ExploreAnalysis of the sequences of PGM1 from several Synechocystis strains revealed a different annotation for Synechocystis sp. PCC 6803, which included a 16-amino acid N-terminal extension that is missing in the other strains. Additionally, the experimentally validated transcriptional start site from Synechocystis sp. PCC 6803 suggests a shorter open reading frame for PGM1, with Met 17 as putative translational start site. To clarify if the N-terminal extension is an annotation error, we prepared as recombinant proteins the PGM1 as annotated in Cyanobase (Long-PGM1) and a version without the 16-amino acid N-terminal extension (Short-PGM1).
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Comparison_PGM1_versions.xlsx
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Created: 23rd Mar 2022 at 15:33
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Version 1 (earliest) Created 23rd Mar 2022 at 15:33 by Sofia Doello
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The Deutsche Forschungsgemeinschaft (DFG) is the self-governing organisation for science and research in Germany. It serves all branches of science and the humanities. In organisational terms, the DFG is an association under private law. Its membership consists of German research universities, non-university research institutions, scientific associations and the Academies of Science and the Humanities.
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