Assays
What is an Assay?Filters
Query
Created At
Updated At
Project
BaCell-SysMO1
Submitter
Ulf Liebal1
Organism
Bacillus subtilis1
Assay class
Experimental assay1
Assay type
Proteomics1
Technology type
Technology Type1
1
Assay visible to you, out of a total of 1
We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity. SigmaB itself is placed downstream of Pspac, inducible by IPTG. The lacZ reporter gene is downstream of Pctc promoter. IPTG concentrations of 0.1, 0.2 and 1 mM are added in mid-exponential phase at an OD of appr. 0.3. The whole experiment runs for about eight hours.
Submitter: Ulf Liebal
Assay type: Proteomics
Technology type: Technology Type
Investigation: The transition from growing to non-growing Baci...
