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| Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
| Data File Title: | samples-publish_PG_D.ELSA.xlsx | ||||||||
| Data File Description: | Batch sample publishing | ||||||||
| Project SEEK ID: | https://fairdomhub.org/projects/222 | ||||||||
| Master metadata sheet guide | |||||||||
| Associated Assay | |||||||||
| Existing Assay SEEK ID: | https://fairdomhub.org/assays/2620 | ||||||||
| OR | |||||||||
| New Assay metadata | |||||||||
| Study SEEK ID: | |||||||||
| Assay Title: | |||||||||
| Assay Description: | |||||||||
| Assay type: | Experimental_assay_type | Powered by: | |||||||
| Technology type: | Technology_type | ||||||||
| SOP SEEK ID: | |||||||||
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| UID | File_PrimaryData | Link_PrimaryData | Checksum_PrimaryData | Name | Scientist | Protocol | Parent | Type | Checksum_PrimaryType | Instrument | Analyte | AnalyteVolume | AnalyteVolumeUnits | Analyte_Vendor | Analyte_Catalog# | AnalyteProcedure | Plate | Plate_Vendor | Plate_Catalog# | PlateWash | PlateBlocker | PlateBlockerProcedure | NegativeControl | NegativeControl_Vendor | NegativeControl_Catalog# | Detector | Detector_Vendor | Detector_Catalog# | ReactionStopper |
| D.ELSA-241202ALT-1 | LAM mAb binding affinity ELISA.xlsx | https://www.immport.org/shared/study/SDY2977 | 979afaf9bc136a952805c4de22f04739 | alpha-LAM Binding Affinity ELISA_PatGrace | Patricia Grace | https://fairdomhub.org/sops/820 | ABP-250314ALT-5 | Competitive ELISA | MD5 | Tecan Infinite M1000pro | lipoarabinomannan (LAM) | 2 | µg/mL | BEI Resources | NR-14848 | overnight at 4°C | ELISA | ThermoFisher | 269620 439454 | PBS Tween 0.5% | 5% BSA | one hour at RT, then washed again with PBST 5 times before adding diluted mAb | Human IgG1 Isotype Control Antibody | Bethyl Laboratory | A80-104P | BD OptEIA™ TMB Substrate Reagent Set | BD | 555214 | 2N H2SO4 |
