Roland Wohlgemuth

Overview
Related items

About Roland Wohlgemuth:

No description specified

SEEK ID: https://fairdomhub.org/people/446

Location: Switzerland

ORCID: Not specified

Joined: 28th Aug 2014

Expertise: Not specified

Tools: Not specified

Their tags

This person has not yet tagged anything.

Related items

Advanced Programmes list for this Person with search and filtering

e:Bio

e:Bio - Innovations Competition Systems Biology

Projects: SulfoSys - Biotec, SBEpo - Systems Biology of Erythropoietin

Web page: http://www.fona.de/en/14276

Advanced Projects list for this Person with search and filtering

SulfoSys - Biotec

Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.

Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012) ...

Programme: e:Bio

Public web page: http://www.sulfosys.com/

Organisms: Sulfolobus solfataricus

Advanced Institutions list for this Person with search and filtering

Sigma Aldrich ( Swiss)

ROR ID: Not specified

Department: Not specified

Country: Switzerland

City: St. Gallen

Web page: https://www.sigmaaldrich.com/switzerland-schweiz.html

Advanced Publications list for this Person with search and filtering

Metabolic thermodynamics: pertinent reference state and energy potentials

Thermodynamics, Book on Thermodynamics, and kinetics, Thermotables for biochemistry

(Show All)

Abstract (Expand)

Chemical potentials (molar Gibbs energies) are usually extrapolated to the remote physical–chemical reference state and then stored. Subsequent use under in vivo conditions requires a similarly … conditions requires a similarly substantial, reverse extrapolation, again with significant potential errors. In order to shrink both extrapolations drastically and thereby enhance both biological meaning and accuracy, we propose a transformation to a more biological reference state: pH = 7, pMg = 3, 99.5% water, with 1 m m each of the additional ‘precursors’ inorganic phosphate, sulfate, ammonium, and bicarbonate, and with twin temperatures 37 and 25 °C, ionic strength 0.15 m and m m as concentration unit. These precursors substitute for reference compounds alien to biology such as H 2 at 1 bar, and solid graphite, sulfur, and phosphorus. The standard chemical potentials are herewith increased by the magnitudes of the chemical potentials of protons, Mg 2+ , water, and the four precursors, each multiplied by the number of corresponding atoms in the molecule. This defines standard ‘metabolic potentials’. We make these potentials findable and accessible as 1360 collated standard chemical potentials for 320 compounds of biochemical interest at the twin metabolic reference states. We do this for 3 reference pH's: We present the metabolic reference state as a convenient anchor , not a universal intracellular milieu. All datasets must continue to report the actual experimental state ( T , pH, pMg, I , osmolarity, concentrations), yet aim at (also) reporting parameter values for this anchor state; we supply algorithms to transform between states. This preserves interoperability across diverse organelles, media and between enzymology and chemical engineering, while facilitating reuse.

Authors: Hans V. Westerhoff, Barbara M. Bakker, Andreas S. Bommarius, Maria Luz Cárdenas, Athel Cornish‐Bowden, Paul Fitzpatrick, Peter J. Halling, Vassily Hatzimanikatis, Carsten Kettner, Yanhua Liu, Andrew G. McDonald, Elad Noor, Jürgen Pleiss, Frank M. Raushel, Johann M. Rohwer, Santiago Schnell, Keith F. Tipton, Ming‐Daw Tsai, Urs von Stockar, Ulrike Wittig, Roland Wohlgemuth, John M. Woodley

Date Published: 5th Mar 2026

Publication Type: Journal Article

DOI: 10.1111/febs.70415

Citation: The FEBS Journal 293(11):3111-3126.

Created: 6th Jul 2026 at 20:37, Last updated: 6th Jul 2026 at 20:40

A combined experimental and modelling approach for the Weimberg pathway optimisation

Computational pathway design for biotechnological applications

(Show All)

Abstract (Expand)

The oxidative Weimberg pathway for the five-step pentose degradation to α-ketoglutarate is a key route for sustainable bioconversion of lignocellulosic biomass to added-value products and biofuels. The …

Authors: Lu Shen, Martha Kohlhaas, Junichi Enoki, Roland Meier, Bernhard Schönenberger, Roland Wohlgemuth, Robert Kourist, Felix Niemeyer, David van Niekerk, Christopher Bräsen, Jochen Niemeyer, Jacky Snoep, Bettina Siebers

Date Published: 1st Dec 2020

Publication Type: Not specified

DOI: 10.1038/s41467-020-14830-y

Citation: Nat Commun 11(1) : 82

Created: 3rd Mar 2020 at 10:48, Last updated: 8th Dec 2022 at 17:26

(v.1.18.0)