Assays

Files connected to study: _S_P1_TransientExp_Nb

Short Name: AgroinfFinalConstructsdCas9EV-GCMS Assay Class: WET Assay Type: GCMS Title: Agroinfiltration of final constructs for copper-inducible pathway in Nicotiana benthamiana leaves Description: Two constructs were made containing the three genes of the moth pheromone pathway under the regulation of synthetic promoters made in T11, nptII selection marker and with/without the gRNA1 for activation. In parallel, another construct containing the copper-inducible dCas9EV system with/without the ...

Short Name: AgroinfGuidedPathwaywithConstitutivedCas9EV-GCMS Assay Class: WET Assay Type: GCMS Title: Agroinfiltration of guided pathway for moth pheromones production with constitutive dCas9EV system in Nicotiana benthamiana leaves Description: Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. In the constructs it was also included the guideRNA1 and nptII ...

Short Name: AgroinfNonGuidedPathwaywithCopperInducibledCas9EV-GCMS Assay Class: WET Assay Type: GCMS Title: Agroinfiltration of non-guided pathway for moth pheromones production with copper-inducible dCas9EV system in Nicotiana benthamiana leaves Description: Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. The constructs were first tested transiently in ...

Short Name: AgroinfNonGuidedPathwayywithConstitutivedCas9EV-GCMS Assay Class: WET Assay Type: GCMS Title: Agroinfiltration of nonguided pathway for moth pheromones production with constitutive dCAs9EV system in Nicotiana benthamiana leaves Description: Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. The constructs were first tested transiently in Nicotiana ...