Assays

Short Name: 08_mapReadsToAs1-STAR Assay Class: DRY Assay Type: STAR Title: Map RNAseq reads to de-novo assembly1 Description: STAR mapper was used to map back P citri RNA-Seq reads used for generation of the de-novo assembly1. Phenodata: ../../phenodata_20181003.txt Featuredata: Data:

Short Name: 09_As1_tr_limmaDE-R Assay Class: DRY Assay Type: R Title: Assembly1 transcripts differential expression analysis using limma voom package Description: Using output files of mapReadsToAs1 generated by STAR, the counts were analyzed for differential expression in R using limma voom approach. Analyst: MPE Phenodata: ../../phenodata_20190523.txt Featuredata: Data: ../_A_08_mapReadsToAs1-STAR/output

Files connected to study: _S_P4_Pcitri_tr1

Short Name: 05_assembly1-rnaSPAdes Assay Class: DRY Assay Type: rnaSPAdes Title: P citri de-novo transcriptome assembly Description: P citri VF RNA-seq samples were assembled using rnaSPAdes assembler Phenodata: ../../phenodata_20181003.txt Featuredata: Data:

Short Name: 06_assembly1-IPS Assay Class: DRY Assay Type: IPS Title: Assembly1 InterProScan Description: InterProScan run for assembly1 transcripts. AA sequences were obtained by runing transeq and filtering to 3 longest ORFs per transcript. Phenodata: ../../phenodata_20181003.txt Featuredata: Data: ../_A_assembly1-rnaSPAdes/output/soft_filtered_transcripts.fasta

Short Name: 07_mapAs1ToGenome-STAR Assay Class: DRY Assay Type: STAR Title: Map Assembly1 transcripts to P citri reference genome Description: STARlong mapper was used to map de-novo assembled transcripts to P. citri reference genome. Phenodata: ../../phenodata_20181003.txt Featuredata: Data: