Assays

3 Assays visible to you, out of a total of 3

Aerobic and anaerobic characterization of MG1655 and mutant strains with regard to growth, by-product formation and cAMP concentrations.

SUMO

Glucose transporter mutants were analyzed under aerobic and aerobic conditions in batch cultures with glucose as substrate. Acetate formation rates and glucose consumption rates were measured, as well as extracellular cAMP concentrations.

Submitter: Sonja Steinsiek

Assay type: Extracellular Metabolite Concentration

Technology type: Technology Type

Investigation: Analysis of the glucose transport in Escherichi...

Study: Characterization of mutant strains with defects...

Organisms: Escherichia coli : MG1655 (del LAM;del rph-1 / pyrimidine starvation )

SOPs: Measurement of extracellular metabolites and 1 hidden item

Data files: Growth, by-product formation and glucose consum..., Growth, yield and cAMP excretion under anaerobi...

Snapshots: No snapshots

Created: 17th Jan 2013 at 12:18, Last updated: 8th Nov 2017 at 14:21

ArcA phosphorylation at different aerobiosis levels (steady states)

SUMO

ArcA phosphorylation in chemostat cultures grown at different aerobiosis levels was quantitated by Phos-tag SDS-PAGE gel analysis and subsequent immunodetection of ArcA.

Submitter: Matthew Rolfe

Assay type: Experimental Assay Type

Technology type: SDS-PAGE

Investigation: Steady state studies for different oxygen avail...

Study: The Escherichia coli steady state response to o...

Organisms: Escherichia coli : MG1655 (del LAM;del rph-1 / pyrimidine starvation )

SOPs: Determination of protein phosphorylation levels..., Evans Medium, SUMO chemostat conditions

Data files: No Data files

Snapshots: No snapshots

Created: 30th Jan 2013 at 15:22, Last updated: 8th Nov 2017 at 14:21