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| Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
| Data File Title: | samples-publishGFP_D.PCRxlsx | ||||||||
| Data File Description: | Batch sample publishing | ||||||||
| Project SEEK ID: | https://fairdomhub.org/projects/340 | ||||||||
| Master metadata sheet guide | |||||||||
| Associated Assay | |||||||||
| Existing Assay SEEK ID: | https://fairdomhub.org/assays/2441 | ||||||||
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| New Assay metadata | |||||||||
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| Assay type: | Experimental_assay_type | Powered by: | |||||||
| Technology type: | Technology_type | ||||||||
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| UID | File_PrimaryData | Link_PrimaryData | Scientist | Type | Parent | Repository | RepositoryID | Protocol | ExtractionKit | SynthesisKit | PrimerSource | PrimerSequences | Instrument | NormalizationGene | QuantificationMethod |
| D.PCR-240715KAM-1 | MDA 231 EGFP Positive | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM8392373 | Satomi Hirose | Real-time RT-PCR | RNA-240709KAM-1 | Gene Expression Omnibus | GSE272066 | https://fairdomhub.org/sops/741 | RNeasy Plus Mini Kit (74134; Qiagen) | Verso cDNA Synthesis Kit (AB1453A; Thermo Fisher Scientific) | Custom DNA oligonucleotides (Integrated DNA Technologies) | Table S1 | Quantstudio 3 Real-Time PCR System (Thermo Fisher Scientific) | Peptidylprolyl isomerase A (PPIA) | 2-delta delta Ct method |
| D.PCR-240715KAM-2 | MDA 231 EGFP Negative | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM8392374 | Satomi Hirose | Real-time RT-PCR | RNA-240709KAM-2 | Gene Expression Omnibus | GSE272066 | https://fairdomhub.org/sops/741 | RNeasy Plus Mini Kit (74134; Qiagen) | Verso cDNA Synthesis Kit (AB1453A; Thermo Fisher Scientific) | Custom DNA oligonucleotides (Integrated DNA Technologies) | Table S1 | Quantstudio 3 Real-Time PCR System (Thermo Fisher Scientific) | Peptidylprolyl isomerase A (PPIA) | 2-delta delta Ct method |
| D.PCR-240715KAM-3 | MDA 231 Small | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM8392375 | Satomi Hirose | Real-time RT-PCR | CEL-240703KAM-5 | Gene Expression Omnibus | GSE272066 | https://fairdomhub.org/sops/741 | RNeasy Plus Mini Kit (74134; Qiagen) | Verso cDNA Synthesis Kit (AB1453A; Thermo Fisher Scientific) | Custom DNA oligonucleotides (Integrated DNA Technologies) | Table S1 | Quantstudio 3 Real-Time PCR System (Thermo Fisher Scientific) | Peptidylprolyl isomerase A (PPIA) | 2-delta delta Ct method |
| D.PCR-240715KAM-4 | MDA 231 Large | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM8392376 | Satomi Hirose | Real-time RT-PCR | CEL-240703KAM-6 | Gene Expression Omnibus | GSE272066 | https://fairdomhub.org/sops/741 | RNeasy Plus Mini Kit (74134; Qiagen) | Verso cDNA Synthesis Kit (AB1453A; Thermo Fisher Scientific) | Custom DNA oligonucleotides (Integrated DNA Technologies) | Table S1 | Quantstudio 3 Real-Time PCR System (Thermo Fisher Scientific) | Peptidylprolyl isomerase A (PPIA) | 2-delta delta Ct method |
