Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T

Abstract:
        Clostridium beijerinckii is a relatively widely studied, yet non-model, bacterium. While 246 genome assemblies of its various strains are available currently, the diversity of the whole species has not been studied, and it has only been analyzed in part for a missing genome of the type strain. Here, we sequenced and assembled the complete genome of the type strain Clostridium beijerinckii DSM 791T, composed of a circular chromosome and a circular megaplasmid, and used it for a comparison with other genomes to evaluate diversity and capture the evolution of the whole species. We found that strains WB53 and HUN142 were misidentified and did not belong to the Clostridium beijerinckii species. Additionally, we filtered possibly misassembled genomes, and we used the remaining 237 high-quality genomes to define the pangenome of the whole species. By its functional annotation, we showed that the core genome contains genes responsible for basic metabolism, while the accessory genome has genes affecting final phenotype that may vary among different strains. We used the core genome to reconstruct the phylogeny of the species and showed its great diversity, which complicates the identification of particular strains, yet hides possibilities to reveal hitherto unreported phenotypic features and processes utilizable in biotechnology.

SEEK ID: https://fairdomhub.org/publications/649

DOI: 10.3390/pr9071196

Projects: Clostridium beijerinckii pan-genome

Publication type: Journal

Journal: Processes

Citation: Processes 9(7):1196

Date Published: 1st Jul 2021

Registered Mode: by DOI

Authors: Karel Sedlar, Marketa Nykrynova, Matej Bezdicek, Barbora Branska, Martina Lengerova, Petra Patakova, Helena Skutkova

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Citation
Sedlar, K., Nykrynova, M., Bezdicek, M., Branska, B., Lengerova, M., Patakova, P., & Skutkova, H. (2021). Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T. In Processes (Vol. 9, Issue 7, p. 1196). MDPI AG. https://doi.org/10.3390/pr9071196
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Created: 11th Aug 2022 at 14:38

Last updated: 11th Aug 2022 at 14:40

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