Cell free extract, with Mn and NAD recycling

Conversion of XYL to KG in a cell free extract of Caulobacter crescentus, with 0.15 mM Mn2+ added, and with NAD recycling, metabolites measured enzymatically.
https://jjj.bio.vu.nl/models/experiments/shen2020_fig4b/simulate

DOI: 10.15490/fairdomhub.1.assay.993.2

Created at: 8th Jan 2020 at 16:24

Contents

CFE Mn NADrec data

Data files for the conversion of XYL to KG, in Caulobacter crescentus cell free extract, with NAD recycling, and additional Mn2+ (0.15 mM) added.

  • cfe-Mn-rec.xlsx

CFE Mn NADrec

Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG in cell free extract. If the Mathematica notebook is downloaded and the data file is downloaded in the same directory, then the notebook can be evaluated, and the figure in the manuscript for the cell free extract with added Mn and NAD rec will be reproduced.

  • cfe-Mn-rec-SEEK.nb

CFE analysis (shen2)

Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG. Protein levels need to be adapted to CFE levels, see SED-ML scripts

  • shen2.xml
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Citation
Snoep, J., & Shen, L. (2020). Cell free extract, with Mn and NAD recycling. FAIRDOMHub. http://doi.org/10.15490/FAIRDOMHUB.1.ASSAY.993.2
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Snapshot 2 (8th Jan 2020) DOI
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Created: 8th Jan 2020 at 16:24

Last updated: 8th Jan 2020 at 16:25

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