Studies
What is a Study?Short Name: P4_Pcitri_tr1tr2combo Title: Combining tr1 and tr2 P. citri de novo transcriptomes into one transcriptome set Description: Production of one set of transcripts that will be easier for the readers of the planned publication Raw Data: Principal investigator: Špela Baebler License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T31_mealybug
Assays: _A_01_renameFASTA-Bioinfo, _A_02_CDHIT-Bioinfo, _A_03_CDHIT2-Bioinfo, _S_P4_Pcitri_tr1tr2combo-files
Short Name: P4_Pcitri_tr2 Description: Study of P. citri transcriptome data from SUSPHIRE and mealybug.org RNA-Seq datasets Raw Data: Principal investigator: Špela Baebler License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T31_mealybug
Assays: _A_01_assembly2-rnaSPAdes, _A_02_assembly2-IPS, _A_03_mapAs2ToGenome-STAR, _A_04_mapReadsToAs2-STAR, _A_05_As2_tr_limmaDE-R, _S_P4_Pcitri_tr2-files
Short Name: P4_genomes Title: Mealybug genome queries for pheromone biosynthesis genes Description: Survey of the available mealybug genomes for potential sex pheromone biosynthesis genes Raw Data: Principal investigator: Špela Baebler License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T31_mealybug
Assays: _A_IPS-bioinfo, _A_MAST-bioinfo, _A_MEME-bioinfo, _A_TPS_phy-bioinfo, _A_Terzyme-bioinfo, _A_g14825_recon-bioinfo, _S_P4_cand_genomic-files
Short Name: P4_Pcitri_genome Title: P. citri SUSPHIRE samples mapping to genome and differential gene expression analysis Description: Illumina reads from virgin and mated female Pcitri samples were mapped to P. citri genome available at mealybug.org (ensembl) Raw Data: Principal investigator: Ĺ pela Baebler License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T31_mealybug
Assays: _A_01_RNAseq-CLC, _A_02_limma-R, _A_03_limma_min50counts-R, _A_04_limma_min10counts-R, _S_P4_Pcitri_genome-files
Short Name: P4_Pcitri_genome_extSamples Title: P. citri all available samples mapping to genome and differential gene expression analysis Description: Illumina reads from all available P. citri samples (SUSPHIRE, Edinburgh and SRA) were mapped to P. citri genome available at mealybug.org (ensembl) Raw Data: Principal investigator: Ĺ pela Baebler License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T31_mealybug
Assays: _A_01_mapToGenomeAll-STAR, _A_02_mapToGenomeAll_limma-R, _S_P4_Pcitri_genome_extSamples-files
Short Name: P4_Pcitri_IsoSeq Title: P citri Iso-Seq transcriptome generation and search for new sex pheromone biosynthesis genes Description: PacBio IsoSeq transcriptome sequencing and data analysis Raw Data: SRR15093694 Principal investigator: Špela Baebler License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T31_mealybug
Assays: _A_01_NGI-IsoSeq, _A_02_cDNAcupcake-dry, _A_03-IPS, _A_04_mapIllumina-STAR, _A_05_DE_limma-R, _S_P4_Pcitri_IsoSeq-files
Investigation: _I_T3.1_mealybug project: _p_SUSPHIRE Short Name: P4_Pcitri_tr Title: P. citri transcriptome study Description: We are interested in certain mealybugs both because they are important pests and very difficult to control but because their sex pheromones are irregular terpenoids, which are (a) very cool molecules and (b) extremely difficult and expensive to make using chemistry.In our project, we will be doing comparative transcriptomics of virgin and mated females to attempt to ...
Submitter: Marko Petek
Investigation: _I_T31_mealybug
Assays: _A_05_assembly1-rnaSPAdes, _A_06_assembly1-IPS, _A_07_mapAs1ToGenome-STAR, _A_08_mapReadsToAs1-STAR, _A_09_As1_tr_limmaDE-R, _S_P4_Pcitri_tr1-files
Investigation files _I_T31_mealybug
Short Name: P3_expressionEcoli Title: Expression of IDS candidates in E. coli Description: IDS candidate sequences are expressed in E. coli and tested for enzyme activities Raw Data: Principal investigator: Heribert Warzecha License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T31_mealybug
Assays: _A_C98F-EcoliExpr, _A_MeUppS-EcoliExpr, _A_Node14610-EcoliExpr, _A_Node14610DmTP-EcoliExpr, _A_Pc1599-EcoliExpr, _A_Pc23180-EcoliExpr, _A_Pc2704-EcoliExpr, _A_Pc32607-EcoliExpr, _A_Pc7366II-EcoliExpr, _A_PcCis24-EcoliExpr, _A_Trans1153-EcoliExpr, _A_Trans2-EcoliExpr, _A_Trans39-EcoliExpr, _A_Trans57-EcoliExpr, _A_Trans64-EcoliExpr, _A_Trans94-EcoliExpr, _S_P3_expressionEcoli-files
Short Name: P4_AdPathGenes Title: Additional search for genes involved in P citri sex pheromone production pathway Description: Search for additional pathway genes using coexpression etc Raw Data: Principal investigator: Špela Baebler License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T31_mealybug
Assays: _A_01-CoEXpNetViz_tr2-coexpr, _A_02-CoEXpNetViz_gen-coexpr, _S_P4_AdPathGenes-files
Short Name: P4_IDScandidates Title: Generation of the candidate list for cis and trans IDS enzymes from P. citri Description: From the P. citri genome and both de novo transcriptome assembly versions we mined homologues of trans(PF00348, IPR000092) and cis (PF01255, IPR001441) IDS enzymes Raw Data: Principal investigator: Špela Baebler License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T31_mealybug
Assays: _A_IDIsearch-Bioinfo, _A_MSA-bioinfo, _A_OtherMealybugCandidates-Alignment, _A_Strep_CLDS-bioinfo, _A_candidatemining-bioinfo, _A_smallIDSsubunit-motifs, _S_P4_cand_all-files