Cell free extract, with Mn, no NAD recycling

Conversion of XYL to KG in a cell free extract of Caulobacter crescentus, with 0.15 mM Mn2+ added, but no NAD recycling, metabolites measured enzymatically. https://jjj.bio.vu.nl/models/experiments/shen2019_fig4c/simulate

DOI: 10.15490/fairdomhub.1.assay.994.1

Zenodo URL: None

Created at: 8th Jan 2020 at 13:19

Contents

CFE Mn no-NADrec data

Data files for the conversion of XYL to KG, in Caulobacter crescentus cell free extract, without NAD recycling, but with additional Mn2+ (0.15 mM) added.

cfe-Mn-noRec.xlsx

CFE Mn noNADrec

Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG in cell free extract. If the Mathematica notebook is downloaded and the data file is downloaded in the same directory, then the notebook can be evaluated, and the figure in the manuscript for the cell free extract with added Mn, but no NAD rec, will be reproduced.

cfe-Mn-noRec-SEEK.nb

CFE analysis (shen2)

Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG. Protein levels need to be adapted to CFE levels, see SED-ML scripts

shen2.xml

Fingerprints

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MD5: c8d14ada8177e33c45826a09f3350fce

SHA1: e04d5dc272d69ed2363a78673ae1cb49b5ba7c59

Citation

Snoep, J., & Shen, L. (2020). Cell free extract, with Mn, no NAD recycling. FAIRDOMHub. https://doi.org/10.15490/FAIRDOMHUB.1.ASSAY.994.1

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Created: 8th Jan 2020 at 13:19

Last updated: 8th Jan 2020 at 13:19

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