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DNA content aorta
Aorta decell.
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SEM images
mechanical testing
flow rate pig carotid
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| Literature work up for: DNA CONTENT RAT AORTA | NOTE: REDO THIS LIST! IT IS NOT VERY WELL MADE | ||||||||||
| number | Key words in search | Author(s) | Year | Title | Vessel type | Method | Analysis | Result | comment | Accepted? | |
| drying method (if avialble) | |||||||||||
| 1 | rat aorta DNA content | Capron et al. | Growth-promoting effect of diabetes and insulin on arteries an in vivo study of rat aorta | 75.7 ug | no further units for DNAresult | no | |||||
| 2 | rat aorta DNA content | Olivetti et al. | Quantitiative Structural changes of the rat thoracic aorta in early spontaneous Hyertension | 240 ug | |||||||
| 3 | rat aorta DNA content | Evensen & Shepro | 1974 | DNA synthesis in rat aortic endothelium: effect of bacterial Endotoxin and trauma | Rat Aorta | no | rapid autoradiography | H staining | 10 cells/10 mm^2 | no DNAisolation | no |
| 4 | rat aorta DNA content | Berry et al. | 1972 | The growth and development of the rat aorta: changes in nucleic acid and scleroprotein content | liquid nitrogen | shibko et al. | used wet weight only | ||||
| 5 | vessel decellularization and DANN | Li et al. | 2013 | The Fetal Porcine Aorta and Mesenteric Acellular Matrix as Small-caliber Tissue Engineering Vessels and Microvasculature Scaffold | fetal porcine aorta | Proteinase K 37° for 144 hr | spectrophotometry (280 nm) | 271.10 ng/ul | no tissue weight reference | jaein | |
| 6 | vessel decellularization and DANN | Mancuso et al. | 2015 | Decellularized ovine arteries as small-diameter vascular grafts | ovine arteries | PureLink Genomic DNAKit, Invitrogen | PureLink Genomic DNAKit, Invitrogen | spectrophotometry (260 nm) | 150 ng/mg dry weight | used 25 mg dried tissue | jaein |
| 7 | vessel decellularization and DANN | Guler et al. | 2017 | improvement of decellularization efficiency of porcine aorta using dimethyl sulfoxide as penetration enhancer | porcine aorta | freeze dried | freeze dried, Protienase K, Quant-iT | pico green fluorescence (520 nm) | 6900 ng/10 mg dry tissue | about 690 ng/mg tissue | jaein |
| 8 | Liver decell | Coronado et al. | 2017 | Decellularization and Solubilization of Porcine Liver dor Use as Substrate for Porcine Hepatocyte Culture: Method Optimization and Comparison | Porcine LIVER | lypophilization | Quant-iT and Dneasy Blood & tissue kit | pico green fluorescence (520 nm) | 3000 ng/mg dry tissue | PORCINE LIVER | yes |
| 9 | Liver decell | Wang et al. | 2014 | Method for perfusion decellularization of porcine whole liver and kidney for use as scaffold for clinical-scale bioengineering engrafts | Poricne Liver | N/A | Tissue DNAeasy Kit (tiangen Biotech) | N/A | 1000 ng/mg dry weight | PORCINE LIVER | no |
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| LIT WORKUP FOR THE USE OF DMSO IN DECELLULARIZATION | |||||||||||||
| Running number | Key words in search | Author(s) | Year | Title | Tissue type | Method | Results | Mechanism | Benefits | Disadvantages | Link to paper | ||
| stat. Perf. | decell time | concentration | |||||||||||
| 1 | Aorta SDS | Guler et al. | 2017 | improvement of decellularization efficiency of porcine aorta using dimethyl sulfoxide as a penetration enhancer | porcine aorta | static | 3 hr | 1 M in 3 L | better penetration for SDS. | Exact mechanism is not explained. | faster, more complete decellularization | possible cytotoxicity | papers\121006-improvement of decellularization efficiency of porcine aorta using dimethyl sulfoxide as a penetration enhancer.pdf |
| 2 | DMSO decellularization | Vasquez-Rivera et al. | 2017 | Simultaneous monitoring of different vitrification solution components permeating into tissues | porcine heart | static | 4d | 3.5 M DMSO | DMSO has a fast diffusion time within a decellularized tissue in comparison with sucrose or DMSO-sucrose mixture | tissue dehydration occured. The diffusion coefficient of DMSO is higher than sucrose | DMSO almost completely penetrates after 30 min, whereas sucrose predominantly resides in the outer regions of the tissue. | causes decrease in sample thickness, which might account for the apparent diffusivity | papers\121105-Simultaneous monitoring of different vitrification solution components permeating into tissues.pdf |
| 3 | DMSO decellularization | Hellström et al. | 2014 | Towards the development of bioengineered uterus: comparison of different protocols for rat uterus decellularizaiton. | rat uterus | perfusion | 5d | 4% (in PBS) | DMSO was not a very good decellularizing solution in comparison with sodium azide and SDC. | cell membrane disrupting agent | not very good preservation of scaffold | papers\121106-Towards the development of a bioengineered uterus comparison of different protocols for rat uterus decellularization.pdf | |
| 4 | DMSO decellularization | Alshaikh et al. | 2019 | Decelllularization of the mouse ovary: comparison of different scaffold generation protocols for future ovarian bioengineering | mice ovary | static | 3d | 4% DMSO with 1% triton X-100 | SDS and SDC were more effective in removing DNá rapidly from the ovaries in comparison with the Triton + DMSO combination | Exact mechanism is not explained. A combination of Triton x-100 and DMSO was used | the combination of Triton and DMSO was efficient for this group's rat uterus decellularization | not effective for breaking down DN'a | papers\121107-Decellularization of the mouse ovary comparison of different scaffold generation protocols for future ovarian bioengineering.pdf |
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| LIT WORKUP FOR THE USE OF SDS IN DECELLULARIZATION | ||||||||||||||
| Running number | Key words in search | Author(s) | Year | Title | Tissue type | Method | Results | Mechanism | Benefits | Disadvantages | Link to paper | |||
| detergent details | stat. Perf. | decell time | concentration | |||||||||||
| 1 | decell aorta | Fitriatul et al. | 2017 | Evaluation of Recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment | porcine aorta | SDS | perfusion combined with ultrasonic treatment | 10hr | 0.02 | their previous protocol had used only 0.1% SDS, they therefore increased the concentration leading to a better decellularization | the 2% SDS improves the conductivity of electric current in the washing of the detergent | higher concentration is better | in the HE histo it can be seen that the collagen fibers become too disrupted with a higher concentration of SDS | papers\088-Evaluation of recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment.pdf |
| 2 | decell aorta | Hazwani et al. | 2018 | Structural integrity of aortic scaffolds decellularized by sonication deellularization system | porcine aorta | SDS | ultrasonic treatment at a freq of 170 kHz | 10hr and washed for 5 days in PBS | 0.1% SDS | the detergent concentration was enough to remove cellular content according to HE | the combination of ultrasonic treatment and detergent was used for complete cell removal | cell removal is sufficient | No DN.a analysis was done. SEM images are not shown. No collagen tests performed. | papers\089-structural integrity of aortic scaffolds decellularized by sonication decellularization system.pdf |
| 3 | decell aorta | syazwani et al. | 2015 | decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering | porcine aorta | SDS | sonication treatment | 24hr | 2% SDS | there was a comparison of 2% SDS treatment with and without sonication. There was a 23% decrease in leftover DN#a with sonicaiton treatment. | the combination of sonication treatment and detergent was used to increase cell removal | Sonication increases DN'A removal | Structure is too disrupted: collagen fibers become distorted | papers\090-Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering.pdf |
| 4 | decell aorta | Reid and Callanan | 2019 | hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering | bovine aorta | SDS | perfusion | 36hr | 0.5% w/v at 170 mL/min | HE staining confirmed removal of cells. Picrosirius red staining confirmed a maintained collagen structure | only perfusion of low concentration of SDS | proper removal of cellular content | Decellularization takes too long | papers\093-hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering.pdf |
| 5 | decell aorta | Liu et al. | 2019 | an innovative method to obtain porous porcine aorta scaffolds for tissue engineering | porcine aorta | SDS | porfusion and vacuum freezing | not given | not given | they did a comparison of a high concentration SDS solution (exact conc. not given), and a vacuum freezing with low concentration SDS (exact conc. not given). The HE for high concentration shows disrupted collagen fibers and blue smears. The vacuum freezing with low concentration shows more retained collagen fibers and some blue smears | high concentration of SDS in perfusion compared with vacuum freezing with low concentration of SDS | maintainance of collagen fibers is better in the low concentration SDS combined with vaccuum freezing. | there is insufficient removal of cellular content (blue smears are visible). The blue smears are visible in both cases, but the collagen fibers are not well maintained with the higher concentration of SDS | papers\094-an innovative to obtain porous porcine aorta scaffolds for tissue engineering.pdf |
| 6 | decell aorta | fercana et al. | 2014 | platform technologies for decellularization, tunic specific cell seeding, and in vitro conditioning of extended length, small diameter vascular grafts. | bovine femoral arteries | SDS | perfusion: applying pressure on both sides so that the solvent diffuses into the wall of the arteries | 12 days | 1%SDS at 0.5L/min | There is a reduction of 95% DN'a content, HE shows well kept collagen structure and no visible nucleic stain. Mechanical tests do not seem to make sense | diffusion of SDS through the arterial wall for complete decellularization of the tissue | the collagen fibers are well mantained and the removal of cellular content seems complete. | complete process takes more than a week to complete | papers\121100-platform technologies for decellularization, tunic-specific cell seeding, and in vitro conditioning of extended length, small diameter vascular grafts.pdf |
| 7 | sdc decell | Fischer et al. | 2017 | comparative characterization of decellularized renal scaffolds for tissue engineering | porcine kidney | SDC/Triton/SDS | static | 10 days | 1% SDS | tissue seemed translucent after decellularization. | only the decell at 4 preserved tubules | matrix protiens (laminin, fibronectin, collagen IV) are preserved | tissue shrinkage and some incomplete decellularization | papers\121108- Comparative characterization of decellularized renal scaffolds for tissue engineering.pdf |
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| LIT WORKUP FOR THE USE OF SDC IN DECELLULARIZATION | ||||||||||||||
| Running number | Key words in search | Author(s) | Year | Title | Tissue type | Method | Results | Mechanism | Benefits | Disadvantages | Link to paper | |||
| detergent details | stat. Perf. | decell time | concentration | |||||||||||
| 1 | Decell rat aorta SDS | Fitzpatrick et al. | 2010 | effect of decellularization protocol on the mechanical behaviour of porcine descending aorta | porcine descending aorta | SDC+Triton | static | 24 hr | 0.25% SDC + 0.25% Triton | HE shows some nuclei (no images shown in article) | SDC "is regarded as a more disruptive detergent than SDS" | effective decellularization of the tissue | "more disruptive combination for the tissue" | papers\121026-effect of decellularization protocol on the mechanical behaviour of porcine descending aorta.pdf |
| 2 | Aorta | Kohrramirouz et al. | 2014 | Effect of three decellularization protocols on the mechanical behavious and strucural properties of sheep aortic valve conduits | sheep aortic valve | SDS+SDC/Triton+EDTA/Trypsin | static | 48h | 1% SDC + 1%SDS | effective for complete removal of cellular content. | no exact mechanism given | complete removal of cellular content | "relatively" good structural integrity. Mild decrease in elastin content compared to the control | papers\048-effect of three decellularization protocols on the mechanical behaviour and structural properties of sheep aortic valve conduits.pdf |
| 3 | sdc decell | Fischer et al. | 2017 | comparative characterization of decellularized renal scaffolds for tissue engineering | porcine kidney | SDC/Triton/SDS | static | 10 days | 1% SDC | tissue has more transparent appearance after decell, complete removal of cellular content | the most complete decell was done at 4 C | complete removal of cellular content | takes too long | papers\121108- Comparative characterization of decellularized renal scaffolds for tissue engineering.pdf |
| 4 | decellularization SDC | Roosens et al. | 2016 | impact of detergent based decellularization methods on procine tissues for heart valve engineering | porcine aortic valve | SDS/Triton/trypsin | static | 48 hr | 1% SDC and 2% SDC | incomplete decellularization | disruption of ECM | none mentioned | presence of leftover DN'a after decellularization was visualized through DAPI | papers\121109-Impact of detergent-based decellularization methods on porcine tissues for heart valve engineering.pdf |
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| LIT WORKUP FOR THE USE OF TRITON X-100 IN DECELLULARIZATION | ||||||||||||||
| Running number | Key words in search | Author(s) | Year | Title | Tissue type | Method | Results | Mechanism | Benefits | Disadvantages | Link to paper | |||
| detergent details | stat. Perf. | decell time | concentration | |||||||||||
| 1 | Decell rat liver | Hillebrandt et al. | 2015 | Procedure for decellularization of rat livers in an oscillating-pressure perfusion device | rat liver | 1% Triton X-100 and then 1% SDS | perfusion | 180 min | 1% Triton for 90 min and 1% SDS for 90 min | complete removal of hepatocytes | diffusion through the walls | complete removal of cellular content, as well as removal of DN'A content | decellularization was performed in rat liver | papers\068-procedure for decellularization of rat livers in an osicllating-pressure perfusion device.pdf |
| 2 | Decell rat aorta SDS | Grauss et al. | 2003 | decellularization of rat aortic valve allografts reduces leaflet destruction and extracellular matrix remodeling | aortic valve | SDS/ Triton/ Trypsin/ Triton+Trypsin | static | 24 hr/ 24hr/0.5hr/ 0.5to24hr | 1%-5% triton, 0.5% trypsin, 0.5%/1-5% trypsin+triton, 0.1%-1% SDS | there was a comparison of protocols using Triton, trypsin, triton+trypsin, SDS. For different amount of times | no exact mechanism given | combination of Triton with trypsin resulted in complete removal of cellular content | using only triton showed no change in cellularity compared with normal. | papers\121087-decellularization of rat aortic valve allografts reduces leaflet destruciton and extracellular matrix remodeling.pdf |
| 3 | Decell rat aorta SDS | Fitzpatrick et al. | 2010 | effect of decellularization protocol on the mechanical behaviour of porcine descending aorta | porcine descending aorta | Triton/ EDTA | static | 24 hr/15hr/24 hr | 1% triton and 0.02% EDTA | HE shows some nuclei (no images shown in article) | Triton disrupts lipid-lipd and lipid-protein interactions. EDTA removes cellular material by binding to divalent cations at cellular adhesion integris | no benefit | no complete decellularization | papers\121026-effect of decellularization protocol on the mechanical behaviour of porcine descending aorta.pdf |
| 4 | decell aorta | negishi et al. | 2017 | evaluation of small diameter vascular grafts reconstructed from decellularized aorta sheets | porcine aorta | SDS / triton | static | 15h or 3d | 0.5% Triton for 3 days | incomplete removal of cellular content as shown in HE | no exact mechanism given | no benefit from using the detergent on ist own | using the detergents alone leads to incomplete cell removal | papers\092-evaluation of small diameter vascular grafts reconstructed from decellularized aorta sheets.pdf |
| 5 | aorta decell | jiang et al. | 2015 | a polymer extracellular matrix compostie with improved thromboresistance and decellularization properties | rat aorta | Triton x-100 + SDS | perfusion | 4 days | 1% Triton for 48 hours followed by 1.5% SDS for 48 hours | complete removal of cellular content as shown by HE and DAPI, SEM showed surface topography similar to native | no exact mechanism given | complete removal of cellular content, as well as removal of DN'A content | decellularization took too long | papers\121101-A polymer-extracellular matrix composite with improved thromoresistance and recellularization properties.pdf |
| 6 | Aorta | Kohrramirouz et al. | 2014 | Effect of three decellularization protocols on the mechanical behavious and strucural properties of sheep aortic valve conduits | sheep aortic valve | SDS+SDC/Triton+EDTA/Trypsin | static | 1% triton+EDTA | observed cells and nuclei | no exact mechanism given | very good collagen preservation in all 3 layers | incomplete cell removal | papers\048-effect of three decellularization protocols on the mechanical behaviour and structural properties of sheep aortic valve conduits.pdf | |
| 7 | sdc decell | Fischer et al. | 2017 | comparative characterization of decellularized renal scaffolds for tissue engineering | porcine kidney | SDC/Triton/SDS | static | 10 days | 1% Triton | non transparent tissue after decellularizaiton under all used conditions. Cells were still visible in histology, and Dn'a was still visible in DAPI staining | no exact mechanism given | good preservation of ECM strucutre | incomplete cell removal | papers\121108- Comparative characterization of decellularized renal scaffolds for tissue engineering.pdf |
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| literature workup for: SEM images | |||||||||||||||
| NOTE: LIT WORKUP NOT YET FINISHED | |||||||||||||||
| number | Author(s) | Title | Tissue type | Decell Method | enzyme | Analysis SEM | Result | comment | Accepted? | link to paper | |||||
| Image | Notes | adventitia | fibers | ||||||||||||
| 1 | Guler et al. | improvement of decellularization efficiency of porcine aorta using dimethyl sulfoxide as a penetration enhancer | porcine aorta | SDS+DMSO/ SDS | static | Dnase | |||||||||
| 2 | Kohrramirouz et al. | Effect of three decellularization protocols on the mechanical behavious and strucural properties of sheep aortic valve conduits | sheep aortic valve | SDS+SDC/Triton+EDTA/Trypsin | ? | Rnase, Dnase | |||||||||
| 3 | Fitriatul et al. | Evaluation of Recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment | porcine aorta | SDS | ultrasonic treatment | no | |||||||||
| 4 | syazwani et al. | decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering | porcine aorta | SDS | ultrasonic treatment | no | |||||||||
| 5 | Ma et al. | development and in vivio validation of tissue-engineered samll diameter vascular grafts from decellularized aortae of fetal pigs and canine vascular endothelial cells | fetal porcine aorta | trypsin | static | Rnase, Dnase | |||||||||
| 6 | negishi et al. | evaluation of small diameter vascular grafts reconstructed from decellularized aorta sheets | porcine aora | SDS or triton | static | no | |||||||||
| 7 | Reid and Callanan | hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering | bovine aorta | SDS | static | no | |||||||||
| 8 | Weymann et al. | Total Aortic Arch Replacement: Superior Ventriculo-Arterial coupling with decellularized allografts compared with conventional prostheses | canine aortic arches | SDS and NaN3 | static | no | |||||||||
| 9 | jiang et al. | a polymer extracellular matrix compostie with improved thromboresistance and decellularization properties | rat aorta | Triton x-100 + SDS | perfusion | Dnase | |||||||||
| 10 | Pennel et al. | the performance of cross linked acellular arterial scaffolds as vascular grafts; preclinical testing in direct and isolation loop circulatory models | porcine renal arteries | 1M NaOH | not stated | no | |||||||||
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| LIT WORKUP FOR MECHANICAL TESTING | ||||||||||||||||||||
| Running number | Key words in search | Author(s) | Year | Title | Tissue type | sample information | Results | Mechanism | Benefits | Disadvantages | Link to paper | |||||||||
| test type | wall thickness um | intima media thickness um | length of sample | ultimate stress | ultimate strain | ultimate strength | ||||||||||||||
| 1 | sent by vladi | kubikova et al. | 2017 | the composition and biochemical properties of human cryopreserved aortas, pulmonary trunks, and aortic and pulmonary cusps | pulmonary and aortic heart valves | uniaxial | 2399 | 1864 | not given | 0.6 | 2.67 | |||||||||
| 2 | sent by vladi | Kochova et al. | 2012 | the contribution of vascular smooth muscle, elastin and collagen on the passive mechanics of porcine carotid arteries | porcine carotid arteries | inflation-deflation | 600 | 6-7 cm | ||||||||||||
| 3 | sent by vladi | kochova et al. | 2008 | morphology and mechanical properties of the subrenal aorta in nomotensive and hypertensive rats | subrenal aorta | 109 | 2 cm | 0.68 | 0.93 Mpa | |||||||||||
| 4 | sent by vladi | burkert et al. | 2020 | the time has come to extend the expiration limit of cryopreserved allograft heart valves | review paper | |||||||||||||||
| 5 | pressure arteriopgraph | Bowqridge et al. | 2019 | Maternal engineered nanomaterial inhalation during gestation disrupts vascular kisspeptin reactivity | uterine artery | impact of E2 and kiss in vascular dyfunction | 54 | 50 um | not given | use of pressure arteriograh | no mechanical testing | ..\08.Full_papers\121126-Maternal Engineered Nanomaterial Inhalation During Gestation Disrupts Vascular Kisspepting Reactivity.pdf | ||||||||
| 6 | pressure arteriopgraph | Johnson et al. | 2019 | memory impairment in spontaneously hypertensive rats is associated with hippocampal hypoperfusion and hippocampal vascular dysfunction | hippocampal vasculature | oxygenation of hippocampus | 5mm | not done | not done | not done | max pressure 200 mmHg | use of pressure arteriograh | no mechanical testing | ..\08.Full_papers\121127-Memory impairment in spontaneously hypertensive rats is associated with hippocampal hypoperfusion and hippocampal vascular dysfunction.pdf | ||||||
| 7 | pressure arteriopgraph | McCarthy et al. | 2019 | reconstitution of autophagy ameliorates vascular function and arterial stiffening in spontaneously hypertensive rats | mesenteric resistance arteries | 40 | not given | 1.2 x10°6 dynes. Cm°-2 | 2.0 | not done | measurement of wall expansion depending on pressure within the vessel as a test of vascular mechanics | use of pressure arteriograh | only pressure applied as force | ..\08.Full_papers\121128-Reconstitution of autophagy ameloriates vascular functin and arterial stiffening in spontaneously hypertensive rats.pdf | ||||||
| 8 | pressure myograph | Miller et al. | 2019 | impact pf diet on the persistance of early vascular remodeling and stiffening induced by intrauterine growth restriction and maternal high fat diet | carotid artery | arterial stiffness through stretch | not done | not done | not done | not done | not done | not done | systolic and diastolic blood pressure measurements, compared with the transmural pressure against stretch of the vessel | use of pressure myograph | no mechanical testing | ..\08.Full_papers\121129-Impact of diet on the persistance of early vascular remodeling and stiffeninf induced by intrauterine growth restriction and maternal high fat diet.pdf | ||||
| 9 | mechanical vessel | Ziegler and Nerem | 1994 | Tissue Engineering a Blood Vessel: Regulation of Vascular biology by mechincal stresses | cell culture of SMC and EC | none | not done | not done | not done | not done | not done | not done | EC prperties, SMC contractile phenotype, SMC density, ECM compostition, Tensile strength, shear stress, stretching and pressure | none | none | ..\08.Full_papers\121130-Tissue Engineering a Blood Vessel regulation of vascular biology by mechanical stresses.pdf | ||||
| 10 | strain vessel | Seliktar et al. | 2003 | Mechanical strain-stimulated remodeling of tissue engineered blood vessel constructs | human aortic smooth muscle cells and rat aortic smooth muscle cells | hoop stress: ccauses radial and cirumferential strains on SMCs. | not done | not done | 5 mm | RSMC: normal ultimate stress: 10kPa. after 4 days 17kPa,,, HSMC native ultimate stress is 4.5 kPa, after 4 days it is 22 kPa. | not done | not done | a ring was placed between two hoops and strained for different amounts of time at 0.2 mm/s. The ultimate stress was then calculated for each tissue type and time of stress. | none | none | ..\08.Full_papers\121131-Mechanical strain-stimulated remodeling of tissue engineered blood vessel constructs.pdf | normal blood pressure: 10.8 kPa. Prehypertension: 13.2 kPa, stage 1 hypertension: 15.0 kPa, stage 2 hypertension: 16.7 kPa, stage 3 hypertension: 17.7 kPa. | |||
| 11 | mechanical strain vessel | Yang et al. | 2019 | Comparative study of variations in mechanical stress and strain of human blood vessels: mechanical reference for vascular cell mechano-biology | biaxial extension | not given | not given | not given | 311.6 kPa/mm | normal: 10.8 kPa. For thor aorta strain: 0.25 kPa. | not done | axial stretch (method not clarified) | conclusion that specific locations and phyiological functions determine the mechanical properties of human arteries. | ..\08.Full_papers\121132-Comparative study of variations in mechanical stress and strain of human blood vessels mechanical reference for vascular cell mechano-biology.pdf | ||||||
| 12 | mechanical strain vessel | Huang et al. | 2009 | Mechanical strain induces expression of C-reactive protein in human blood vessels | saphenous veins | none | not given | not given | 3 mm | not done | not done | not done | used a force transducer similar to what is sold by scintica. | only strained the vessels in order to induce the expression of different proteins by SMCs. | ..\08.Full_papers\121133-Mechanical strain induces expression of C-reactive protien in Human blood vessels.pdf | |||||
| 13 | mechanical testing vessels | Wang et al. | 2020 | artificial small diameter blood vessels: materials, fabrication, surface modification, mehcanical properties and bioactive functionalities | review paper regarding the generation of small diameter vessel grafts | youngs modulus: strain/stress: tissue stiffness. Nonlinear elasticity: radial expansion which is a low circumferential youngs modulus (20-50 kPa). Compliance measurements: is the chnage of the tube that occirs in response to pressure variations within the lumen. this could lead to damage in the endothelial layer. Burst pressure measurements: is the maximum pressure that a vascular vessel can withstand before an accute leak occurs and it fails. suture retention test: measures the adhesion of an implant to surrounding tissue. it is a kew issue for surgery as its resistance to the stresstes associated with implantation can only be established by determining the force necessary to pull a suture through a wall. | ..\08.Full_papers\121134-Artificial Small diameter Blood vessels- Materials, Fabrications, Surface modification, mechanical properties, bioactive functionalities.pdf | |||||||||||||
| 14 | mechanical testing vessels | solan et al. | 2009 | effects of mechanical stretch on collagen and cross linking in engineered blood vessels | cell culture of VSMC on a polymeric tube | ..\08.Full_papers\121135-effects of mechanical stretch on collagen and cross linking in engineered blood vessels.pdf | ||||||||||||||
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| number | Key words in search | Author(s) | Year | Title | Tissue type | clinical info | comment | Accepted? | link to paper | ||
| age | weight | flow rate | |||||||||
| 1 | flow rate piglet carotid | Olver et al. | 2016 | carotid artery vascular mechanics serve as biomarkers of cognitive dysfunction in aortic-banded miniature swine that can be treated with an exercise intervention | yucatan pig's carotid artery | 8 months | n/a | 400 ml/min | yes | ..\08.Full_papers\121117-Carotid artery vascular mechanics serve as biomarkers of cognitive dysfunction in aortic-banded minature swine that can be treated with an excercise intervention.pdf | |
| 2 | pig carotid artery | marcel et al. | 1995 | carotid blood flow distribution, haemodynamics and inotropic responses following calcitonin gene-realted peptide in the pig | yorkshire pig | n/a | 15-17 kg | 50 ml/min | blood flow was increased from baseline | no | ..\08.Full_papers\121118-carotid blood flow distribution, haemodynamics and inotropic responses following calcitonin gene related peptide in the pig.pdf |
| 3 | pig carotid artery | Ishii et al. | 2005 | Swine Model of carotid artery atherosclerosis: experimental induction by surgical partial ligation and dietary hypercholesterolemia | yucatan mini pigs | 6 months | 20-30 kg | n/a | good explanation of funciton of endothelial layer | no | ..\08.Full_papers\121119-swine model of carotid artery atherosclerosis experimental induction by surgical partial ligation and dietary hypercholesterolemia.pdf |
| 4 | pig carotid artery | Mangla et al. | 2015 | Endovascular external carotid artery occlusion for brain selective targeting: a cerebrovascular swine model | yorkshire swine | 18-36 weeks | 35-55 kg | n/a | flow rate in brain (not in carotid!) | no | ..\08.Full_papers\121120-endovascular external carotid artery occlusion for brain selective targeting a cerebrovascular swine model.pdf |
| 5 | pig carotid artery | Saez et al. | 2016 | Microstructural quantification of collagen fiber orientations and ist integration in constitutive modeling of porcine carotid artery | porcine common carotid arteries | n/a | n/a | n/a | paper is useful for mechanical testing ! | no | ..\08.Full_papers\121121-Microstructural quantificatin of collagen fiber orientations and its integration in constitutive modeling of the porcine carotid artery.pdf |
| 6 | pig carotid artery blood flow | Mrowczynski et al. | 2014 | porcine carotid artery replacement with biodegradable electrospun poly-e-caprolactone vascular prosthesis | land race pigs | n/a | 30-45 kg | 260 ml/min | yes | ..\08.Full_papers\121122-Porcine carotid artery replacement with biodegradable electrospun poly-e-caprolactone vascular prosthesis.pdf | |
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| Running number | Key words in search | Author(s) | Year | Title | Tissue type | Method | DNAse concentration | DNAse time | MgCl2 | Results | Mechanism | Benefits | Disadvantages | Link to paper | ||
| stat. Perf. | decell time | concentration | ||||||||||||||
| 1 | dnase decellularization | McCrary et al. | 2020 | novel sodium deoxycholate based chemical decellularization method for peripheral nerve | nerves | perf | over 3 days | 3% SDC | 75U/mL | 3 hr | ..\08.Full_papers\121136-novel sodium deoxycholate based chemical decellularization method for peripheral nerve.pdf | |||||
| 2 | dnase decellularization | Ahearne,M. | 2020 | corneal extracellular matrix decellularization | pig corneas | perf | over 5 dyas | 0.5% SDS+5%tritonx-100 | 10U/mL +(10U/mL Rnase) | 1 hr at 37C | ..\08.Full_papers\121137-corneal extracellular matrix decellularization.pdf | |||||
| 3 | dnase decellularization | Yamanaka et al. | 2020 | decellularization of submillimeter-diameter vascular scaffolds using peracetic acid | rat tail arteries | perf | over 3 days | 0.1% PAA +4%EtOH | 40U/mL | 3 days at 37C | 20 mM | ..\08.Full_papers\121138-decellularization of submillimeter-diameter vascular scaffolds using peracetic acid.pdf | ||||
