SOPs

2 SOPs visible to you, out of a total of 2

SOP describing how to perform an ITC binding experiment with a MicroCal PEAQ ITC by Malvern. Note: the DOIs from the Chemotion database for the synthesis of HK and NDK are missing in this version beacuse the DOIs do not exist yet (as of 27.04.21)

Creators: Gudrun Gygli, Felix Ott

Submitter: Gudrun Gygli

Investigations: Workflow for characterization of enzymes under ...

Studies: ITC binding experiments

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Created: 27th Apr 2021 at 09:07, Last updated: 5th May 2021 at 12:03

SOP for usage of the ITC

Towards Reproducible Enzyme Modeling

SOP describing the steps for use of the ITC device (MicroCal PEAQ ITC by Malvern).

Creator: Gudrun Gygli

Submitter: Gudrun Gygli

Investigations: Workflow for characterization of enzymes under ...

Studies: ITC binding experiments, ITC kinetic experiments (enzyme activity)

Assays: Binding of HK to Gre2p (ITC-BIND), Binding of NADP+ to Gre2p in HEPES Buffer (ITC-..., Binding of NADP+ to Gre2p in KPi Buffer (ITC-BIND), Binding of NADP+ to Gre2p in PBS Buffer (ITC-BIND), Binding of NADPH to Gre2p in HEPES Buffer (ITC-..., Binding of NADPH to Gre2p in KPi Buffer (ITC-BIND), Binding of NADPH to Gre2p in PBS Buffer (ITC-BIND), Binding of NADPH to Gre2p in Tween-KPi Buffer (..., Binding of NDK to Gre2p (ITC-BIND), Kinetics of the reaction of NDK and NADPH with ..., Kinetics of the reaction of NDK and NADPH with ..., Kinetics of the reaction of NDK and NADPH with ..., Kinetics of the reaction of NDK and NADPH with ..., Kinetics of the reaction of NDK and NADPH with ...

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Created: 27th Apr 2021 at 08:11, Last updated: 5th May 2021 at 12:03

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