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Abstract The enzyme Glucose-1-phosphate adenylyltransferase (GlgC, EC: catalyses the first step in glycogen synthesis by converting glucose-1-phosphate into ADP-Glucose, which is added in turn to a growing glycogen chain by glycogen synthases. Thus far, the in vitro study of GlgC were mainly performed using colorimetric or radiolabel-based phosphate release assays, limiting the option for analysing this reaction. With this work, we present a novel in vitro continuous assay coupling the ...

Submitter: Kenric Lee

Investigation: 1 hidden item

Assays: GlgC coupled assay

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