![](/assets/avatars/avatar-study-cf2295264451598f1ffcf0379368bdb8dd86a5f58879d5cc1649ce92767121fb.png)
Deciphering the tight Metabolite-Level Regulation of Glucose-1-Phosphate Adenylyltransferase (GlgC) for Glycogen Synthesis in Cyanobacteria
![](/assets/crystal_project/16x16/actions/gear-f11bc5ea57c7be49247f17f04b2aafc6e609a120837c7c2fd4cfc8e580698136.png)
Abstract The enzyme Glucose-1-phosphate adenylyltransferase (GlgC, EC:2.7.7.27) catalyses the first step in glycogen synthesis by converting glucose-1-phosphate into ADP-Glucose, which is added in turn to a growing glycogen chain by glycogen synthases. Thus far, the in vitro study of GlgC were mainly performed using colorimetric or radiolabel-based phosphate release assays, limiting the option for analysing this reaction. With this work, we present a novel in vitro continuous assay coupling the subsequent glycogen synthase reaction to the GlgC reaction, thus simulating the process of glycogen synthesis in vivo. Using this assay, we revisited GlgC catalytic parameters and screened for metabolites that affect GlgC activity in Synechocystis sp. PCC 6803. We also describe in further detail the antagonistic interplay between the GlgC activator, 3-PGA and the inhibitor, inorganic phosphate, revealing the intricate mechanism by which glycogen formation responds to fluctuations in carbon and energy supply in cyanobacteria.
Here you will find data files for the results presented in this work, in Excel (.xlsx) and Graphpad (.pzf) format.
SEEK ID: https://fairdomhub.org/studies/1296
hidden item
Projects: SCyCode The Autotrophy-Heterotrophy Switch in Cyanobacteria: Coherent De...
Study position:
![help](/assets/famfamfam_silk/information-8cfe563be76fb11b27c8ba778f5d40ce6800b244d6ca58ef6e9973308efbe534.png)
Creator
Submitter
Views: 100
Created: 5th Jul 2024 at 19:22
Last updated: 23rd Jul 2024 at 22:15
![help](/assets/famfamfam_silk/information-8cfe563be76fb11b27c8ba778f5d40ce6800b244d6ca58ef6e9973308efbe534.png)
This item has not yet been tagged.