Cell free extract, with Mn and NAD recycling

Conversion of XYL to KG in a cell free extract of Caulobacter crescentus, with 0.15 mM Mn2+ added, and with NAD recycling, metabolites measured enzymatically. https://jjj.bio.vu.nl/models/experiments/shen2020_fig4b/simulate

DOI: 10.15490/fairdomhub.1.assay.993.2

Zenodo URL: None

Created at: 8th Jan 2020 at 15:24

Contents

CFE Mn NADrec data

Data files for the conversion of XYL to KG, in Caulobacter crescentus cell free extract, with NAD recycling, and additional Mn2+ (0.15 mM) added.

cfe-Mn-rec.xlsx

CFE Mn NADrec

Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG in cell free extract. If the Mathematica notebook is downloaded and the data file is downloaded in the same directory, then the notebook can be evaluated, and the figure in the manuscript for the cell free extract with added Mn and NAD rec will be reproduced.

cfe-Mn-rec-SEEK.nb

CFE analysis (shen2)

Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG. Protein levels need to be adapted to CFE levels, see SED-ML scripts

shen2.xml

Fingerprints

These checksums allow you to check a Snapshot you have downloaded hasn't been modified. For details on how to use these please visit this guide

MD5: d4e6111752339537b29081e78ebf4d98

SHA1: ed0043fe9c75dc306c7350466b295ed62ec9b1ec

Citation

Snoep, J., & Shen, L. (2020). Cell free extract, with Mn and NAD recycling. FAIRDOMHub. https://doi.org/10.15490/FAIRDOMHUB.1.ASSAY.993.2

Snapshots

Snapshot 2 (8th Jan 2020) DOI

Snapshot 1 (8th Jan 2020) DOI

Activity

Views: 849 Downloads: 21

Created: 8th Jan 2020 at 15:24

Last updated: 8th Jan 2020 at 15:25

(v.1.16.0)