SOPs

What is a SOP?

7 SOPs visible to you, out of a total of 7

Anaerobic media composition

MOSES

SOP for growing yeast in anaerobic conditions

Creator: Maksim Zakhartsev

Submitter: Maksim Zakhartsev

Investigations: Kinetic analysis of metabolic system using tran..., Steady state metabolic fluxes and metabolite co...

Studies: Metabolic perturbation of the steady state cult..., Steady state concentrations of metabolites in y..., Steady state fluxes in yeast Saccharomyces cere...

Assays: Biomass weight during glucose pulse, Cellular size and granularity during glucose pulse, Dynamics of extracellular metabolites during gl..., Dynamics of intracellular metabolites during gl..., Dynamics of macromolecules during glucose pulse, Steady state concentrations of extracellular me..., Steady state concentrations of intracellular me..., Steady state extracellular fluxes in anaerobic ...

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Created: 2nd Mar 2010 at 22:12, Last updated: 1st Mar 2012 at 10:59

Sampling of biomass

MOSES

Biomass sampling - SOP for sampling of biomass

Creator: Maksim Zakhartsev

Submitter: Maksim Zakhartsev

Investigations: Kinetic analysis of metabolic system using tran..., Steady state metabolic fluxes and metabolite co...

Studies: Metabolic perturbation of the steady state cult..., Steady state concentrations of metabolites in y..., Steady state fluxes in yeast Saccharomyces cere...

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Created: 2nd Mar 2010 at 22:14, Last updated: 1st Mar 2012 at 10:57

Perturbation conditions

MOSES

Metabolic perturbations - SOP for metabolic perturbations (i.e. glucose pulse)

Creator: Maksim Zakhartsev

Submitter: Maksim Zakhartsev

Investigations: Kinetic analysis of metabolic system using tran...

Studies: Metabolic perturbation of the steady state cult...

Assays: Cellular size and granularity during glucose pulse, Dynamics of extracellular metabolites during gl..., Dynamics of intracellular metabolites during gl..., Dynamics of macromolecules during glucose pulse

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Created: 2nd Mar 2010 at 22:15, Last updated: 1st Mar 2012 at 10:55

Yeast strains

MOSES

Yeast strains used in the project

Creator: Maksim Zakhartsev

Submitter: Maksim Zakhartsev

Investigations: Kinetic analysis of metabolic system using tran..., Steady state metabolic fluxes and metabolite co...

Studies: Metabolic perturbation of the steady state cult..., Steady state concentrations of metabolites in y..., Steady state fluxes in yeast Saccharomyces cere...

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Created: 2nd Mar 2010 at 22:28, Last updated: 1st Mar 2012 at 10:47

Determination of the kinetic parameters of the purified glycolytic isoenzymes of Saccharomyces cerevisiae

MOSES

General protocol for measuring the kinetic parameters of the purified glycolytic enzymes from Saccharomyces cerevisiae - SOP for measuring the kinetic parameters of the purified glycolytic isoenzymes

Creator: Hanan Messiha

Submitter: Maksim Zakhartsev

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 2nd Mar 2010 at 22:29, Last updated: 1st Mar 2012 at 10:44

Determination of (cytosolic) enzyme activities

MOSES

Sample preparation - SOP for sampling, preparation of cell-free extracts, and determination of total extracted protein

Creator: Femke Mensonides

Submitter: Maksim Zakhartsev

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 2nd Mar 2010 at 22:22, Last updated: 1st Mar 2012 at 10:40

Determination of intracellular metabolites

MOSES

Sample preparation - SOP for sampling, preparation of cell extracts, and general assay set-up

Creator: Femke Mensonides

Submitter: Maksim Zakhartsev

Investigations: Kinetic analysis of metabolic system using tran..., Steady state metabolic fluxes and metabolite co...

Studies: Metabolic perturbation of the steady state cult..., Steady state concentrations of metabolites in y...

Assays: Cellular size and granularity during glucose pulse, Dynamics of intracellular metabolites during gl..., Dynamics of macromolecules during glucose pulse, Steady state concentrations of intracellular me...

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Created: 2nd Mar 2010 at 22:18, Last updated: 1st Mar 2012 at 10:35

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