Analysis of the sequences of PGM1 from several Synechocystis strains revealed a different annotation for Synechocystis sp. PCC 6803, which included a 16-amino acid N-terminal extension that is missing in the other strains. Additionally, the experimentally validated transcriptional start site from Synechocystis sp. PCC 6803 suggests a shorter open reading frame for PGM1, with Met 17 as putative translational start site. To clarify if the N-terminal extension is an annotation error, we prepared as recombinant proteins the PGM1 as annotated in Cyanobase (Long-PGM1) and a version without the 16-amino acid N-terminal extension (Short-PGM1).
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Created: 23rd Mar 2022 at 15:33
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Version 1 (earliest) Created 23rd Mar 2022 at 15:33 by Sofia Doello
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