent single injection (rSIM) experiments were used to achieve proper fitting of kcat values.

Submitter: Gudrun Gygli

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Workflow for characterization of enzymes under ...

Study: ITC kinetic experiments (enzyme activity)

Organisms: No organisms

SOPs: SOP for ITC-MIM experiments, SOP for usage of the ITC

Data files: ITC-MIM experiment for the reaction of NADPH an..., ITC-MIM experiment for the reaction of NADPH an...

Snapshots: No snapshots

Created: 21st Apr 2021 at 12:26, Last updated: 5th May 2021 at 12:02

Analysis of data from ITC experiments (kinetics)

Towards Reproducible Enzyme Modeling

Python workflow for the analysis of ITC-BIND, ITC-MIM and ITC-(r)SIM experiments. Organized in a *.zip folder. Requires the following directory structure:

./ITC_analysis.py ./input/BINDING/.apj ./input/BINDING/.csv ./input/KINETICS/.apj ./input/KINETICS/.csv ./scripts/binding_neu.py ./scripts/kinetics_neu.py

And can be executed by running python ITC_analysis.py in the directory. Filenames for the input *.apj and *.csv files are defined in ITC_analysis.py. The output directory is written by ...