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SysMO1
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KOSMOBAC1
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CSIC Vigo1
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Spain1
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including:
- Dynamic modelling
- Parameter estimation
- Optimal experimental design
- Dynamic optimization1
ODE8
Computational and theoretical biology6
Biochemistry and protein analysis5
Matlab5
Molecular Biology4
linux4
differential algebraic equations4
fluorescence spectroscopy4
Stochastic models4
Computational Systems Biology4
Microbiology3
Partial differential equations3
data modeling3
molecular biological techniques (RNA/DNA techniques3
C programming3
Membrane protein biochemistry3
Genetics2
Bioinformatics2
Transcriptomics2
Single Cell analysis2
SBML2
Mathematica2
Fermentation2
rtPCR2
DIVA2
Fluorescence and confocal microscopy2
enzymatic analyses2
qPCR2
quantitative western blot analysis2
Deterministic models2
Biochemistry of substrate transport. molecular biological techniques (RNA/DNA techniques analyses of transport across cell membranes heterologous expression of transporters2
Data Management1
Genomics1
Isolation purification and separation1
Model organisms1
Spectroscopy and structural analysis1
Algebraic equations1
Linear equations1
Perl1
Python1
Microarray analysis1
Chip-chip1
Chromatography1
Material balance based modeling1
stimulus response experiments1
ProMOT1
evaluation of process dynamics1
continuous cultivation1
Enzyme assay1
pulsed - FRAP1
Protein chemical methods (protein overproduction1
purification1
quantitative Western analyses)1
Northern analyses)1
Proteomics (2D-PAGE)1
mutant strain generation1
Fluorecence based reporter gene analyses/single cell analyses1
FRAP1
XPPAUT1
including:- Dynamic modelling- Parameter estimation- Optimal experimental design- Dynamic optimization1
Molecular biology techniques (RNA/DNA)1
Fluorescence and confocal microscopy
FRAP/FLIP1
The Membrane Enzymology group focuses on the molecular mechanisms of transport and signalling across biological membranes. In other words: How do molecules permeate biological membranes and how at the level of protein molecules are environmental signals s1
engineering principles and approaches from molecular biology and synthetic chemistry are applied. Confocal microscopy and various spectroscopic methods are used to analyze processes at the single cell or even single molecule level.1
Chemical cross-linking1
Site-directed mutagenesis1
PDE1
C programing1
transcriptional analysis (Northern blots1
quantitative RT-PCR); quantitative Western blot analysis; protein purification; reconstitution of membrane-integrated proteins; Biochemistry of substrate transport; Fluorecence based reporter gene analyses/single cell analyses1
surface plasmon resonance spectroscopy; site-directed spin labeling and EPR analysis;1
Dynamic modelling1
methods of nonlinear time series analysis1
programming in C1
site-directed spin labeling and EPR spectroscopy1
isothermal titration calorimetry1
molecular biological techniques (RNA/DNA techniques)1
spectroscopy1
site-directed and random mutagenesis1
plasmon resonance spectroscopy1
reporter gene analyses1
Parameter estimation1
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We are very interested in applying a systems approach (i.e. model-based concepts and related computational tools) to problems from the biological domain. In particular, we are doing research in computational systems biology, targetting the following topics:
- Parameter estimation (inverse problems, model calibration) in biochemical pathways
- Optimal experimental design (optimal dynamic experiments) for Systems Biology
- Dynamic optimization (optimal control) of biosystems and bioprocesses
...