Studies
What is a Study?
PPK was isolated and it was kinetically characterized in initial rate kinetic experiments. A rate equation was parameterized and used to simulate enzyme incubations
Submitter: Jacky Snoep
Investigation: Polyphosphate kinases in Sulfolobus acidocaldarius
One pot cascade - pathway analysis for the purified Caulinobacter crescentus Weimberg pathway enzymes. Effect of co-factor recycling, removal of XLA, and optimisation on Xylose to aKG is studied.
https://jjj.bio.vu.nl/models/experiments/shen2020_fig3a/simulate https://jjj.bio.vu.nl/models/experiments/shen2020_fig3b/simulate https://jjj.bio.vu.nl/models/experiments/shen2020_fig3c/simulate https://jjj.bio.vu.nl/models/experiments/shen2020_fig3d/simulate
Submitter: Jacky Snoep
Investigation: Caulobacter crescentus Weimberg pathway
Assays: One pot cascade 10, One pot cascade 12, One pot cascade 13, One pot cascade 16
Initial rate kinetics for the purified Caulinobacter crescentus Weimberg pathway enzymes, including substrate dependence, and product inhibition.
Submitter: Jacky Snoep
Investigation: Caulobacter crescentus Weimberg pathway
Progress curves for the purified Caulinobacter crescentus Weimberg pathway enzymes. Each reaction is followed up to completion and then the next enzyme in the pathway is added, i.e. XDH-XLA-XAD-KDXD and finally KGSADH
Submitter: Jacky Snoep
Investigation: Caulobacter crescentus Weimberg pathway
Assays: Progress curve KDXD, Progress curve KGSADH, Progress curve XAD, Progress curve XDH, Progress curve XLA, Progress curves combined
Cell free extract - pathway analysis for Caulinobacter crescentus Weimberg pathway enzymes. Effect of co-factor recycling, and Mn2+ on Xylose to aKG conversion is studied.
