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Pure cultures of P. cordatum were grown at 20°C, 26°C and 30°C until exponential and stationary growth phase and were harvested for metabolite extraction.
Online metabolite derivatization was performed using a Gerstel MPS2 autosampler (Muehlheim, Germany). Dried metabolites were dissolved in 15 µl of 2 % methoxyamine hydrochloride in pyridine at 40 °C under shaking. After 90 min, an equal volume of N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) was added and held for 30 min at 40 °C. One ...
Creators: None
Submitter: Carsten Reuse