Cell free extract, with Mn, no NAD recycling

Conversion of XYL to KG in a cell free extract of Caulobacter crescentus, with 0.15 mM Mn2+ added, but no NAD recycling, metabolites measured enzymatically.
https://jjj.bio.vu.nl/models/experiments/shen2020_fig4c/simulate

DOI: 10.15490/fairdomhub.1.assay.994.2

Created at: 8th Jan 2020 at 16:23

Contents

CFE Mn no-NADrec data

Data files for the conversion of XYL to KG, in Caulobacter crescentus cell free extract, without NAD recycling, but with additional Mn2+ (0.15 mM) added.

  • cfe-Mn-noRec.xlsx

CFE Mn noNADrec

Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG in cell free extract. If the Mathematica notebook is downloaded and the data file is downloaded in the same directory, then the notebook can be evaluated, and the figure in the manuscript for the cell free extract with added Mn, but no NAD rec, will be reproduced.

  • cfe-Mn-noRec-SEEK.nb

CFE analysis (shen2)

Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG. Protein levels need to be adapted to CFE levels, see SED-ML scripts

  • shen2.xml
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Citation
Snoep, J., & Shen, L. (2020). Cell free extract, with Mn, no NAD recycling. FAIRDOMHub. http://doi.org/10.15490/FAIRDOMHUB.1.ASSAY.994.2
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Created: 8th Jan 2020 at 16:23

Last updated: 8th Jan 2020 at 16:23

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