Analysis of the sequences of PGM1 from several Synechocystis strains revealed a different annotation for Synechocystis sp. PCC 6803, which included a 16-amino acid N-terminal extension that is missing in the other strains. Additionally, the experimentally validated transcriptional start site from Synechocystis sp. PCC 6803 suggests a shorter open reading frame for PGM1, with Met 17 as putative translational start site. To clarify if the N-terminal extension is an annotation error, we prepared as recombinant proteins the PGM1 as annotated in Cyanobase (Long-PGM1) and a version without the 16-amino acid N-terminal extension (Short-PGM1).
Views: 119 Downloads: 5
Created: 23rd Mar 2022 at 16:33
Last used: 25th Jun 2022 at 19:22