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| Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
| Data File Title: | samples-publish_KPRF_D.PCR.xlsx | ||||||||
| Data File Description: | Batch sample publishing | ||||||||
| Project SEEK ID: | https://fairdomhub.org/projects/222 | ||||||||
| Master metadata sheet guide | |||||||||
| Associated Assay | |||||||||
| Existing Assay SEEK ID: | https://fairdomhub.org/assays/2558 | ||||||||
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| New Assay metadata | |||||||||
| Study SEEK ID: | |||||||||
| Assay Title: | |||||||||
| Assay Description: | |||||||||
| Assay type: | Experimental_assay_type | Powered by: | |||||||
| Technology type: | Technology_type | ||||||||
| SOP SEEK ID: | |||||||||
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| UID | File_PrimaryData | Link_PrimaryData | Scientist | Type | Parent | Protocol | ExtractionKit | SynthesisKit | Instrument | NormalizationGene | PrimerSequences | QuantificationMethod |
| D.PCR-241118SAS-1 | 111224_PCR.xlsx | https://fairdomhub.org/data_files/7661 | Ryan Finethy | qRT-PCR | RNA-241112SAS-1; RNA-241112SAS-2; RNA-241112SAS-3; RNA-241112SAS-4; RNA-241112SAS-5; RNA-241112SAS-6; RNA-241112SAS-7; RNA-241112SAS-8; RNA-241112SAS-9; RNA-241112SAS-10; RNA-241112SAS-11; RNA-241112SAS-12; RNA-241112SAS-13; RNA-241112SAS-14; RNA-241112SAS-15; RNA-241112SAS-16; RNA-241112SAS-17; RNA-241112SAS-18; RNA-241112SAS-19; RNA-241112SAS-20 | https://fairdomhub.org/sops/799 | Direct-zol RNA Miniprep kit (Zymo Research) | iScript cDNA synthesis kit (Bio-Rad Laboratories) | Applied Biosystems ViiA 7 Real-Time PCR System | Unspliced XBP1; beta-actin | spliced XBP1 F; 5’-ggtctgctgagtccgcagcagg-3’; R; 5’-gaaagggaggctggtaaggaac-3’ 57; unspliced XBP1 F; 5’-cagactatgtgcacctctgc-3’; R; 5’-cagggtccaacttgtccagaat-3’ 58; HSPA5 F; 5’- acttggggaccacctattcct-3’; R; 5’- atcgccaatcagacgctcc-3’ 59; Trib3 F; 5’- gcaaagcggctgatgtctg-3’; R; 5’-agagtcgtggaatgggtatctg-3’ 60–62; β-actin F; 5’- cgcagccactgtcgagtc-3’; R; 5’-ccttctgacccattcccacc-3’ 63. | delta-delta CT method |
