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T300 (72-96h) +12 ml => split: | |||||||||
time | flask in cm2 | ml inoculum | Hayflick | Extraction sol. | heigth | ||||
2h | F50 | 1 | 5 | 1 | 1.5 | * | |||
6h | T75 | 1 | 20 | 2 | 0.3 | * | |||
12h | T75 | 0.5 | 20 | 2 | 0.28 | ||||
24h | T75 | 0.5 | 30 | 2 | 0.4 | ||||
36h | T75 | 0.5 | 30 | 2 | 0.4 | ||||
48h | T75 | 0.2 | 30 | 2 | 0.4 | ||||
72h | T25 | 0.1 | 10 | 1 | 0.4 | ||||
96h | T25 | 0.1 | 10 | 1 | 0.4 | ||||
* cells are attaching in these first hours | |||||||||
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