SOPs

4 SOPs visible to you, out of a total of 4

SOP_Preparation of cell lysates

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment, Target verification/ Specificity of 3BrP in gly...

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Created: 13th Jul 2023 at 12:52

SOP_Inhibitor titration intact cells

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment, Target verification/ Specificity of 3BrP in gly...

Download

Created: 13th Jul 2023 at 12:49

SOP_Quantification of glycolytic flux (glucose, lactate)

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment

Download

Created: 12th Jul 2023 at 13:05

SOP_Cancer cell lines_standard cell culture

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment, Target verification/ Specificity of 3BrP in gly...

Download

Created: 12th Jul 2023 at 13:03

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