Selected Cell
Cell:
Value:
DNA content aorta
Aorta decell.
DMSO in decell
SDS in decell
SDC in decell
Triton in decell
SEM images
mechanical testing
flow rate pig carotid
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| Literature work up for: DNA CONTENT RAT AORTA | NOTE: REDO THIS LIST! IT IS NOT VERY WELL MADE | ||||||||||
| number | Key words in search | Author(s) | Year | Title | Vessel type | Method | Analysis | Result | comment | Accepted? | |
| drying method (if avialble) | |||||||||||
| 1 | rat aorta DNA content | Capron et al. | Growth-promoting effect of diabetes and insulin on arteries an in vivo study of rat aorta | 75.7 ug | no further units for DNAresult | no | |||||
| 2 | rat aorta DNA content | Olivetti et al. | Quantitiative Structural changes of the rat thoracic aorta in early spontaneous Hyertension | 240 ug | |||||||
| 3 | rat aorta DNA content | Evensen & Shepro | 1974 | DNA synthesis in rat aortic endothelium: effect of bacterial Endotoxin and trauma | Rat Aorta | no | rapid autoradiography | H staining | 10 cells/10 mm^2 | no DNAisolation | no |
| 4 | rat aorta DNA content | Berry et al. | 1972 | The growth and development of the rat aorta: changes in nucleic acid and scleroprotein content | liquid nitrogen | shibko et al. | used wet weight only | ||||
| 5 | vessel decellularization and DANN | Li et al. | 2013 | The Fetal Porcine Aorta and Mesenteric Acellular Matrix as Small-caliber Tissue Engineering Vessels and Microvasculature Scaffold | fetal porcine aorta | Proteinase K 37° for 144 hr | spectrophotometry (280 nm) | 271.10 ng/ul | no tissue weight reference | jaein | |
| 6 | vessel decellularization and DANN | Mancuso et al. | 2015 | Decellularized ovine arteries as small-diameter vascular grafts | ovine arteries | PureLink Genomic DNAKit, Invitrogen | PureLink Genomic DNAKit, Invitrogen | spectrophotometry (260 nm) | 150 ng/mg dry weight | used 25 mg dried tissue | jaein |
| 7 | vessel decellularization and DANN | Guler et al. | 2017 | improvement of decellularization efficiency of porcine aorta using dimethyl sulfoxide as penetration enhancer | porcine aorta | freeze dried | freeze dried, Protienase K, Quant-iT | pico green fluorescence (520 nm) | 6900 ng/10 mg dry tissue | about 690 ng/mg tissue | jaein |
| 8 | Liver decell | Coronado et al. | 2017 | Decellularization and Solubilization of Porcine Liver dor Use as Substrate for Porcine Hepatocyte Culture: Method Optimization and Comparison | Porcine LIVER | lypophilization | Quant-iT and Dneasy Blood & tissue kit | pico green fluorescence (520 nm) | 3000 ng/mg dry tissue | PORCINE LIVER | yes |
| 9 | Liver decell | Wang et al. | 2014 | Method for perfusion decellularization of porcine whole liver and kidney for use as scaffold for clinical-scale bioengineering engrafts | Poricne Liver | N/A | Tissue DNAeasy Kit (tiangen Biotech) | N/A | 1000 ng/mg dry weight | PORCINE LIVER | no |
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| Literature work up for: decellularization of aorta | NYE:not yet extracted | |||||||||||||||||||||||||||||||||
| Note: N=native, D=decell | ||||||||||||||||||||||||||||||||||
| number | Key words in search | Author(s) | Year | Title | Tissue type | Method | decell time | enzyme | Dnase I conc. | wash | Analysis | Result | mechanical test | reseeding | comment | Accepted? | link to paper | |||||||||||||||||
| detergents | perf. Or stat. | histology | DNAquant | Corrosion cast | EM/SEM | histology | DNAquant | normalized results (ng/mg dry tissue) | EM/SEM | |||||||||||||||||||||||||
| H&E | EvG | Alcian blue | Sirius red | resorcin fuchsin | ICH | Verhoff-van Gieson | DAPI | Massons tricrhome | Russel movat pentachrome | |||||||||||||||||||||||||
| 1 | Decell rat liver | Hillebrandt et al. | 2015 | Procedure for decellularization of rat livers in an oscillating-pressure perfusion device | rat liver | 1% Triton X-100 and SDS | perfusion | 180 min | yes | no | no | no | no | no | no | no | no | no | yes | yes | no | remaining cell clusters | N: 8000, D:1000 ng/mg dry | no | no | no | no mention of how to dry tissue | yes | papers\068-procedure for decellularization of rat livers in an osicllating-pressure perfusion device.pdf | |||||
| 2 | Decell review | Peter et al. | 2011 | An overview of tissue and whole organ decellularization processes | n/a | explanation of detergents, temperature, enymes, solvents, acids and bases, etc | no | no | no | should be less than 50 ng/mg ECM dry weight | no | no | n/a | n/a | just an overview of decell protocols | yes | papers\007-An overview of tissue and whole organ decellularization.pdf | |||||||||||||||||
| 3 | Decell rat aorta SDS | Grauss et al. | 2003 | decellularization of rat aortic valve allografts reduces leaflet destruction and extracellular matrix remodeling | aortic valve | SDS/ Triton/ Trypsin/ Triton+Trypsin | ? | 24 hr/ 24hr/0.5hr/ 0.5to24hr | Rnase, Dnase | 0.2 mg/ml | HBSS 48 hr | yes | no | yes | yes | yes | yes | no | no | no | no | no | no | no | SDS+ Dnase resulted in best decell and ECM preservation | no | no | no | no | no | no direct comparison of Dnacontent | yes | papers\121087-decellularization of rat aortic valve allografts reduces leaflet destruciton and extracellular matrix remodeling.pdf | |
| 4 | Decell rat aorta SDS | Fitzpatrick et al. | 2010 | effect of decellularization protocol on the mechanical behaviour of porcine descending aorta | porcine descending aorta | Triton+EDTA/SDS/Triton+SDC | static | 24 hr/15hr/24 hr | Rnase, Dnase | 0.2 mg/ml | PBS 72 hr | yes | no | no | no | no | no | no | no | no | no | no | no | no | no image | no | no | no | stress " concentration" | no | no histo shown. No DNAquant | no | papers\121026-effect of decellularization protocol on the mechanical behaviour of porcine descending aorta.pdf | |
| 5 | Aorta SDS | Guler et al. | 2017 | improvement of decellularization efficiency of porcine aorta using dimethyl sulfoxide as a penetration enhancer | porcine aorta | SDS+DMSO/ SDS | static | 1 & 3hr each | Dnase | 200 U/ml | yes | no | no | yes | no | no | yes | yes | no | yes | no | yes | no cells. Structure not ok | N: 690, D:10 ng/mg dry | N: 690, D:10 | disruption of ECM | NYE | NYE | DMSO better w SDS | yes | papers\006-improvement of decellularization efficiency of porcine aorta using dimethyl sulfoxide as a penetration enhancer.pdf | |||
| 6 | Aorta | Hwang et al. | 2010 | The decellularized vascular allograft as an experimental platform for developing a biocompatible small diameter graft conduit in a rat surgical model | rat infrarenal abdominal aorta | HBSS | static | 18hr | Rnase, Dnase | 100 ug/ml | HBSS & PBS 18 hr | yes | yes | no | no | no | yes | no | yes | yes | no | no | no | no | no cells or DNa(DAPI)visible | no | no | no | no | no | no DNAquant | yes | papers\079-the decellularized vascular allograft as an experimental platform for developming a boicompatible small diameter graft conduit in a rat surgical model.pdf | |
| 7 | Aorta | Kohrramirouz et al. | 2014 | Effect of three decellularization protocols on the mechanical behavious and strucural properties of sheep aortic valve conduits | sheep aortic valve | SDS+SDC/Triton+EDTA/Trypsin | ? | 48hr/24hr | Rnase, Dnase | conc. Not mentioned | PBS | yes | no | no | yes | no | no | no | yes | no | yes | no | no | yes | SDS+SDC better. | no | no | no image | mounting uniaxile tensile testing | no | bad histo resolution | yes | papers\048-effect of three decellularization protocols on the mechanical behaviour and structural properties of sheep aortic valve conduits.pdf | |
| 8 | Decell aorta | Fitriatul et al. | 2017 | Evaluation of Recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment | porcine aorta | SDS | ultrasonic treatment | 10hr | no | PBS 5 days | yes | no | no | no | no | no | no | yes | no | no | no | no | yes | no visible DNAremains (DAPI) | no | no | no image | no | vascular SMCs | 6 day reseeding | yes | papers\088-Evaluation of recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment.pdf | ||
| 9 | decell aorta | Hazwani et al. | 2018 | Structural integrity of aortic scaffolds decellularized by sonication deellularization system | porcine aorta | SDS | ultrasonic treatment | 10hr | no | PBS 5 days | yes | no | no | yes | no | no | yes | no | no | no | no | no | no | no visible nuclei. Structure somewhat ok | no | no | no | no | no | semiquant of collagen w histo | yes | papers\089-structural integrity of aortic scaffolds decellularized by sonication decellularization system.pdf | ||
| 10 | decell aorta | syazwani et al. | 2015 | decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering | porcine aorta | SDS | ultrasonic treatment | 24hr | no | no | yes | no | no | no | no | no | no | yes | no | no | yes | no | yes | no visible nuclei. Structure not ok | N: 0.08, D:0.01 ug/mg | N: 80, D:10 | disruption of ECM | compresion relaxation testing | no | DNAnot in ng/mg. Decell longitudinal cuts | yes | papers\090-Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering.pdf | ||
| 11 | decell aorta | Ma et al. | 2017 | development and in vivio validation of tissue-engineered samll diameter vascular grafts from decellularized aortae of fetal pigs and canine vascular endothelial cells | fetal porcine aorta | trypsin | static | 36h | Rnase, Dnase | PBS | yes | no | no | no | no | yes | no | no | no | no | yes | no | yes | no visible nuclei. Structure not ok | no | no | no Ecs left. Strcuture looks better | no | canine vascular Ecs | characterization of Ecs | yes | papers\091-development and in vivo validation of tissue engineered small diameter vascular grafts from decellularized aortae of fetal pigs and canine vascular endothelial cells.pdf | ||
| 12 | decell aorta | negishi et al. | 2017 | evaluation of small diameter vascular grafts reconstructed from decellularized aorta sheets | porcine aora | SDS or triton | static | 15h or 3d | no | sterile water 3d | yes | no | no | no | no | no | no | no | no | no | yes | no | yes | no visible nuclei. Structure ok | N: 5.5, D: 0.5 ug/mg | N: 5500 D: 500 | ECM looks ok | creep testing | no | made from aortic sheets! | yes | papers\092-evaluation of small diameter vascular grafts reconstructed from decellularized aorta sheets.pdf | ||
| 13 | decell aorta | Reid and Callanan | 2019 | hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering | bovine aorta | SDS | static | 36hr | no | deionised water | yes | no | no | yes | no | no | no | no | no | no | yes | no | yes | no visible nuclei. Strucutre not ok. | N: 300 D: 50 ng/mg dry | N: 300 D: 50 | smooth fiber structure | instron tensile tester | HUVECs | in the graphs DNa seems to increase | yes | papers\093-hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering.pdf | ||
| 14 | decell aorta | Liu et al. | 2019 | an innovative method to obtain porous porcine aorta scaffolds for tissue engineering | porcine aorta | SDS | vacuum freezing | n/a | no | n/a | yes | yes | no | yes | no | yes | no | no | no | no | yes | no | no | no visble nuclei. Structure not ok | N: 1.5 D: 0.5 fold changes | fibers disrputed | tensile tester | human MSCs | weird DNAvalues | yes | papers\094-an innovative to obtain porous porcine aorta scaffolds for tissue engineering.pdf | |||
| 15 | decell aorta | lin et al. | 2019 | decellularized porcine coronary artery with adipose stem cells for vascular tissue engineering | porcine coronary arteries | Triton | static | 3d | no | 3x w PBS 30min | yes | no | no | no | no | no | no | yes | no | no | yes | no | no | no nuclei. Structure not ok | N: ? D: 20 % | no Dnaunits | no | no | rat adipose Stem cells | no native DNAvalue | no | papers\095-decellularized porcine coronary artery with adipose stem cells for vascular tissue engineering.pdf | ||
| 16 | decell aorta | Aldrige et al. | 2017 | Development and characterisation of a large diameter decellularised vascular allograft | human aorta | Tris then SDS | not stated | 14d | no | PBS several days | yes | no | yes | yes | no | yes | no | yes | no | no | yes | no | no | nuclei present. Revised decell:no nuclei | N: 88 D: 5 ng/mg dry tissue | N: 88 D: 5 | no | uniaxial tensile testing | A549 or BHk cells | low native DNAvalues | yes | papers\096-Development and characterisation of a large diameter decellularised vascular allograft.pdf | ||
| 17 | DnaContent aorta | Weymann et al. | 2014 | Total Aortic Arch Replacement: Superior Ventriculo-Arterial coupling with decellularized allografts compared with conventional prostheses | canine aortic arches | SDS and NaN3 | static | 48 hr | no | PBS 12hr | yes | no | no | no | no | no | no | no | yes | yes | yes | no | yes | no visible nuclei. Structure not ok | N: 500 D: 20 ng/mg dry tissue | N: 500 D: 20 | Retainment of collagen fibrils | static material testing | no | yes | papers\065-total aortic arch replacement superior ventriculo-arterial coupling with decellularized allografts compared with conventional prostheses.pdf | |||
| 18 | Decell rat | Simsa et al. | 2018 | Systematic in vitro comparison of decellularization protocols for blood vessels | porcine vena cava | comparison of diff protocols | perfusion | 2-19 hr | Dnase | 40 U/ml | 7-30hr | yes | no | yes | no | no | yes | yes | no | yes | no | yes | no | no | no images | N: ? D: 0.5 ng/mg | N: ? D: 0.5 ng/mg | no | youngs modulus, burst pressure, tensile strength | HUVECs | no histo images shown! Only DAPI | yes | papers\097-Systematic in vitro comparison of decellularization protocols for blood vessels.pdf | |
| 19 | decell rat | sesli et al. | 2018 | decellularization of rat adipose tissue, diaphragm, and heart: a comparison of two decellularization methods | rat adipose tissue, diaphragm and heart | SDS and freeze thaw | static | 2d and 1 w | no | PBS overnight | yes | no | no | no | no | yes | no | no | no | no | no | no | no | images too dark | no | no | no | no | AdMSCs | histo too dark. No DNAqaunt. No mechanical testing | no | papers\098-Decellularization of rat adipose tissue, diaphragm, and heart a comparison of two decellularization methods.pdf | ||
| 20 | decell aorta | fercana et al. | 2014 | platform technologies for decellularization, tunic specific cell seeding, and in vitro conditioning of extended length, small diameter vascular grafts. | bovine femoral arteries | SDS | perfusion | 12 days | Dnase | 720 mU/mL | 70%EtOH, ddH2O, PBS | yes | no | no | no | no | yes | yes | yes | no | no | yes | no | no | no visible nuclei. Structure ok | N:1500 ng/mg D:150 ng/mg | N:1500 ng/mg D:150 ng/mg | no | burst pressure, ring open angle, STR, diametrical compliance | HUVECs | bioreactor setup is good idea | yes | papers\121100-platform technologies for decellularization, tunic-specific cell seeding, and in vitro conditioning of extended length, small diameter vascular grafts.pdf | |
| 21 | aorta decell | jiang et al. | 2015 | a polymer extracellular matrix compostie with improved thromboresistance and decellularization properties | rat aorta | Triton x-100 + SDS | perfusion | 4 days | Dnase | 100 or 400 U/mL | ddH2O | yes | no | no | no | no | yes | no | yes | yes | no | yes | no | yes | no visible nuclei. Structure ok. | N:100% D:5% | not availbale | Retainment of collagen fibrils | stress strain resistance | HUVECs and HASMCs | fabrication of polymer ECM | yes | papers\121101-A polymer-extracellular matrix composite with improved thromoresistance and recellularization properties.pdf | |
| 22 | decell aorta | Pennel et al. | 2014 | the performance of cross linked acellular arterial scaffolds as vascular grafts; preclinical testing in direct and isolation loop circulatory models | porcine renal arteries | 1M NaOH | not stated | 3 hours | no | ddH2O then PBS | yes | no | no | no | no | no | yes | yes | yes | no | yes | no | yes | cannot see | N:30ug/mg wet D:2.5ug/mg wet | N:30000 ng/mg wet D:2500 ng/mg wet | only shown after reexplantation. No native or decell to compare results to | STR, burst pressure, diamtetrical compliance | in vivo | histo images too small | no | papers\121102-the performance of cross-linked acellular arterial scaffolds as vascular grafts preclinical testing in direct and isolation loop circulatory models.pdf | ||
| 23 | decell aorta | jeoung et al. | 2013 | the effect of space fillers in the cross linking processes of bioprotheses | bovine pericardia | ddH2O, Tris, SDS, Triton | not stated | 2 days | no | PBS | yes | no | no | no | no | yes | no | yes | no | no | no | no | no | no H&E of decell aorta | no | no | no | tensile strenght and elasticity | aortic SMCs | no DNAquant | no | papers\121103-The effect of space fillers in the cross-linking processes of bioprosthesis.pdf | ||
| 24 | decell vessel | bertanha et al. | 2014 | tissue engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors | rabbit vena cava | 1% SDS | perf | 2 h | no | PBS | no | no | no | no | no | no | no | no | yes | no | no | no | no | no visible nuclei, structure ok, but visibly altered | no | no | no | no | yes MSC | used vena cava | no | |||
| 25 | decell vessel | böer et al | 2011 | The effect of detergent based decellularization procedures on cellular proteins and immunogenicity in equine carotid artery grafts | equine carotid arteries | 0.5% SDS and SDC | not stated | 36-42 h | benzanoase | 125 U/mL | VE water | yes | no | no | no | no | no | no | no | no | no | yes | no | no | no visbible nuclei, strucutre not ok | N: 191.2+- 20.14 ng D: 0.60+-15 ng dry tissue | no | no | no | no | detection of MHC class 1 | no | ||
| 26 | decell vessel | cheng et al. | 2019 | Combination of freeze thaw with detergents: a promising approach to the decellularization of porcine carotid arteries | porcine carotid arteries | 1% Triton X-100+- freeze thaw | stat | 6 days | trypsin | PBS | yes | no | yes | no | no | no | yes | yes | yes | no | no | no | sem | unclear HE, images too small, DAPI unclear | no | no | ice crystals visible, collagen structure visible, cellular structures visible | no | no | unclear results | no | |||
| 27 | decell vessel | dahl et al. | 2003 | decellularized native and engineered arterial scaffolds for transplantation | porcine carotid arteries | 1% Triton X 100 | not stated | 24 h | Dnase 1 | 0.2 mg/ml | PBS | yes | no | no | no | no | no | yes | no | yes | no | yes | no | no | blurry images | unclear whether wet or dry weight used. Unclear ng quantification | no | no | burst pressure | qno | no: vessel too big | |||
| 28 | decell vessel | daugs et al. | 2017 | detergent based decellularization of bovine carotid arteries for vascular tissue engineering | bovine carotid arteries | detergent (SDS or TX100) DOA, 0.1 M Tris-HCLm CHAPS, PBS, NaCl, EDTA | perf | 38 h | no | no | unclear | yes | no | no | no | no | no | no | no | no | yes | yes | no | no | some nuclear smudge present, structure of scaffold visibly different from native | unclear: results shown in percentages but no comparison to native | no | no | tensile test | no | scaffold does not seem nuclear content free. Vessel too big | no | ||
| 29 | decell vessel | fooladi et al. | 2022 | an efficient strategy to recellularization of a rat scaffold: an optimized decellularization detergent removal and apelin 13 immbilozation | rat aorta | TritonX-100 SDS CHAPS peracetic acid | perf | 18 h | no | no | yes but no mention of what was used | yes | no | no | no | no | no | no | no | yes | no | yes | no | FESEM | no visible nuclei, structure is kind of ok | N: 8, D: 2 | unclear | unclear | no | yes HUVECS | results are kind of ok | yes | ||
| 30 | decell vessel | Grandi et al. | 2011 | decellularized bovine reinforced vessels for small diameter tissue engineered vascular grafts | bovine tibial arteries and veins | SDS | static | 78 h | DNAse I | PBS | yes | yes | yes | yes | no | yes | images too small, presence of nuclear smudge visible in DAPI stain | unclear, results say "cell number" rather than Dnaconcentration | no | structure visibly changed | tensile test | no | no | no | ||||||||||
| 31 | decell vessel | ketchedjian et al. | 2005 | recellularization of decellularized allograft scaffolds in ovine great vessel reconstructzions | sheep pulmonary conduits | NLS | perf | 36 h | benzanoase | 24-36 h | yes | no | yes | no | no | yes | no | no | no | yes | yes | no | no presence of nuclear content in HE, but no DAPI stain | 99.8% reduction | no | no | no | yes via implantation of vessel | calcification | no | ||||
| 32 | decell vessel | Li et al. | 2013 | the fetal porcine aorta and mesenteric acellular matrix as small caliber tissue engineering vessels and microvasculature scaffold | fetal porcine aorta | SDS+ Triton X 100 | static | 26 h | no | no | PBS | yes | no | no | no | no | no | no | yes | yes | no | yes | no | yes | no visible nuclei, structure ok | N: 277 D: 3.12 ng/ul DANN | no | no | yes via implantation of the vessel | paracetic acid | yes | |||
| 33 | decell vessel | Mallis et al. | 2018 | recellularization potential of small diameter vascular grafts derived from human umbilical artery | human umbilical artery | CHAPS+EDTA+SDS | unclear | 22 h | no | no | PBS | yes | no | yes | no | no | no | no | yes | yes | no | yes | no | no | no visible nuclei, structure ok | N:1500 D:500 ng/tissue weight (mg) | no | no | no | tried static and perf | recellularization | yes | ||
| 34 | decell vessel | mancuso et al. | 2014 | decellularized ovine arteries as small diameter vascular grafts | ovine arteries | 1% SDS, Trypsin, 1% TritonX100 | static | 60 h | dnase | 1.2 mg/ml | PBS | yes | yes | no | no | no | yes | no | no | yes | no | yes | no | yes | no visible nuclei but images too small, struture ok | N:150 ng/mg D:10 ng /mg dry tissue | no | ultrastructure semilngly unchaged, rather damaged | no | human MSCs | no | no | ||
| 35 | decell vessel | mangold et al | 2012 | evaluation of decellularized human umbilical vein for vascular tissue engineering-comparison with endothelium denuded HUV | human umbilical vein | 0.05% TritonX100 and SDCm IGEPAL-CA630 | static | unclear, but more than 12 h | dnase | 100 ug/ml | PBS | yes | yes | yes | yes | no | yes | no apparent removal of nuclear content without DNAse treatment, with dnase treatment removal of nuclear content and obvious change in morphology | unclear | no | ultrastzructure different, exposed collagen? | tensile testing | HUVECs | no | no | |||||||||
| 36 | decell vessel | pellegata et al | 2013 | detergent enzymatic decellularization of swine blood vessels insight on mechanical properties for vascular tissue engineering | porcine abdominal aorta | SDC | static | 79 h | Dnase | 2000 kU | PBS | yes | no | no | no | no | no | no | yes | no | no | yes | no | yes | no visible nuclei, but nuclear smudge visible, structure ok | N: 2500, D: 500 ng/mg | no | ultrastructure changed, collahen fibers exposed | uniaxial tensile testing | no | no | |||
| 37 | decell vessel | Pu et al | 2018 | determining the optimal protocol for preparing an acellular scaffold of tissue engineered small diameter blood vessels | porcine carotid arteries | SDS, SDC, TritonX100 | static | 60 h | no | no | PBS | yes | no | no | no | no | no | no | no | no | yes | yes | no | yes | image too small | N: 600 ng/mg dry weight D: unclear | no | fibers visible, but also other structures? | ultimate tensile strain and stress strain testing | no | no | no | ||
| 38 | decell vessel | Rambol et al. | 2018 | Recellularization of decellularized venous grafts using peripheral blood a critical evaluation | human cadaver femoral vein | 1% SDS and TnBP | static | 28 h | Dnase | 4 mg/L | EtOH, PBS | yes | yes | yes | yes | yes | nucleic content visible in DAPI | unclear | unclear | ultrastructure different, collagen exposed | no | perfusion blood, structure of recellularized vessel not ok | less nuclei visible in recellularized vessel with DAPI | no | ||||||||||
| 39 | decell vessel | Rodriguez-Rodriguez et al. | 2018 | human umbilical vessels: choosing the optimal decellularizatio method | human umbilical vein | SDS, CHAPS | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | unclear | yes, compliance | no | no | |||
| 40 | decell vessel | seiffert et al | 2021 | in vitro recellularization of dcecellularized bovine carotid arteries using human endothelial colony forming cells | bovine carotid arteries | Trypsin, TritonX100 | static | 56 h | dnase 1 | 8 mg/ml | ethanol and peracetic acid | yes | no | no | no | no | yes | no | yes | no | no | yes | no | no | structure wierd, no visible nuclei but image too small. DAPI shows nucleic remnants | N: 2000 ng/mg D: 100 ng/mg dry weight | no | no | ultimate tensile strain and stress strain testing | ECFC | no | no | ||
| 41 | decell vessel | Teebken et al. | 2009 | human iliac vein replacement with a tissue engineered graft | caval vein | SDS | static | 90 min | dnase1 | unclear | PBS | yes | no | no | no | no | no | no | no | no | no | no | no | no | images blurry. Results unclear | no | no | |||||||
| 42 | decell vessel | tuan-mu et al | 2020 | removal of an abluminal lining improves decellularization of human umbilical arteries | human umbilical arteries | SDS | perf | unclear | unclear | unclear | VE water | yes | no | yes | yes | no | yes | no | no | no | no | yes | no | yes | nuclear smudge present | unclear | no | unclear | pressure burst | no | no | no | ||
| 43 | decell vessel | Xiong et al | 2013 | decellularized porcine saphenous artery for small diameter tissue engineered conduit graft | porcine saphenous artery | VE Water, Trypsin, NG4OH | static | 6 days | no | no | fetal bovine serum | yes | no | no | no | no | no | n o | yes | yes | no | no | no | yes | no visible nuclei in native nor decell. Images too small, structure not ok, DAPI shows remnant nucleic smudge | no | no | structure visibly changed, not ok | no | yes via implantation | no | no | ||
| 44 | vessel decellularisation | O connor et al | 2016 | on the automatic decellularusation of porcine aortae a repeatability study using a non enzymatic approach | porcine aortae | 2% Triton and SDC | static | 2 days | Rnase | no | PBS, VE water | no | no | no | no | no | yes | no | no | no | no | yes | no | no | no HE present. Images too small to see. Nuclei present in the laminin stain | N: 220-351 ng/mg wet weight D: 165-249 ng/mg wet weight | yes | uniaxial tensile testing | no | decell does not seem complete | no | https://www.thno.org/v12p4684.htm | ||
| 45 | vessel decellularisation | Umashankar et al | 2016 | long term healing of mildly corss linked decellularized bovine pericardial aortic patch | bovine pericardium | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | yes | no | no | no | no | no | no | no | yes | no | no | no | no | no results shown from decellularized tissue | no | no | no | incomplete decell information | no | https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33755 | |||
| 46 | vessel decellularisation | Harper et al | 2020 | portal venous repopulation of decellularised rat liver scaffolds with syngeneic bone marrow stem cells | rat liver | 1%Truton NH4OH in PBS | perfusion | 6 hours | no | no | distilled water | yes | no | no | no | no | no | no | no | no | no | yes | no | yes | cell free | D: 10 ng/mg lyophilised scaffold | yes | no | stem cells | focus on liver | no | |||
| 47 | vessel decellularisation | Bond et al. | 2022 | development and preliminary testing of porcine blood derived endothelial like cells for vascular tissue engineering applications; protocol optimisation and seeding of decellularised human saphenous veins | saphenous veins | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | focus on cells | no | ||||||
| 48 | vessel decellularisation | Hoegsted Ahlmann et al. | 2021 | decellularised human umbilical artery as a vascular graft elicit minimal pro inflammatory host response ex vivo and in vivo | human umbilical arteries | 4% SDC, 1% Triton | static | 2.5 days | Dnase | 4 mg/L | PBS and NaN3 | yes | yes | no | no | no | no | no | yes | no | no | no | no | no | Histological staining validated efficient elimination of cell components and nuclei | no | no | no | no | implantation | yes | https://pmc.ncbi.nlm.nih.gov/articles/PMC8347020/#sec2-ijms-22-07981 | ||
| 49 | vessel decellularisation | Tornifoglio et al. | 2020 | Diffusion tensor and arterial tissue: establishing the influence of arterial tissue microstructure on fractional anisotropy, mean diffusivity and tractography. | porcine carotid arteries | 0.1M NaOH, 0.1M NaCL | perf | 32 h | Dnase | 10 ul/ml | no | yes | no | yes | yes | no | no | yes | no | no | no | no | no | no | no visible nuclei but no DAPI nor DNAquant to confirm | no | no | no | tractography | no | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC7693170/#Sec2 | |
| 50 | vessel decellularuzation | Massaro et al. | 2022 | Decellularization of porcine carotid arteries from the vessel to the high quality scaffold in five hours | porcine carotid arteries | 1% Triton and 1% SDS | perf | 5 h | no | no | saline water | yes | yes | no | no | no | yes | no | yes | no | no | no | no | no | no visible nuclei also in DAPI | no | no | no | uniaxial tensile testing | also SDS resdidue assay | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC9150822/#s2 | ||
| 51 | vessel decellularization | Dahan et al. | 2023 | dynamic autologous reendothelialization of small caliber arterial extracellular matrix a preclinical large animal study | porcine carotid arteries | NaCL, Triton, Trypsin NH4OH | static | 5 days | no | no | EtOH, PBS | no | no | no | no | no | yes | no | no | yes | no | no | no | yes | no visible nuclei but images too small | no | no | unclear | burst pressure | by implantation | biocompatibility tests | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC5240014/#s015 | |
| 52 | vessel decellularization | Lopez-Ruiz et al. | 2017 | polyethylmethacrylate co diethylaminoethyl acrylate coating improves endothelial repopulation biomechanical and anti thrombogenic properties of decellularized carotid arteries for blood vessel replacement. | porcine carotid arteries | Ve water, 0.05% Triton and ammonium hydroxide | static | 6 days | trypsin | no | VE water | yes | no | no | no | no | no | no | yes | yes | no | no | no | yes | no visible nuclei in HE, some blue smudge in DAPI and ECM disrupted | no | no | visible cells | ultimate tensile strength and burst pressure | yes in cell culture | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC5412652/#Sec2 | |
| 53 | vessel decellularization | Xu et al. | 2017 | preparation and charact erization of small diameter decellularized scaffolds for vascualr tissue engineering in an animal model | rabbit carotid arteries | EDTA VE water 1% Triton | static | up to 4 hours? Unclear | yes | 70 U/ml | yes but no mention of what was used | yes | no | no | yes | no | no | no | yes | yes | no | no | no | yes | HE looks clear, but DAPI shows high presence of nuclear content | no | no | ECM disrupted | suture retention strength with 5-0 poluprpelyne 1 mm, burst pressure | cytotoxicity assay MTS | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC5425976/#Sec2 | |
| 54 | vessel decellularization | Wang et al. | 2021 | Photoxidation crosslinking to recover residual stress in decellularized blood vessel | rat carotid artery | VE water, EtOH PBS freeze thaw | static | 2 days | trypsin | no | PBS | yes | no | no | no | no | no | no | no | yes | no | no | no | yes | nuclear smudge present | no | no | difference between native and decell is unclear | angle opening | crosslinking MB-UV2h | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC7955719/#sec2 | |
| 55 | vessel decellularization | Hakansson | 2021 | Individualized tissue engineered veins as vascular grafts a proof of concept stufy in pig | porcine vena cava | Triton Trinbutylphosphate | perf | 7 days | Dnase | 40 U/ml | PBS | yes | no | no | no | no | yes | no | yes | no | no | yes | yes | no visible nuclei | 5 ng/mg wet tissue | no | difference unclear | no | yes | no | no | https://onlinelibrary.wiley.com/doi/10.1002/term.3233 | ||
| 56 | vessel decellularization | Jenndahl | 2022 | personalized tissue engineered arteries as vascular graft transplants a safety study in sheep | sheep carotid arteries | freeze thaw Triton TnBP | static | 9 days | Dnase | 40 U/ml | PBS | yes | no | no | no | no | no | yes | yes | no | no | yes | no | yes | no visbible nuclei | 0.3 ng/mg wet tissue | no | clear difference | ring tensile test, burst pressure, failure strain, stiffness | yes and implantation- hyperplasia | yes | https://pmc.ncbi.nlm.nih.gov/articles/PMC9463533/#sec2 | ||
| 57 | vessel decellularization | omid et al. | 2023 | biomimetic vascular tissue engineering by decellularized scaffold and concurrent cyclic tensile and shear stresses | ovine coronary arteries | NaCl and VE water, Trypsin, Triton X | static | 9 days | trypsin | no | PBS | yes | no | no | no | no | no | no | no | yes | no | no | no | yes | no visible nuclei but ECM not ok | no | no | repopulation clearly shown | tensile test | MTT assay | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC10014704/#Sec2 | |
| 58 | vessel decellularization | Liu et al. | 2022 | photooxidation and pentagalloyl glucose cross linking improves the performance of decellularized small diameter vascular xenograft in vivo. | bovine internal mammary artery | SDS Triton | sonication and perfusion | 4 days | Dnase Rnase | 40 U/ml | PBS | yes | yes | no | no | no | yes | no | no | yes | no | yes | no | yes | smudge of nuclei present | 50 ng/mg wet weight | no | differences unclear | burst pressure and suture retention 6-0 prolene and 2 mm on INSTRON system | yes with cell ine and MTT assay | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC8987116/#s2 | |
| 59 | vessel decellularization | Falkner et al. | 2023 | Acellular human placenta small diameter vessesl as a fabourable source of super microsurgical vascular replacements a proof of concept | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | not mentioned | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC10045636/#sec3-bioengineering-10-00337 | ||
| 60 | vessel decellularization | Liu et al. | 2017 | cytocompatibility and biologic characteristics of synthetic scaffold materials of rabbit acellular vascular matrix combining with human like collagen 1 | rabbit arteries | Triton | static | 3 days | no | no | PBS | yes | no | no | no | no | no | no | no | yes | no | no | no | yes | some nuclear smudge present | no | no | difference unclear | breaking strength and elongation, burst pressure | in vitro cytotoxicity and seeding with hunman fibroblasts | no | https://journals.sagepub.com/doi/10.1177/0885328217728448?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed#sec-2 | ||
| 61 | vessel decellularization | Kong et al. | 2018 | the use of heparin bGFG and VEGF 145 grafted acellular vascular scaffold in small diameter vascular graft | rabbit abdominal aorta | Triton Hyclone | static | 3 days | DNASE Rnase | 0.2 mg/ml | PBS | no | no | no | no | no | yes | no | no | no | no | no | no | yes | no | no | no | some difference visible in decell structure | burst pressure and suture strenght 5-0 nylon and 2 mm | implantation | no | no | https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.34160 | |
| 62 | vessel decellularization | Gu et al. | 2018 | preparation and evaluation of decellularized porcine carotid arteries cross linked by genipin the preliminary results | porcine carotid arteries | trypsin EDTA | static | 2 days | n o | no | VE water | yes | yes | no | no | no | no | no | no | yes | no | no | yes | nuclear smudge present | no | no | difference clear | stress strain test suture strenght and burst pressure with 5-0 prolene and 2 mm | no | https://link.springer.com/article/10.1007/s10561-017-9675-9#Sec2 | ||||
| 63 | vessel decellularization | Du et al. | 2022 | Gelatin sodium alginate hydrogel coated decellularized porcine coronary artery to construct bilayer tissue engineered blood vessels | porcine carotid arteries | SDS Triton | static | 9 days | no | no | PBS | yes | no | no | no | no | no | no | no | yes | no | yes | no | yes | nuclear smudge present | unclear | no | difference unclear | stress strain test | cell culture mouse fibroblasts | no | no | https://www.sciencedirect.com/science/article/pii/S0141813022009023?via%3Dihub#s0010 | |
| 64 | vessel decellularization | cheng et al. | 2023 | development of heparinized and hepatocyte growth factor coated acellular scaffolds using porcine carotid arteries | porcine carotid arteries | Tris, EDTA, SDS, Triton | static | 4 days | no | no | VE water | yes | yes | no | no | no | no | no | yes | yes | no | yes | no | yes | looks free of nuclear content | 0 ng /mg Dry weight | clear difference | uniaxial tensile testing suture retention with 5-0 prolene 1 mm, burst pressure | rat mesenchymal stem cells | no | no | https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.35317 | ||
| 65 | vessel decellularization | lin et al. | 2021 | sonication assisted method for decellularization of human umbilical artery for small caliber vascualr tissue engineering | human umbilical arteries | SDS | sonication | 2-8 h | no | no | PBS | yes | yes | no | no | no | yes | no | yes | yes | no | yes | no | yes | nuclear smudge present | 10 ng/mf Dry weight | no | clear difference | maximum pressure | cell seeding with human umbilical vein endothelial cells | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC8196986/#sec2-polymers-13-01699 | |
| 66 | vessel decellularization | Chen et al. | 2018 | the promotion of tissue engineering blood vessel patency by CGS21680 through regulating pro inflammatory activitis of endothelial progentior cell | rat common carotid artery | trypsin | static | 40 min | Rnase Dnase lipase | not mentioned | PBS | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | no | human peripheral blood | no | https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.36457 | ||
| 67 | vessel decellularization | Muniswasmi et al. | 2020 | Endothelial progenitor stem cells in engineered vessels for vascular transplantation | human umbilical cord artery | Triton NaCl SDS | static | 3 days | no | not mentioned | not mentioned | yes | no | no | no | no | no | no | yes | no | no | yes | no | yes | nuclear smudge present | 10 ng/mg | no | clear difference visible | uniaxial test | human stem cells | no | no | https://link.springer.com/article/10.1007/s10856-020-06458-7/figures/4 | |
| 68 | vessel decellularization | Lehmann et al. | 2017 | EDC cross linking of decellularized tissue a promising approach? | porcine aortic wall tissue | SDS | no access | about 2 days | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no access | no | https://www.liebertpub.com/doi/10.1089/ten.tea.2016.0416 | |
| 69 | vessel decellularization | Messner et al | 2023 | cyclic pressure induced decellularization of porcine ascending aortas | porcine ascending aortas | SDC, SDS | perfusion | 24 h | no | no | no | yes | no | no | no | no | no | no | yes | no | no | yes | no | yes | abundant nuclei present | 0.7 ug/ml/mg tissue | no | differences unclear | yes but no details | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC10115674/#Sec2 | ||
| 70 | vessel decellularization | Yamanaka et al. | 2020 | decellularization of submillimeter diameter vascular scaffolds using peracetic acid | rat tail arteries | PAA EtOH, VE water, u | perf vs static | 1-3days | Dnase | 40 U/ml | saline water | yes | no | no | no | no | no | no | no | no | no | no | no | no | nuclei present and scaffold not ok | no | no | no | no | implantation | no | no | https://link.springer.com/article/10.1007/s10047-019-01152-0#Sec2 | |
| 71 | vessel decellularization | Eyre et al. | 2020 | re endothelialization of non detergent decellularized porcien vessel | porcine carotid arteries and mammaria interna | free thaw NaOH NaCl VE water | perf vs static | 2 days | Dnase | 300 U/mL | PBS | yes | no | no | no | no | no | no | yes | no | yes | yes | no | yes | nuclei present in static decell, not visible in perfusion decell | 10 ug/mg dry weight | difference unclear | burst pressure analysis | with HUVECS and Human cardiac endothelial cells | no | no | https://onlinelibrary.wiley.com/doi/10.1111/aor.13836 | ||
| 72 | vessel decellularization | Kumar et al. | 2018 | decellularization and recellularization methodology for human saphenous vein | human saphenous vein | Triton tnbp | perf | 5 days | Dnase | 40 U/ml | PBS | yes | no | no | no | no | no | no | no | no | no | no | no | no | no visible nuclei | no | no | no | no | yes with endothelial cells | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC6126553/#sec2 | |
| 73 | vessel decellularization | Chen et al. | 2020 | decellularization of porcine carotid arteries using low concentration sodium dodecyl sulfate | porcine carotid arteries | Trusm EDTA Triton, SDS for 30-72 hours | freeze thaw | from 30-72 h | no | no | PBS | yes | yes | yes | no | no | yes | no | yes | yes | no | yes | yes | no visible nuclei | from 100-200 ng/mg dry weight | clear difference | uniaxial tensile testing, burst pressure, suture strength with 5-0 at 1 mm | implantation | no | no | https://journals.sagepub.com/doi/10.1177/0391398820975420?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed#sec-2 | |||
| 74 | vessel decellularization | simsa et al. | 2019 | effect on fluid dynamics on decellularization efficacy and mechanical properties of blood vessels | porcine vena cava | Triton TnBP | static vs perfusion | 3 days | Dnase | 40 U/ml | PBS | yes | no | no | no | no | yes | no | yes | no | no | yes | no | yes | no visible nuclei, maybe nuclear smudge present in some results | N: 500 ng/mg tissue D: 0.3 ng/mg tissue. Unclear if wet or dry tissue | clear difference | uniaxial testing burst pressure | MTT assay HUVECS | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC6682308/#sec002 | ||
| 75 | vessel decellularization | kostelnik et al. | 2021 | small diameter artery decellularization effects of anionic detergent concentration and treatment duration on porcine internal thoracic arteries | porcine thoracic aorta | SDS and SDC | static | from 24-72 h | Dnase | 1 mg/ml | unclear | no | no | no | yes | no | no | yes | yes | no | no | yes | no | yes | only 72 h reduced DAPI signal | only 72 reduced DNAqu<ant | clear difference | biacial mechanical test | no | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC8854343/#S2 | ||
| 76 | vessel decellularization | Meiring et al. | 2017 | tissue engineered small vessel conduits the antithrombotic effect of re endothelialization of decellularized baboon arteries a preliminary experimental study | baboon femoral, carotid, radial arteries | sodium deocycholic acid, SDS and triton | unclear | unclear | no | no | PBS | no | no | no | no | no | no | no | no | no | no | no | no | yes | no | no | no | unclear | no | HUVECS and MTT assay | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC5674964/#sec6 | |
| 77 | vessel decellularization | Koenig et al. | 2018 | successful decellularization of thick walled tissue highlighting pitfalls and the need for multifactorial approach | porcine aorta | NaCl SDC SDS | static vs perfusion | from 24-72 h | Dnase | 30 U/ml | NaCl | yes | no | no | yes | no | yes | no | yes | no | yes | yes | no | yes | reduction is clear in scaffolds decelled for 72 h | N:0.5 ug/mg tissue D: 0,1 ug/mg tissue but it is unclear whether wet or dry tissue weight | clear difference | no | no | no | https://journals.sagepub.com/doi/10.1177/0391398818805624?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed#sec-2 | |||
| 78 | vessel decellularization | negishi et al. | 2012 | porcine radial artery decellularization by high hydrostatic pressure | porcine radial artery | pressure | static | 7 days | Dnase | 0.2 mg/ml | saline water | yes | yes | no | no | yes | yes | no | no | no | no | yes | yes | no visible nuclei | N:6 ug/mg D:1ug/mg but unclear whether wet or dry tissue | difference unclear | suture retention test 5-0 silk and 3mm | implantation | no | no | https://onlinelibrary.wiley.com/doi/10.1002/term.1662 | |||
| 79 | vessel decellularization | Ishino and Fujisato | 2015 | decellularization of porcine carotid by recipients serum and evaluation of ist biocompatibility using a rat autograft model | porcine carotid artery | human or rat serum vs SDS | static | 1-5 days | no | no | unclear | yes | no | no | no | no | no | no | no | no | no | yes | no | yes | no visible nuclei after 5 days | N: 4 ug/mg dry weight D:from 4 to 2 ug/mg dry tissue at 24 h, after 5 days 0.1 ug/mg dry weight | no | difference unclear | no | no | no | https://link.springer.com/article/10.1007/s10047-015-0819-z#Sec2 | ||
| 80 | vessel decellularization | Park et al | 2025 | fully biologic endothelialized tissue engineered vascular conduits provide antithrombotic function and graft patency | human umbilical arteries | CHAPS, ETA NaCl, SDS | 3 days | PBS | yes | yes | no | no | no | yes | no | no | yes | no | yes | yes | no | no | no | no | suture retention 6-0 prolene and 5mm, stress train, coherence tomography, | endothelial cells | no | no since focus on implantation | https://www.sciencedirect.com/science/article/pii/S1934590924004065?via%3Dihub#sec2 | |||||
| 81 | vessel decellularization | Sergeevichec et al. | 2023 | preservation of mechanical and morphological properties of porcine cardiac outflow vessels after decellularization and wet storage | porcine aorta | SDS SDC | static | 2 days | no | no | PBS | yes | no | no | no | no | no | no | no | no | no | no | no | yes | nuclear smudge present | no | no | uniaxial tensile testing | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC10807022/#sec3-biomimetics-08-00315 | |||
| 82 | vessel decellularization | cai et al. | 2019 | decellularization cross linking and heparin immobilization of porcine carotid arteries for tissue engineering vascular grafts | porcine carotid arteries | SDS Triton | static | 5 days | no | no | PBS | yes | yes | yes | no | no | no | no | no | yes | no | no | no | yes | no visible nuclei | clear difference | stress strain suture strength and burst pressure7-0 ethilon 2 mm | subcutaneous embedding | no | https://link.springer.com/article/10.1007/s10561-019-09792-5#Sec2 | ||||
| 83 | vessel decellularization | Gil-Ramirez et al. | 2020 | pressureized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes | porcine pulmonary arteries | CO2 | pressure | from 90-120 min | benzanoase | 90 U/ml | no | yes | YES | no | no | no | no | no | no | no | no | yes | no | no | nuclei present, but not in the DNAse treated samples | reduction of 93% | no | no | no | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC7055267/#Sec16 | ||
| 84 | vessel decellularization | Xia et al. | 2024 | the potential of a new natural vessel source decellularized intercoastal arteries as sufficiently long small diameter vascular grafts | bovine and porcine intercoastal arteries | Triton SDS | pressure | 2-4 days | no | no | PBS | yes | no | no | yes | no | no | no | yes | yes | no | yes | no | yes | porcine looks better than bovine . Porcine no nuclear content visible. Bovine some nuclear content leftover | N porcine: 400 ng/mg. D porcine: 24 h 200 ng/mg, 48 h 40 ng/mg. N bovine: 410 ng/mg. D bovine 24h 150 ng/mg tissue 48h 40 ng/mg. Unclear if wet or dry tissue | clear difference | tensile strength and burst pressure | HUVECS 96 well plate | yes | https://pmc.ncbi.nlm.nih.gov/articles/PMC11273892/#sec2-bioengineering-11-00700 | |||
| 85 | vessel decellularization | Chekhoeva et al. | 2021 | dichloroacetate inhibits the degeneration of decellularized cardiovascular implants | rat aorta | SDS SDC sodium azide | 2 days | Dnase | 2.3 mg/ml | PBS | yes | no | no | no | no | yes | no | no | no | yes | no | no | no | no | no | no | no | no | no | no | no since focus on recell | https://pmc.ncbi.nlm.nih.gov/articles/PMC8715846/#sec7 | ||
| 86 | vessel decellularization | Tardalkar et al | 2022 | heparin immbolization of tissue engineered xenogeneic small diameter arterial scaffold improve endothelialization | goat arteries | SDS or SDS+SDC, pr SDS+DMSO | static | several days | no | no | no | yes | no | yes | no | no | no | no | yes | yes | no | yes | no | yes | nuclear smudge present | no | difference unclear | biocompatibilia assay with implantation | no | no | https://pmc.ncbi.nlm.nih.gov/articles/PMC9130405/#Sec2 | |||
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| LIT WORKUP FOR THE USE OF DMSO IN DECELLULARIZATION | |||||||||||||
| Running number | Key words in search | Author(s) | Year | Title | Tissue type | Method | Results | Mechanism | Benefits | Disadvantages | Link to paper | ||
| stat. Perf. | decell time | concentration | |||||||||||
| 1 | Aorta SDS | Guler et al. | 2017 | improvement of decellularization efficiency of porcine aorta using dimethyl sulfoxide as a penetration enhancer | porcine aorta | static | 3 hr | 1 M in 3 L | better penetration for SDS. | Exact mechanism is not explained. | faster, more complete decellularization | possible cytotoxicity | papers\121006-improvement of decellularization efficiency of porcine aorta using dimethyl sulfoxide as a penetration enhancer.pdf |
| 2 | DMSO decellularization | Vasquez-Rivera et al. | 2017 | Simultaneous monitoring of different vitrification solution components permeating into tissues | porcine heart | static | 4d | 3.5 M DMSO | DMSO has a fast diffusion time within a decellularized tissue in comparison with sucrose or DMSO-sucrose mixture | tissue dehydration occured. The diffusion coefficient of DMSO is higher than sucrose | DMSO almost completely penetrates after 30 min, whereas sucrose predominantly resides in the outer regions of the tissue. | causes decrease in sample thickness, which might account for the apparent diffusivity | papers\121105-Simultaneous monitoring of different vitrification solution components permeating into tissues.pdf |
| 3 | DMSO decellularization | Hellström et al. | 2014 | Towards the development of bioengineered uterus: comparison of different protocols for rat uterus decellularizaiton. | rat uterus | perfusion | 5d | 4% (in PBS) | DMSO was not a very good decellularizing solution in comparison with sodium azide and SDC. | cell membrane disrupting agent | not very good preservation of scaffold | papers\121106-Towards the development of a bioengineered uterus comparison of different protocols for rat uterus decellularization.pdf | |
| 4 | DMSO decellularization | Alshaikh et al. | 2019 | Decelllularization of the mouse ovary: comparison of different scaffold generation protocols for future ovarian bioengineering | mice ovary | static | 3d | 4% DMSO with 1% triton X-100 | SDS and SDC were more effective in removing DNá rapidly from the ovaries in comparison with the Triton + DMSO combination | Exact mechanism is not explained. A combination of Triton x-100 and DMSO was used | the combination of Triton and DMSO was efficient for this group's rat uterus decellularization | not effective for breaking down DN'a | papers\121107-Decellularization of the mouse ovary comparison of different scaffold generation protocols for future ovarian bioengineering.pdf |
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| LIT WORKUP FOR THE USE OF SDS IN DECELLULARIZATION | ||||||||||||||
| Running number | Key words in search | Author(s) | Year | Title | Tissue type | Method | Results | Mechanism | Benefits | Disadvantages | Link to paper | |||
| detergent details | stat. Perf. | decell time | concentration | |||||||||||
| 1 | decell aorta | Fitriatul et al. | 2017 | Evaluation of Recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment | porcine aorta | SDS | perfusion combined with ultrasonic treatment | 10hr | 0.02 | their previous protocol had used only 0.1% SDS, they therefore increased the concentration leading to a better decellularization | the 2% SDS improves the conductivity of electric current in the washing of the detergent | higher concentration is better | in the HE histo it can be seen that the collagen fibers become too disrupted with a higher concentration of SDS | papers\088-Evaluation of recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment.pdf |
| 2 | decell aorta | Hazwani et al. | 2018 | Structural integrity of aortic scaffolds decellularized by sonication deellularization system | porcine aorta | SDS | ultrasonic treatment at a freq of 170 kHz | 10hr and washed for 5 days in PBS | 0.1% SDS | the detergent concentration was enough to remove cellular content according to HE | the combination of ultrasonic treatment and detergent was used for complete cell removal | cell removal is sufficient | No DN.a analysis was done. SEM images are not shown. No collagen tests performed. | papers\089-structural integrity of aortic scaffolds decellularized by sonication decellularization system.pdf |
| 3 | decell aorta | syazwani et al. | 2015 | decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering | porcine aorta | SDS | sonication treatment | 24hr | 2% SDS | there was a comparison of 2% SDS treatment with and without sonication. There was a 23% decrease in leftover DN#a with sonicaiton treatment. | the combination of sonication treatment and detergent was used to increase cell removal | Sonication increases DN'A removal | Structure is too disrupted: collagen fibers become distorted | papers\090-Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering.pdf |
| 4 | decell aorta | Reid and Callanan | 2019 | hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering | bovine aorta | SDS | perfusion | 36hr | 0.5% w/v at 170 mL/min | HE staining confirmed removal of cells. Picrosirius red staining confirmed a maintained collagen structure | only perfusion of low concentration of SDS | proper removal of cellular content | Decellularization takes too long | papers\093-hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering.pdf |
| 5 | decell aorta | Liu et al. | 2019 | an innovative method to obtain porous porcine aorta scaffolds for tissue engineering | porcine aorta | SDS | porfusion and vacuum freezing | not given | not given | they did a comparison of a high concentration SDS solution (exact conc. not given), and a vacuum freezing with low concentration SDS (exact conc. not given). The HE for high concentration shows disrupted collagen fibers and blue smears. The vacuum freezing with low concentration shows more retained collagen fibers and some blue smears | high concentration of SDS in perfusion compared with vacuum freezing with low concentration of SDS | maintainance of collagen fibers is better in the low concentration SDS combined with vaccuum freezing. | there is insufficient removal of cellular content (blue smears are visible). The blue smears are visible in both cases, but the collagen fibers are not well maintained with the higher concentration of SDS | papers\094-an innovative to obtain porous porcine aorta scaffolds for tissue engineering.pdf |
| 6 | decell aorta | fercana et al. | 2014 | platform technologies for decellularization, tunic specific cell seeding, and in vitro conditioning of extended length, small diameter vascular grafts. | bovine femoral arteries | SDS | perfusion: applying pressure on both sides so that the solvent diffuses into the wall of the arteries | 12 days | 1%SDS at 0.5L/min | There is a reduction of 95% DN'a content, HE shows well kept collagen structure and no visible nucleic stain. Mechanical tests do not seem to make sense | diffusion of SDS through the arterial wall for complete decellularization of the tissue | the collagen fibers are well mantained and the removal of cellular content seems complete. | complete process takes more than a week to complete | papers\121100-platform technologies for decellularization, tunic-specific cell seeding, and in vitro conditioning of extended length, small diameter vascular grafts.pdf |
| 7 | sdc decell | Fischer et al. | 2017 | comparative characterization of decellularized renal scaffolds for tissue engineering | porcine kidney | SDC/Triton/SDS | static | 10 days | 1% SDS | tissue seemed translucent after decellularization. | only the decell at 4 preserved tubules | matrix protiens (laminin, fibronectin, collagen IV) are preserved | tissue shrinkage and some incomplete decellularization | papers\121108- Comparative characterization of decellularized renal scaffolds for tissue engineering.pdf |
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| LIT WORKUP FOR THE USE OF SDC IN DECELLULARIZATION | ||||||||||||||
| Running number | Key words in search | Author(s) | Year | Title | Tissue type | Method | Results | Mechanism | Benefits | Disadvantages | Link to paper | |||
| detergent details | stat. Perf. | decell time | concentration | |||||||||||
| 1 | Decell rat aorta SDS | Fitzpatrick et al. | 2010 | effect of decellularization protocol on the mechanical behaviour of porcine descending aorta | porcine descending aorta | SDC+Triton | static | 24 hr | 0.25% SDC + 0.25% Triton | HE shows some nuclei (no images shown in article) | SDC "is regarded as a more disruptive detergent than SDS" | effective decellularization of the tissue | "more disruptive combination for the tissue" | papers\121026-effect of decellularization protocol on the mechanical behaviour of porcine descending aorta.pdf |
| 2 | Aorta | Kohrramirouz et al. | 2014 | Effect of three decellularization protocols on the mechanical behavious and strucural properties of sheep aortic valve conduits | sheep aortic valve | SDS+SDC/Triton+EDTA/Trypsin | static | 48h | 1% SDC + 1%SDS | effective for complete removal of cellular content. | no exact mechanism given | complete removal of cellular content | "relatively" good structural integrity. Mild decrease in elastin content compared to the control | papers\048-effect of three decellularization protocols on the mechanical behaviour and structural properties of sheep aortic valve conduits.pdf |
| 3 | sdc decell | Fischer et al. | 2017 | comparative characterization of decellularized renal scaffolds for tissue engineering | porcine kidney | SDC/Triton/SDS | static | 10 days | 1% SDC | tissue has more transparent appearance after decell, complete removal of cellular content | the most complete decell was done at 4 C | complete removal of cellular content | takes too long | papers\121108- Comparative characterization of decellularized renal scaffolds for tissue engineering.pdf |
| 4 | decellularization SDC | Roosens et al. | 2016 | impact of detergent based decellularization methods on procine tissues for heart valve engineering | porcine aortic valve | SDS/Triton/trypsin | static | 48 hr | 1% SDC and 2% SDC | incomplete decellularization | disruption of ECM | none mentioned | presence of leftover DN'a after decellularization was visualized through DAPI | papers\121109-Impact of detergent-based decellularization methods on porcine tissues for heart valve engineering.pdf |
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| LIT WORKUP FOR THE USE OF TRITON X-100 IN DECELLULARIZATION | ||||||||||||||
| Running number | Key words in search | Author(s) | Year | Title | Tissue type | Method | Results | Mechanism | Benefits | Disadvantages | Link to paper | |||
| detergent details | stat. Perf. | decell time | concentration | |||||||||||
| 1 | Decell rat liver | Hillebrandt et al. | 2015 | Procedure for decellularization of rat livers in an oscillating-pressure perfusion device | rat liver | 1% Triton X-100 and then 1% SDS | perfusion | 180 min | 1% Triton for 90 min and 1% SDS for 90 min | complete removal of hepatocytes | diffusion through the walls | complete removal of cellular content, as well as removal of DN'A content | decellularization was performed in rat liver | papers\068-procedure for decellularization of rat livers in an osicllating-pressure perfusion device.pdf |
| 2 | Decell rat aorta SDS | Grauss et al. | 2003 | decellularization of rat aortic valve allografts reduces leaflet destruction and extracellular matrix remodeling | aortic valve | SDS/ Triton/ Trypsin/ Triton+Trypsin | static | 24 hr/ 24hr/0.5hr/ 0.5to24hr | 1%-5% triton, 0.5% trypsin, 0.5%/1-5% trypsin+triton, 0.1%-1% SDS | there was a comparison of protocols using Triton, trypsin, triton+trypsin, SDS. For different amount of times | no exact mechanism given | combination of Triton with trypsin resulted in complete removal of cellular content | using only triton showed no change in cellularity compared with normal. | papers\121087-decellularization of rat aortic valve allografts reduces leaflet destruciton and extracellular matrix remodeling.pdf |
| 3 | Decell rat aorta SDS | Fitzpatrick et al. | 2010 | effect of decellularization protocol on the mechanical behaviour of porcine descending aorta | porcine descending aorta | Triton/ EDTA | static | 24 hr/15hr/24 hr | 1% triton and 0.02% EDTA | HE shows some nuclei (no images shown in article) | Triton disrupts lipid-lipd and lipid-protein interactions. EDTA removes cellular material by binding to divalent cations at cellular adhesion integris | no benefit | no complete decellularization | papers\121026-effect of decellularization protocol on the mechanical behaviour of porcine descending aorta.pdf |
| 4 | decell aorta | negishi et al. | 2017 | evaluation of small diameter vascular grafts reconstructed from decellularized aorta sheets | porcine aorta | SDS / triton | static | 15h or 3d | 0.5% Triton for 3 days | incomplete removal of cellular content as shown in HE | no exact mechanism given | no benefit from using the detergent on ist own | using the detergents alone leads to incomplete cell removal | papers\092-evaluation of small diameter vascular grafts reconstructed from decellularized aorta sheets.pdf |
| 5 | aorta decell | jiang et al. | 2015 | a polymer extracellular matrix compostie with improved thromboresistance and decellularization properties | rat aorta | Triton x-100 + SDS | perfusion | 4 days | 1% Triton for 48 hours followed by 1.5% SDS for 48 hours | complete removal of cellular content as shown by HE and DAPI, SEM showed surface topography similar to native | no exact mechanism given | complete removal of cellular content, as well as removal of DN'A content | decellularization took too long | papers\121101-A polymer-extracellular matrix composite with improved thromoresistance and recellularization properties.pdf |
| 6 | Aorta | Kohrramirouz et al. | 2014 | Effect of three decellularization protocols on the mechanical behavious and strucural properties of sheep aortic valve conduits | sheep aortic valve | SDS+SDC/Triton+EDTA/Trypsin | static | 1% triton+EDTA | observed cells and nuclei | no exact mechanism given | very good collagen preservation in all 3 layers | incomplete cell removal | papers\048-effect of three decellularization protocols on the mechanical behaviour and structural properties of sheep aortic valve conduits.pdf | |
| 7 | sdc decell | Fischer et al. | 2017 | comparative characterization of decellularized renal scaffolds for tissue engineering | porcine kidney | SDC/Triton/SDS | static | 10 days | 1% Triton | non transparent tissue after decellularizaiton under all used conditions. Cells were still visible in histology, and Dn'a was still visible in DAPI staining | no exact mechanism given | good preservation of ECM strucutre | incomplete cell removal | papers\121108- Comparative characterization of decellularized renal scaffolds for tissue engineering.pdf |
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| literature workup for: SEM images | |||||||||||||||
| NOTE: LIT WORKUP NOT YET FINISHED | |||||||||||||||
| number | Author(s) | Title | Tissue type | Decell Method | enzyme | Analysis SEM | Result | comment | Accepted? | link to paper | |||||
| Image | Notes | adventitia | fibers | ||||||||||||
| 1 | Guler et al. | improvement of decellularization efficiency of porcine aorta using dimethyl sulfoxide as a penetration enhancer | porcine aorta | SDS+DMSO/ SDS | static | Dnase | |||||||||
| 2 | Kohrramirouz et al. | Effect of three decellularization protocols on the mechanical behavious and strucural properties of sheep aortic valve conduits | sheep aortic valve | SDS+SDC/Triton+EDTA/Trypsin | ? | Rnase, Dnase | |||||||||
| 3 | Fitriatul et al. | Evaluation of Recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment | porcine aorta | SDS | ultrasonic treatment | no | |||||||||
| 4 | syazwani et al. | decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering | porcine aorta | SDS | ultrasonic treatment | no | |||||||||
| 5 | Ma et al. | development and in vivio validation of tissue-engineered samll diameter vascular grafts from decellularized aortae of fetal pigs and canine vascular endothelial cells | fetal porcine aorta | trypsin | static | Rnase, Dnase | |||||||||
| 6 | negishi et al. | evaluation of small diameter vascular grafts reconstructed from decellularized aorta sheets | porcine aora | SDS or triton | static | no | |||||||||
| 7 | Reid and Callanan | hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering | bovine aorta | SDS | static | no | |||||||||
| 8 | Weymann et al. | Total Aortic Arch Replacement: Superior Ventriculo-Arterial coupling with decellularized allografts compared with conventional prostheses | canine aortic arches | SDS and NaN3 | static | no | |||||||||
| 9 | jiang et al. | a polymer extracellular matrix compostie with improved thromboresistance and decellularization properties | rat aorta | Triton x-100 + SDS | perfusion | Dnase | |||||||||
| 10 | Pennel et al. | the performance of cross linked acellular arterial scaffolds as vascular grafts; preclinical testing in direct and isolation loop circulatory models | porcine renal arteries | 1M NaOH | not stated | no | |||||||||
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| LIT WORKUP FOR MECHANICAL TESTING | ||||||||||||||||||||
| Running number | Key words in search | Author(s) | Year | Title | Tissue type | sample information | Results | Mechanism | Benefits | Disadvantages | Link to paper | |||||||||
| test type | wall thickness um | intima media thickness um | length of sample | ultimate stress | ultimate strain | ultimate strength | ||||||||||||||
| 1 | sent by vladi | kubikova et al. | 2017 | the composition and biochemical properties of human cryopreserved aortas, pulmonary trunks, and aortic and pulmonary cusps | pulmonary and aortic heart valves | uniaxial | 2399 | 1864 | not given | 0.6 | 2.67 | |||||||||
| 2 | sent by vladi | Kochova et al. | 2012 | the contribution of vascular smooth muscle, elastin and collagen on the passive mechanics of porcine carotid arteries | porcine carotid arteries | inflation-deflation | 600 | 6-7 cm | ||||||||||||
| 3 | sent by vladi | kochova et al. | 2008 | morphology and mechanical properties of the subrenal aorta in nomotensive and hypertensive rats | subrenal aorta | 109 | 2 cm | 0.68 | 0.93 Mpa | |||||||||||
| 4 | sent by vladi | burkert et al. | 2020 | the time has come to extend the expiration limit of cryopreserved allograft heart valves | review paper | |||||||||||||||
| 5 | pressure arteriopgraph | Bowqridge et al. | 2019 | Maternal engineered nanomaterial inhalation during gestation disrupts vascular kisspeptin reactivity | uterine artery | impact of E2 and kiss in vascular dyfunction | 54 | 50 um | not given | use of pressure arteriograh | no mechanical testing | ..\08.Full_papers\121126-Maternal Engineered Nanomaterial Inhalation During Gestation Disrupts Vascular Kisspepting Reactivity.pdf | ||||||||
| 6 | pressure arteriopgraph | Johnson et al. | 2019 | memory impairment in spontaneously hypertensive rats is associated with hippocampal hypoperfusion and hippocampal vascular dysfunction | hippocampal vasculature | oxygenation of hippocampus | 5mm | not done | not done | not done | max pressure 200 mmHg | use of pressure arteriograh | no mechanical testing | ..\08.Full_papers\121127-Memory impairment in spontaneously hypertensive rats is associated with hippocampal hypoperfusion and hippocampal vascular dysfunction.pdf | ||||||
| 7 | pressure arteriopgraph | McCarthy et al. | 2019 | reconstitution of autophagy ameliorates vascular function and arterial stiffening in spontaneously hypertensive rats | mesenteric resistance arteries | 40 | not given | 1.2 x10°6 dynes. Cm°-2 | 2.0 | not done | measurement of wall expansion depending on pressure within the vessel as a test of vascular mechanics | use of pressure arteriograh | only pressure applied as force | ..\08.Full_papers\121128-Reconstitution of autophagy ameloriates vascular functin and arterial stiffening in spontaneously hypertensive rats.pdf | ||||||
| 8 | pressure myograph | Miller et al. | 2019 | impact pf diet on the persistance of early vascular remodeling and stiffening induced by intrauterine growth restriction and maternal high fat diet | carotid artery | arterial stiffness through stretch | not done | not done | not done | not done | not done | not done | systolic and diastolic blood pressure measurements, compared with the transmural pressure against stretch of the vessel | use of pressure myograph | no mechanical testing | ..\08.Full_papers\121129-Impact of diet on the persistance of early vascular remodeling and stiffeninf induced by intrauterine growth restriction and maternal high fat diet.pdf | ||||
| 9 | mechanical vessel | Ziegler and Nerem | 1994 | Tissue Engineering a Blood Vessel: Regulation of Vascular biology by mechincal stresses | cell culture of SMC and EC | none | not done | not done | not done | not done | not done | not done | EC prperties, SMC contractile phenotype, SMC density, ECM compostition, Tensile strength, shear stress, stretching and pressure | none | none | ..\08.Full_papers\121130-Tissue Engineering a Blood Vessel regulation of vascular biology by mechanical stresses.pdf | ||||
| 10 | strain vessel | Seliktar et al. | 2003 | Mechanical strain-stimulated remodeling of tissue engineered blood vessel constructs | human aortic smooth muscle cells and rat aortic smooth muscle cells | hoop stress: ccauses radial and cirumferential strains on SMCs. | not done | not done | 5 mm | RSMC: normal ultimate stress: 10kPa. after 4 days 17kPa,,, HSMC native ultimate stress is 4.5 kPa, after 4 days it is 22 kPa. | not done | not done | a ring was placed between two hoops and strained for different amounts of time at 0.2 mm/s. The ultimate stress was then calculated for each tissue type and time of stress. | none | none | ..\08.Full_papers\121131-Mechanical strain-stimulated remodeling of tissue engineered blood vessel constructs.pdf | normal blood pressure: 10.8 kPa. Prehypertension: 13.2 kPa, stage 1 hypertension: 15.0 kPa, stage 2 hypertension: 16.7 kPa, stage 3 hypertension: 17.7 kPa. | |||
| 11 | mechanical strain vessel | Yang et al. | 2019 | Comparative study of variations in mechanical stress and strain of human blood vessels: mechanical reference for vascular cell mechano-biology | biaxial extension | not given | not given | not given | 311.6 kPa/mm | normal: 10.8 kPa. For thor aorta strain: 0.25 kPa. | not done | axial stretch (method not clarified) | conclusion that specific locations and phyiological functions determine the mechanical properties of human arteries. | ..\08.Full_papers\121132-Comparative study of variations in mechanical stress and strain of human blood vessels mechanical reference for vascular cell mechano-biology.pdf | ||||||
| 12 | mechanical strain vessel | Huang et al. | 2009 | Mechanical strain induces expression of C-reactive protein in human blood vessels | saphenous veins | none | not given | not given | 3 mm | not done | not done | not done | used a force transducer similar to what is sold by scintica. | only strained the vessels in order to induce the expression of different proteins by SMCs. | ..\08.Full_papers\121133-Mechanical strain induces expression of C-reactive protien in Human blood vessels.pdf | |||||
| 13 | mechanical testing vessels | Wang et al. | 2020 | artificial small diameter blood vessels: materials, fabrication, surface modification, mehcanical properties and bioactive functionalities | review paper regarding the generation of small diameter vessel grafts | youngs modulus: strain/stress: tissue stiffness. Nonlinear elasticity: radial expansion which is a low circumferential youngs modulus (20-50 kPa). Compliance measurements: is the chnage of the tube that occirs in response to pressure variations within the lumen. this could lead to damage in the endothelial layer. Burst pressure measurements: is the maximum pressure that a vascular vessel can withstand before an accute leak occurs and it fails. suture retention test: measures the adhesion of an implant to surrounding tissue. it is a kew issue for surgery as its resistance to the stresstes associated with implantation can only be established by determining the force necessary to pull a suture through a wall. | ..\08.Full_papers\121134-Artificial Small diameter Blood vessels- Materials, Fabrications, Surface modification, mechanical properties, bioactive functionalities.pdf | |||||||||||||
| 14 | mechanical testing vessels | solan et al. | 2009 | effects of mechanical stretch on collagen and cross linking in engineered blood vessels | cell culture of VSMC on a polymeric tube | ..\08.Full_papers\121135-effects of mechanical stretch on collagen and cross linking in engineered blood vessels.pdf | ||||||||||||||
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| number | Key words in search | Author(s) | Year | Title | Tissue type | clinical info | comment | Accepted? | link to paper | ||
| age | weight | flow rate | |||||||||
| 1 | flow rate piglet carotid | Olver et al. | 2016 | carotid artery vascular mechanics serve as biomarkers of cognitive dysfunction in aortic-banded miniature swine that can be treated with an exercise intervention | yucatan pig's carotid artery | 8 months | n/a | 400 ml/min | yes | ..\08.Full_papers\121117-Carotid artery vascular mechanics serve as biomarkers of cognitive dysfunction in aortic-banded minature swine that can be treated with an excercise intervention.pdf | |
| 2 | pig carotid artery | marcel et al. | 1995 | carotid blood flow distribution, haemodynamics and inotropic responses following calcitonin gene-realted peptide in the pig | yorkshire pig | n/a | 15-17 kg | 50 ml/min | blood flow was increased from baseline | no | ..\08.Full_papers\121118-carotid blood flow distribution, haemodynamics and inotropic responses following calcitonin gene related peptide in the pig.pdf |
| 3 | pig carotid artery | Ishii et al. | 2005 | Swine Model of carotid artery atherosclerosis: experimental induction by surgical partial ligation and dietary hypercholesterolemia | yucatan mini pigs | 6 months | 20-30 kg | n/a | good explanation of funciton of endothelial layer | no | ..\08.Full_papers\121119-swine model of carotid artery atherosclerosis experimental induction by surgical partial ligation and dietary hypercholesterolemia.pdf |
| 4 | pig carotid artery | Mangla et al. | 2015 | Endovascular external carotid artery occlusion for brain selective targeting: a cerebrovascular swine model | yorkshire swine | 18-36 weeks | 35-55 kg | n/a | flow rate in brain (not in carotid!) | no | ..\08.Full_papers\121120-endovascular external carotid artery occlusion for brain selective targeting a cerebrovascular swine model.pdf |
| 5 | pig carotid artery | Saez et al. | 2016 | Microstructural quantification of collagen fiber orientations and ist integration in constitutive modeling of porcine carotid artery | porcine common carotid arteries | n/a | n/a | n/a | paper is useful for mechanical testing ! | no | ..\08.Full_papers\121121-Microstructural quantificatin of collagen fiber orientations and its integration in constitutive modeling of the porcine carotid artery.pdf |
| 6 | pig carotid artery blood flow | Mrowczynski et al. | 2014 | porcine carotid artery replacement with biodegradable electrospun poly-e-caprolactone vascular prosthesis | land race pigs | n/a | 30-45 kg | 260 ml/min | yes | ..\08.Full_papers\121122-Porcine carotid artery replacement with biodegradable electrospun poly-e-caprolactone vascular prosthesis.pdf | |
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| Running number | Key words in search | Author(s) | Year | Title | Tissue type | Method | DNAse concentration | DNAse time | MgCl2 | Results | Mechanism | Benefits | Disadvantages | Link to paper | ||
| stat. Perf. | decell time | concentration | ||||||||||||||
| 1 | dnase decellularization | McCrary et al. | 2020 | novel sodium deoxycholate based chemical decellularization method for peripheral nerve | nerves | perf | over 3 days | 3% SDC | 75U/mL | 3 hr | ..\08.Full_papers\121136-novel sodium deoxycholate based chemical decellularization method for peripheral nerve.pdf | |||||
| 2 | dnase decellularization | Ahearne,M. | 2020 | corneal extracellular matrix decellularization | pig corneas | perf | over 5 dyas | 0.5% SDS+5%tritonx-100 | 10U/mL +(10U/mL Rnase) | 1 hr at 37C | ..\08.Full_papers\121137-corneal extracellular matrix decellularization.pdf | |||||
| 3 | dnase decellularization | Yamanaka et al. | 2020 | decellularization of submillimeter-diameter vascular scaffolds using peracetic acid | rat tail arteries | perf | over 3 days | 0.1% PAA +4%EtOH | 40U/mL | 3 days at 37C | 20 mM | ..\08.Full_papers\121138-decellularization of submillimeter-diameter vascular scaffolds using peracetic acid.pdf | ||||
