Publications

96 Publications visible to you, out of a total of 96

Abstract

Not specified

Authors: Y.-J. Tan, P.-Y. Tham, D. Z. L. Chan, C.-F. Chou, S. Shen, B. C. Fielding, T. H. P. Tan, S. G. Lim, W. Hong

Date Published: 13th Jul 2005

Publication Type: Journal

Abstract

Not specified

Authors: Yumiko Imai, Keiji Kuba, Shuan Rao, Yi Huan, Feng Guo, Bin Guan, Peng Yang, Renu Sarao, Teiji Wada, Howard Leong-Poi, Michael A. Crackower, Akiyoshi Fukamizu, Chi-Chung Hui, Lutz Hein, Stefan Uhlig, Arthur S. Slutsky, Chengyu Jiang, Josef M. Penninger

Date Published: 1st Jul 2005

Publication Type: Journal

Abstract (Expand)

A novel coronavirus, the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), was identified as the causative agent of SARS. The profile of specific antibodies to individual proteins of the virus is critical to the development of vaccine and diagnostic tools. In this study, 13 recombinant proteins associated with four structural proteins (S, E, M and N) and five putative uncharacterized proteins (3a, 3b, 6, 7a and 9b) of the SARS-CoV were prepared and used for screening and monitoring their specific IgG antibodies in SARS patient sera by protein microarray. Antibodies to proteins S, 3a, N and 9b were detected in the sera from convalescent-phase SARS patients, whereas those to proteins E, M, 3b, 6 and 7a were undetected. In the detectable specific antibodies, anti-S and anti-N were dominant and could persist in the sera of SARS patients until week 30. Among the rabbit antisera to recombinant proteins S3, N, 3a and 9b, only anti-S3 serum showed significant neutralizing activity to the SARS-CoV infection in Vero E6 cells. The results suggest (1) that anti-S and anti-N antibodies are diagnostic markers and in particular that S3 is immunogenic and therefore is a good candidate as a subunit vaccine antigen; and (2) that, from a virus structure viewpoint, the presence in some human sera of antibodies reacting with two recombinant polypeptides, 3a and 9b, supports the hypothesis that they are synthesized during the virus cycle.

Authors: Maofeng Qiu, Yuling Shi, Zhaobiao Guo, Zeliang Chen, Rongqiao He, Runsheng Chen, Dongsheng Zhou, Erhei Dai, Xiaoyi Wang, Bingyin Si, Yajun Song, Jingxiang Li, Ling Yang, Jin Wang, Hongxia Wang, Xin Pang, Junhui Zhai, Zongmin Du, Ying Liu, Yong Zhang, Linhai Li, Jian Wang, Bing Sun, Ruifu Yang

Date Published: 1st May 2005

Publication Type: Journal

Abstract

Not specified

Authors: Y.-J. Tan, E. Teng, S. Shen, T. H. P. Tan, P.-Y. Goh, B. C. Fielding, E.-E. Ooi, H.-C. Tan, S. G. Lim, W. Hong

Date Published: 11th Jun 2004

Publication Type: Journal

Abstract

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Authors: Volker Thiel, Konstantin A. Ivanov, Ákos Putics, Tobias Hertzig, Barbara Schelle, Sonja Bayer, Benedikt Weißbrich, Eric J. Snijder, Holger Rabenau, Hans Wilhelm Doerr, Alexander E. Gorbalenya, John Ziebuhr

Date Published: 1st Sep 2003

Publication Type: Journal

Abstract

Not specified

Author: Stuart G. Siddell

Date Published: 1995

Publication Type: InCollection

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