Documents

Construct fof TOC1 for comparision with NanoLUC

Binary vector used for transforming with Agro ABI the elf3-2 CCA1p:LUC for rescuing elf3-2 mutation. In this particular case the plants selected were based on hypocotyl length. Based on the data of LUX in which we observed that rhytmicity was rescued in all lines, hypocotyl elongation varied between lines. Therefore, we used hypocotyl length for assesing complementation

Donor plasmid for Gatway cloning

https://benchling.com/s/02xiS4zk?m=slm-xxhynNMU6VkdMdqdfpFr

Donor plasmid for Gatway cloning

https://benchling.com/s/3OtoUun0?m=slm-pL4lmqV9bCWOW3WvoSFL

Binary vector used for transforming with Agro ABI the cca1-1/lhy-1p:LUC for rescuing cca1-1 mutation.

https://benchling.com/s/Zu0nJONs?m=slm-5zxnih8JIOujB9nYIG8E

Binary vector used for transforming with Agro ABI the cca1-11/lhy-1 CCA1p:LUC for rescuing lhy-1 mutation.

https://benchling.com/s/GP25kROo?m=slm-ocCyiEfwuTW5mJsbNQGn

Binary vector used for transforming with Agro ABI the cca1-1/lhy-1p:LUC for rescuing lhy-11 mutation. This was an alternative to NanoLUC and also for testing the behaviour of LHY.

Binary vector used for transforming with Agro ABI the prr9/7-9 CCR2:LUC for rescuing prr7-9 mutation.

Binary vector for transformation with Agro. This construct was intended for comparision with NanoLUC

Binary vector used for transforming with Agro ABI the toc1-2 CCA1p:LUC for rescuing the toc1-2 mutant.

Donor plasmid for Gatway cloning for CCA1 genomic region

https://benchling.com/s/HcUdo0bf?m=slm-v3TyQ8AupdMj0SCqtZ2J

Donor plasmid for Gatway cloning

https://benchling.com/s/PpY2tH3D?m=slm-pRd1S0YhNNVNUhMkT4N1

Donor plasmid for Gatway cloning

https://benchling.com/s/g08AhXBj?m=slm-bGbYOjeh3kiwQQUdokaH

CCA1 genomic fusion with LUC+ for comparision between NanoLUC and Firfly luciferase

https://benchling.com/s/ctB45lUQ?m=slm-ZYj5TlnFsbJeTkd2f9Aa