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| Metadata | Values (examples) | ||||||||
| Asset Title | Kinetics Pgm1 S63A | ||||||||
| Uploader | Sofia Doello | ||||||||
| Uploader SEEK ID | |||||||||
| Project | SCyCode | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 1466 | ||||||||
| Assay Title | Comparison of PGM1 versions | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | enzymatic activity measurements | ||||||||
| Description | Kinetic characterisation of Pgm1 variants. Expermental data for enzyme reaction rates with increasing concentrations of glucose-1-phosphate | ||||||||
| Experimentalist | Sofia Doello | ||||||||
| Date | |||||||||
| SOP | https://fairdomhub.org/sops/490 | ||||||||
| Publication (optional) | doi: 10.1101/2021.04.15.439997 | ||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | °C | ||||||||
| Start_value (optional) | 30 | ||||||||
| End_value (optional) | 30 | ||||||||
| Comments | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | ||||||||
| Compound (if concentration) | Glucose-1-phosphate | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 3 | ||||||||
| End_value (optional) | 0.0625 | ||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglucomutase 1 S63A | ||||||||
| EC Number | 5.4.2.2 (S63A) | ||||||||
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| Concentration | |||||||||
| Glucose-1-phosphate (mM) | Activity (u/mg) | ||||||||
| (mM) | Long-PGM1 | Short-PGM1 | |||||||
| 3 | 66.20968 | 70.08065 | 66.37097 | 183.3685 | 180.9532 | 182.571 | |||
| 1 | 64.87903 | 64.87903 | 63.14516 | 146.9516 | 140.7581 | 143.5645 | |||
| 0.5 | 48.06452 | 46.65323 | 46.49194 | 105.3387 | 101.8065 | 102.7742 | |||
| 0.25 | 27.98387 | 27.70161 | 28.30645 | 59.95161 | 59.66129 | 57.87097 | |||
| 0.125 | 15.40323 | 15.72581 | 15.32258 | 33.14516 | 33.48387 | 31.93548 | |||
| 0.0625 | 8.064516 | 7.943548 | 7.903226 | 17.03226 | 17.03226 | 16.59677 | |||
