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Figure 2: Model calibration with time-resolved quantitative immunoblot data of mCFU-E cells and BaF3-EpoR cells.

Growth-factor deprived mCFU-E cells (5x106 cells per condition) and BaF3-EpoR cells (1x107 cells per condition) were stimulated with different Epo doses and absolute concentrations were determined for pEpoR (B), pAKT (C), ppERK (D). The scale for pS6 (E) was estimated in arbitrary units. GTP-Ras (F) and ppERK were determined upon stimulation with indicated, color-coded Epo doses. pEpoR was analyzed by immunoprecipitation followed by immunoblotting, GTP-Ras was analyzed after pulldown using a ...

Submitter: Dawie van Niekerk

Investigation: Adlung et al (2017) Molecular Systems Biology

Assays: Mathematical models of the Epo-induced AKT, ERK and S6 activation., Source data for Figure 2: Experimental time-resolved quantitative immuno...

Snapshots: No snapshots

Created: 11th Jan 2017 at 10:33, Last updated: 12th Jan 2017 at 08:02

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