2022-07-18_ Experimental data- Drug injection and Pharmacokinetics_Stea pk

Selected Cell

Cell:

Value:

Drug Calculator Feeding Plan documentation Blood sampling Operation table pf Exp. PK_Raw data



	Drug name	Commercial Drug used	Dose; Reference	Drug name	Concentration Stock solution (mg/1000µl)	Dose mg/ 1000g body weight	drug preparation for 100gr of mouse (gm)	needed volume of stock solution (µl)	concentration of drug in injected volume (mg/ml)	injection volume (µl)/g mouse/drug	total volume of drug solution for 100 gr of mouse	Added water (µl)	body weight of mouse (gr)	vol drug dilution per mouse (ml)	cocktail form of injection
i.p; Single injection in right side of the abdomen	Codeine	(Codeine.phosphate.hemihydrate)_(5mg/1000µl)	(Schenk, 2017, DOI: 10.1038/s41598-017-04574-z,	Codeine	5	2	100	40	1	2	200	160	30	60	180
	Caffeine	Citrate de cafeine cooper (Caffeine citrate 25 mg/1000µl)		Caffeine	25	5	100	20	2.5	2	200	180	30	60	180
	Midazolam	Midazolam B. Braun (5mg/1000µl)		Midazolam	5	2	100	40	1	2	200	160	30	60	180

								Total volume of drug solution for 100gr of mouse	600
					Total volume of cocktail		Entry nr.	Body weight of mouse (gr)	Total volume of cocktail (µl)/mouse	Truely injected volume (ml)	Calculated error (%)
					Date	Animal-ID	600

					2021-04-21T0:0:0+0000	MNT-011	1306	28.5	171	170	0.005847953216374269
					2021-04-21T0:0:0+0000	MNT-012	1309	28.5	171	170	0.005847953216374269
					2021-04-26T0:0:0+0000	MNT-047	1338	30.77	184.62	185	0.0020582818762864014
					2021-04-26T0:0:0+0000	MNT-048	1339	30.71	184.26	185	0.004016064257028162
					2021-04-30T0:0:0+0000	MNT-049	1340	26.75	160.5	160	0.003115264797507788
					2021-04-30T0:0:0+0000	MNT-050	1341	30.32	181.92	182.5	0.0031882145998241673
					2021-05-03T0:0:0+0000	MNT-053	1344	31.41	188.46	187.5	0.005093919134033789
					2021-05-03T0:0:0+0000	MNT-054	1345	31.38	188.28	187.5	0.004142766093052906
					2021-05-05T0:0:0+0000	MNT-055	1346	30.41	182.46	182.5	2.1922613175486157E-4
					2021-05-05T0:0:0+0000	MNT-056	1347	29.2	175.2	175	0.0011415525114154602
					2021-05-07T0:0:0+0000	MNT-057	1348	34.46	206.76	207.5	0.003579028825691667
					2021-05-07T0:0:0+0000	MNT-058	1349	29.19	175.14	175	7.993605115906496E-4
					2021-05-17T0:0:0+0000	MNT-059	1350	32.3	193.8	195	0.006191950464396226
					2021-05-17T0:0:0+0000	MNT-060	1351	32.75	196.5	197.5	0.005089058524173028
					2021-05-19T0:0:0+0000	MNT-061	1352	33.1	198.6	197.5	0.005538771399798562
					2021-05-19T0:0:0+0000	MNT-062	1353	34.37	206.22	205	0.005916012025991654
					2021-05-21T0:0:0+0000	MNT-063	1354	31.7	190.2	190	0.0010515247108306449
					2021-05-21T0:0:0+0000	MNT-064	1355	39.4	236.4	237.5	0.00465313028764803
					2021-05-26T0:0:0+0000	MNT-051	1342	29.8	178.8	180	0.00671140939597309
					2021-05-26T0:0:0+0000	MNT-052	1343	32.18	193.08	192.5	0.003003936192251981

	Normal food	Fatty food for 2wks		Fatty food for 4wks																																					druig injection start
				Fat food feeding for Stea Pk_MAA (19.04.2021-15.05.2021)										Change the food completely on Monday and Thursday
		Store fatty food in dark!								First, weigh the ramining food, then fill the food container up to (gm)												50	gm
				Wk-01
Serial Nr	Animal Exp. ID	Entry-number		Srain		Type of food
			Ear Pinch		Company		2021-04-19T0:0:0+0000			2021-04-20T0:0:0+0000			2021-04-21T0:0:0+0000			2021-04-22T0:0:0+0000			2021-04-23T0:0:0+0000			2021-04-24T0:0:0+0000			2021-04-25T0:0:0+0000			2021-04-26T0:0:0+0000			2021-04-27T0:0:0+0000			2021-04-28T0:0:0+0000			2021-04-29T0:0:0+0000			2021-04-30T0:0:0+0000			2021-05-01T0:0:0+0000			2021-05-02T0:0:0+0000			2021-05-03T0:0:0+0000			2021-05-04T0:0:0+0000			2021-05-05T0:0:0+0000			2021-05-06T0:0:0+0000			2021-05-07T0:0:0+0000			2021-05-08T0:0:0+0000			2021-05-09T0:0:0+0000			2021-05-10T0:0:0+0000			2021-05-11T0:0:0+0000			2021-05-12T0:0:0+0000			2021-05-13T0:0:0+0000			2021-05-14T0:0:0+0000			2021-05-15T0:0:0+0000			2021-05-16T0:0:0+0000			2021-05-17T0:0:0+0000			2021-05-18T0:0:0+0000			2021-05-19T0:0:0+0000			2021-05-20T0:0:0+0000			2021-05-21T0:0:0+0000			2021-05-22T0:0:0+0000			2021-05-23T0:0:0+0000			2021-05-24T0:0:0+0000			2021-05-25T0:0:0+0000			2021-05-26T0:0:0+0000
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1	MNT-047	1338	Ohne	C57BL/6J	Janvier	Normal food	30.4	25	x	31.24	21.25	3.75	28.83	21.65	3.3500000000000014	31.28	21.725	3.2749999999999986	30.48	21.275	3.7250000000000014	30.36	21.38	3.620000000000001	30.57	21.555	3.4450000000000003	30.77	22.28	2.719999999999999	Pk and sacrifice					50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50
2	MNT-048	1339	1Li	C57BL/6J	Janvier	Normal food	30.24	25	x	31.4	21.25	3.75	30.45	21.65	3.3500000000000014	30.37	21.725	3.2749999999999986	30.76	21.275	3.7250000000000014	30.46	21.38	3.620000000000001	31.57	21.555	3.4450000000000003	30.71	22.28	2.719999999999999						50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50
3	MNT-049	1340	Ohne	C57BL/6J	Janvier	Normal food	27.86	25	x	27.72	21.25	3.75	26.5	21.725	3.2749999999999986	27.02	21.325	3.6750000000000007	26.52	21.655	3.344999999999999	26.51	21.53	3.469999999999999	26.64	21.855	3.1449999999999996	27.46	20.98	4.02	27.57	21.45	3.5500000000000007	26.92	21.425	3.5749999999999993	26.92	22.31	2.6900000000000013	26.75	22.065	2.9349999999999987	Pk and sacrifice								50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50
4	MNT-050	1341	1Li	C57BL/6J	Janvier	Normal food	32.23	25	x	31.52	21.25	3.75	30.3	21.725	3.2749999999999986	31.58	21.325	3.6750000000000007	30.34	21.655	3.344999999999999	29.9	21.53	3.469999999999999	29.56	21.855	3.1449999999999996	30.24	20.98	4.02	30.81	21.45	3.5500000000000007	30.29	21.425	3.5749999999999993	29.98	22.31	2.6900000000000013	30.32	22.065	2.9349999999999987						50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50			50
5	MNT-051	1342	Ohne	C57BL/6J	Janvier	Normal food	31.18	25	x	31.35	21.25	3.75	30.8	21.77	3.2300000000000004	31.4	21.6	3.3999999999999986	30.91	21.17	3.8299999999999983	30.59	21.535	3.465	31.65	21.53	3.469999999999999	31.42	21.47	3.530000000000001	31.85	21.13	3.870000000000001	30.9	21.85	3.1499999999999986	31.25	22.005	2.995000000000001	31.23	22.045	2.9549999999999983	30.13	22.015	2.9849999999999994	31.53	22.02	2.9800000000000004	30.57	22.02	2.9800000000000004	30.76	22.05	2.9499999999999993	30.93	21.78	3.219999999999999	31.02	21.985	3.0150000000000006	30.42	22.005	2.995000000000001	30.18	21.77	3.2300000000000004	29.69	21.67	3.3299999999999983	30.82	21.3	3.6999999999999993	31.34	22.12	2.879999999999999	30.85	21.5	3.5	30.64	21.8	3.1999999999999993	30	22.235	2.7650000000000006	30.28	22.005	2.995000000000001	29.47	21.915	3.085000000000001	30.07	21.875	3.125	30.16	21.86	3.1400000000000006	30.41	21.905	3.094999999999999	30.3	21.94	3.0599999999999987	30.32	22.37	2.629999999999999	29.69	22.855	2.1449999999999996	30	20.74	4.260000000000002	30	23.325	1.6750000000000007	31	21.86	3.1400000000000006	29.8	22.08	2.9200000000000017
6	MNT-052	1343	1Li	C57BL/6J	Janvier	Normal food	32.78	25	x	32.49	21.25	3.75	32.05	21.77	3.2300000000000004	32.42	21.6	3.3999999999999986	32.9	21.17	3.8299999999999983	31.52	21.535	3.465	32.47	21.53	3.469999999999999	32.02	21.47	3.530000000000001	32.91	21.13	3.870000000000001	32.09	21.85	3.1499999999999986	32.27	22.005	2.995000000000001	31.98	22.045	2.9549999999999983	32	22.015	2.9849999999999994	32.74	22.02	2.9800000000000004	31.97	22.02	2.9800000000000004	32.85	22.05	2.9499999999999993	33.1	21.78	3.219999999999999	32.39	21.985	3.0150000000000006	32.38	22.005	2.995000000000001	32.11	21.77	3.2300000000000004	32.35	21.67	3.3299999999999983	33.22	21.3	3.6999999999999993	33.04	22.12	2.879999999999999	33.15	21.5	3.5	32.22	21.8	3.1999999999999993	32.6	22.235	2.7650000000000006	32.6	22.005	2.995000000000001	31.79	21.915	3.085000000000001	32.1	21.875	3.125	32.53	21.86	3.1400000000000006	32.1	21.905	3.094999999999999	32.47	21.94	3.0599999999999987	32.47	22.37	2.629999999999999	31.9	22.855	2.1449999999999996	32	20.74	4.260000000000002	32	23.325	1.6750000000000007	32	21.86	3.1400000000000006	32.18	22.08	2.9200000000000017
7	MNT-053	1344	Ohne	C57BL/6J	Janvier	2Wks MCD+HFD	30.8	25	x	31.03	20.835	4.164999999999999	30.6	21.525	3.4750000000000014	30.99	21.45	3.5500000000000007	31.53	20.75	4.25	31.34	21.17	3.8299999999999983	31.55	21.33	3.6700000000000017	31.57	21.665	3.335000000000001	31.93	21.015	3.9849999999999994	31.47	21.845	3.155000000000001	31.7	21.835	3.164999999999999	32.16	19.865	5.135000000000002	31.93	21.375	3.625	31.97	22.29	2.710000000000001	31.41	21.73	3.2699999999999996	PK and sacrifice
8	MNT-054	1345	1Li	C57BL/6J	Janvier	2Wks MCD+HFD	29.96	25	x	30.67	20.835	4.164999999999999	30.37	21.525	3.4750000000000014	30.49	21.45	3.5500000000000007	30.7	20.75	4.25	30.82	21.17	3.8299999999999983	31.02	21.33	3.6700000000000017	31.16	21.665	3.335000000000001	31.06	21.015	3.9849999999999994	30.97	21.845	3.155000000000001	31.19	21.835	3.164999999999999	31.23	19.865	5.135000000000002	30.88	21.375	3.625	31.19	22.29	2.710000000000001	31.38	21.73	3.2699999999999996
9	MNT-055	1346	1Li	C57BL/6J	Janvier	2Wks MCD+HFD	30.75	25	x	31	21.225	3.7749999999999986	37.39	21.1	3.8999999999999986	32.45	20.465	4.535	32.26	20.87	4.129999999999999	30.57	20.81	4.190000000000001	31.02	20.975	4.024999999999999	30.37	21.48	3.5199999999999996	31.04	20.93	4.07	30.42	21.76	3.2399999999999984	30.42	21.79	3.210000000000001	30.37	20.84	4.16	30.6	20.88	4.120000000000001	30.61	22.1	2.8999999999999986	30.55	21.28	3.719999999999999	30.66	21.08	3.9200000000000017	30.14	21.6	3.3999999999999986	PK and sacrifice
10	MNT-056	1347	Ohne	C57BL/6J	Janvier	2Wks MCD+HFD	28.45	25	x	28.9	21.225	3.7749999999999986	28.85	21.1	3.8999999999999986	29.15	20.465	4.535	29.2	20.87	4.129999999999999	28.86	20.81	4.190000000000001	28.62	20.975	4.024999999999999	28.55	21.48	3.5199999999999996	28.89	20.93	4.07	28.62	21.76	3.2399999999999984	29.12	21.79	3.210000000000001	29.13	20.84	4.16	29.71	20.88	4.120000000000001	29.07	22.1	2.8999999999999986	28.93	21.28	3.719999999999999	29.26	21.08	3.9200000000000017	29.2	21.6	3.3999999999999986
11	MNT-057	1348	Ohne	C57BL/6J	Janvier	2Wks MCD+HFD	29.73	25	x	29.8	21.365	3.6350000000000016	29.942	20.67	4.329999999999998	29.61	20.97	4.030000000000001	29.48	21.365	3.6350000000000016	30.06	19.595	5.405000000000001	30.62	21.185	3.8150000000000013	30.91	21.335	3.664999999999999	31.22	19.49	5.510000000000002	31.49	20.9	4.100000000000001	31.65	21.49	3.5100000000000016	32.6	19.49	5.510000000000002	32.76	20.48	4.52	32.74	21.92	3.0799999999999983	32.92	21.27	3.7300000000000004	33.96	20.62	4.379999999999999	33.51	21.82	3.1799999999999997	33.62	22.42	2.5799999999999983	34.46	20.72	4.280000000000001	PK and sacrifice
12	MNT-058	1349	1li	C57BL/6J	Janvier	2Wks MCD+HFD	32.37	25	x	33.91	21.365	3.6350000000000016	35.45	20.67	4.329999999999998	36.82	20.97	4.030000000000001	34.65	21.365	3.6350000000000016	33.94	19.595	5.405000000000001	33.36	21.185	3.8150000000000013	32.49	21.335	3.664999999999999	32.4	20.35	4.649999999999999	31.12	21.45	3.5500000000000007	31.18	21.23	3.7699999999999996	30.64	21.21	3.789999999999999	30.35	21.22	3.780000000000001	30.06	21.56	3.4400000000000013	29.5	21	4	29.58	20.25	4.75	29.5	20.83	4.170000000000002	29.25	21.44	3.5599999999999987	29.19	19.72	5.280000000000001
13	MNT-059	1350	Ohne	C57BL/6J	Janvier	4Wks MCD+HFD	30.49	25	x	31.91	20.27	4.73	31.03	21.575	3.4250000000000007	31.9	21.42	3.5799999999999983	31.87	21.135	3.8649999999999984	31.5	21.365	3.6350000000000016	31.58	21.845	3.155000000000001	31.33	22.235	2.7650000000000006	31.53	21.29	3.710000000000001	31.2	22.335	2.664999999999999	32.25	22.24	2.7600000000000016	32.44	21.26	3.7399999999999984	31.37	21.685	3.3150000000000013	31.51	22.32	2.6799999999999997	32.22	21.675	3.3249999999999993	32.22	20.99	4.010000000000002	32.14	21.955	3.0450000000000017	31.9	21.95	3.0500000000000007	32.56	20.98	4.02	32.49	21.73	3.2699999999999996	32.26	21.785	3.215	31.95	22.305	2.6950000000000003	32.82	20.78	4.219999999999999	32.65	21.845	3.155000000000001	32.93	21.805	3.1950000000000003	32.14	20.455	4.545000000000002	32.86	21.8	3.1999999999999993	32.52	21.95	3.0500000000000007	32.3	22.455	2.5450000000000017	PK and sacrifice
14	MNT-060	1351	1Li	C57BL/6J	Janvier	4Wks MCD+HFD	30.83	25	x	31.24	20.27	4.73	30.9	21.575	3.4250000000000007	32.06	21.42	3.5799999999999983	31.76	21.135	3.8649999999999984	31.9	21.365	3.6350000000000016	31.76	21.845	3.155000000000001	31.65	22.235	2.7650000000000006	31.74	21.29	3.710000000000001	31.51	22.335	2.664999999999999	31.94	22.24	2.7600000000000016	31.77	21.26	3.7399999999999984	32.21	21.685	3.3150000000000013	32.04	22.32	2.6799999999999997	32.49	21.675	3.3249999999999993	32.45	20.99	4.010000000000002	32.65	21.955	3.0450000000000017	32.65	21.95	3.0500000000000007	32.95	20.98	4.02	32.33	21.73	3.2699999999999996	32.63	21.785	3.215	32.56	22.305	2.6950000000000003	33.25	20.78	4.219999999999999	33.23	21.845	3.155000000000001	32.19	21.805	3.1950000000000003	33.65	20.455	4.545000000000002	33.27	21.8	3.1999999999999993	33	21.95	3.0500000000000007	32.75	22.455	2.5450000000000017
15	MNT-061	1352	Ohne	C57BL/6J	Janvier	4Wks MCD+HFD	29.95	25	x	30.85	21.53	3.469999999999999	30.4	21.59	3.41	30.96	20.44	4.559999999999999	30.93	20.91	4.09	30.4	21.25	3.75	31.09	21.835	3.164999999999999	31.35	21.51	3.4899999999999984	32.69	21.19	3.8099999999999987	31.05	22.17	2.8299999999999983	31.4	22.56	2.4400000000000013	31.42	21.725	3.2749999999999986	31.4	21.535	3.465	31.35	22.955	2.0450000000000017	31.21	22.125	2.875	31.78	21.63	3.370000000000001	31.32	22.425	2.5749999999999993	31.6	22.2	2.8000000000000007	31.76	21.38	3.620000000000001	31.63	21.92	3.0799999999999983	31.95	22.05	2.9499999999999993	31.74	21.985	3.0150000000000006	32.23	21.725	3.2749999999999986	32.37	22.21	2.789999999999999	32.32	21.51	3.4899999999999984	33.18	20.965	4.035	33.1	22.23	2.7699999999999996	32.8	21.54	3.460000000000001	31.56	22.375	2.625	33.21	21.235	3.7650000000000006	33.1	21.8	3.1999999999999993	PK and sacrifice
16	MNT-062	1353	1Li	C57BL/6J	Janvier	4Wks MCD+HFD	30.98	25	x	31.12	21.53	3.469999999999999	30.34	21.59	3.41	31.61	20.44	4.559999999999999	31.66	20.91	4.09	31.6	21.25	3.75	32.18	21.835	3.164999999999999	32.08	21.51	3.4899999999999984	32.91	21.19	3.8099999999999987	32.33	22.17	2.8299999999999983	32.45	22.56	2.4400000000000013	32.63	21.725	3.2749999999999986	32.82	21.535	3.465	32.42	22.955	2.0450000000000017	32.35	22.125	2.875	32.6	21.63	3.370000000000001	32.41	22.425	2.5749999999999993	32.45	22.2	2.8000000000000007	32.8	21.38	3.620000000000001	32.6	21.92	3.0799999999999983	32.25	22.05	2.9499999999999993	32.59	21.985	3.0150000000000006	32.95	21.725	3.2749999999999986	32.43	22.21	2.789999999999999	32.73	21.51	3.4899999999999984	33.52	20.965	4.035	33.42	22.23	2.7699999999999996	33.83	21.54	3.460000000000001	33.53	22.375	2.625	34.12	21.235	3.7650000000000006	34.37	21.8	3.1999999999999993
17	MNT-063	1354	Ohne	C57BL/6J	Janvier	4Wks MCD+HFD	26.1	25	x	26.2	21.965	3.035	26.03	21.39	3.6099999999999994	26.75	21.14	3.8599999999999994	26.69	20.965	4.035	27.59	19.5	5.5	28.01	21.19	3.8099999999999987	27.76	21.175	3.8249999999999993	27.65	20.645	4.355	28.37	21.045	3.9549999999999983	28.54	21.865	3.1350000000000016	28.43	20.98	4.02	28.15	21.315	3.6849999999999987	28.17	22.29	2.710000000000001	28.67	21.08	3.9200000000000017	29.04	21.565	3.4349999999999987	28.79	22.11	2.8900000000000006	28.8	21.91	3.09	29.17	20.81	4.190000000000001	29.7	21.11	3.8900000000000006	29.03	22.085	2.914999999999999	29.22	21.78	3.219999999999999	29.72	20.785	4.215	30.16	21.165	3.835000000000001	29.78	21.595	3.405000000000001	30.24	20.865	4.135000000000002	30.65	21.15	3.8500000000000014	31.25	19.115	5.885000000000002	30.58	22.685	2.3150000000000013	30.86	21.515	3.4849999999999994	30.71	22.095	2.905000000000001	30.59	21.825	3.1750000000000007	31.7	20.54	4.460000000000001	PK and sacrifice
18	MNT-064	1355	1Li	C57BL/6J	Janvier	4Wks MCD+HFD	31.35	25	x	31.65	21.965	3.035	31.69	21.39	3.6099999999999994	32.56	21.14	3.8599999999999994	32.65	20.965	4.035	34.23	19.5	5.5	34.8	21.19	3.8099999999999987	34.65	21.175	3.8249999999999993	35.25	20.645	4.355	35.41	21.045	3.9549999999999983	35.62	21.865	3.1350000000000016	36.18	20.98	4.02	36	21.315	3.6849999999999987	26.35	22.29	2.710000000000001	36.65	21.08	3.9200000000000017	36.89	21.565	3.4349999999999987	36.53	22.11	2.8900000000000006	36.3	21.91	3.09	36.33	20.81	4.190000000000001	36.79	21.11	3.8900000000000006	36.68	22.085	2.914999999999999	36.65	21.78	3.219999999999999	37.28	20.785	4.215	37.38	21.165	3.835000000000001	37.24	21.595	3.405000000000001	38.11	20.865	4.135000000000002	37.67	21.15	3.8500000000000014	39.38	19.115	5.885000000000002	38.01	22.685	2.3150000000000013	37.94	21.515	3.4849999999999994	37.22	22.095	2.905000000000001	38.02	21.825	3.1750000000000007	39.4	20.54	4.460000000000001

100

Run

Date

Species

Animal ID

Entry no

Treatmnt

Tube

Date of birth-year (w)

used before

Protocol number

Age (month)

Weight (gr)

Restrainer

blood volume (µl)

comments_Filling level/Problems

Time point

Day time of sampling

Scoring result after last blood sample

2021-04-21T0:0:0+0000

Mouse

Bl6

MNT-011

1306

Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg)

Midazolam

2020-07-08T0:0:0+0000

BY NEH (Midazolam injection)

UKJ-19-020

9.566666666666666

28.5

Yes

9µl

Full

00min

1899-12-31T13:0:0+0000

9µl

Full

05min

1899-12-31T15:50:0+0000

9µl

Full

10min

1899-12-31T15:55:0+0000

9µl

Full

15min

1899-12-31T16:0:0+0000

9µl

Full

30min

1899-12-31T16:15:0+0000

9µl

Full

01hr

1899-12-31T16:45:0+0000

9µl

Full

02hr

1899-12-31T17:45:0+0000

9µl

Full

04hr

1899-12-31T19:45:0+0000

9µl

Full

24hr

1900-01-01T15:45:0+0000

2021-04-21T0:0:0+0000

Mouse

Bl6

MNT-011

1306

Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg)

Caffeine

2020-07-08T0:0:0+0000

BY NEH (Midazolam injection)

UKJ-19-020

9.566666666666666

28.5

Yes

9µl

Full

00min

1899-12-31T13:0:0+0000

9µl-0,5µl

Full except 0,5mm

05min

1899-12-31T15:50:0+0000

9µl

Full

10min

1899-12-31T15:55:0+0000

9µl

Full

15min

1899-12-31T16:0:0+0000

9µl

Full

30min

1899-12-31T16:15:0+0000

9µl

Full

01hr

1899-12-31T16:45:0+0000

9µl

Full

02hr

1899-12-31T17:45:0+0000

9µl

Full

04hr

1899-12-31T19:45:0+0000

9µl

Full

24hr

1900-01-01T15:45:0+0000

2021-04-21T0:0:0+0000

Mouse

Bl6

MNT-011

1306

Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg)

Codeine

2020-07-08T0:0:0+0000

BY NEH (Midazolam injection)

UKJ-19-020

9.566666666666666

28.5

Yes

9µl

Full

00min

1899-12-31T13:0:0+0000

9µl

Full

05min

1899-12-31T15:50:0+0000

9µl

Full

10min

1899-12-31T15:55:0+0000

9µl

Full

15min

1899-12-31T16:0:0+0000

9µl

Full

30min

1899-12-31T16:15:0+0000

9µl

Full

01hr

1899-12-31T16:45:0+0000

9µl

Full

02hr

1899-12-31T17:45:0+0000

9µl

Full

04hr

1899-12-31T19:45:0+0000

9µl

Full

24hr

1900-01-01T15:45:0+0000

2021-04-21T0:0:0+0000

Mouse

Bl6

MNT-014

1309

Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg)

Midazolam

2020-07-08T0:0:0+0000

BY NEH (Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg) injection)

UKJ-19-020

9.566666666666666

28.5

Yes

9µl-2mm

Fulle except 2mm

00min

1899-12-31T13:0:0+0000

9µl

Full

05min

1899-12-31T15:50:0+0000

9µl

Full

10min

1899-12-31T15:55:0+0000

9µl

Full

15min

1899-12-31T16:0:0+0000

9µl

Full

30min

1899-12-31T16:15:0+0000

9µl

Full

01hr

1899-12-31T16:45:0+0000

9µl

Full

02hr

1899-12-31T17:45:0+0000

9µl

Full

04hr

1899-12-31T19:45:0+0000

9µl

Full

24hr

1900-01-01T15:45:0+0000

2021-04-21T0:0:0+0000

Mouse

Bl6

MNT-014

1309

Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg)

Caffeine

2020-07-08T0:0:0+0000

BY NEH (Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg) injection)

UKJ-19-020

9.566666666666666

28.5

Yes

9µl

Full

00min

1899-12-31T13:0:0+0000

9µl

Full

05min

1899-12-31T15:50:0+0000

9µl-2mm

Full except 2mm

10min

1899-12-31T15:55:0+0000

9µl

Full

15min

1899-12-31T16:0:0+0000

9µl

Full

30min

1899-12-31T16:15:0+0000

9µl

Full _low Hematocrit

01hr

1899-12-31T16:45:0+0000

9µl

Full

02hr

1899-12-31T17:45:0+0000

9µl

Full

04hr

1899-12-31T19:45:0+0000

9µl

Full

24hr

1900-01-01T15:45:0+0000

2021-04-21T0:0:0+0000

Mouse

Bl6

MNT-014

1309

Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg)

Codeine

2020-07-08T0:0:0+0000

BY NEH (Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg) injection)

UKJ-19-020

9.566666666666666

28.5

Yes

9µl

Full

00min

1899-12-31T13:0:0+0000

9µl

Full

05min

1899-12-31T15:50:0+0000

9µl

Full

10min

1899-12-31T15:55:0+0000

9µl

Full

15min

1899-12-31T16:0:0+0000

9µl

Full

30min

1899-12-31T16:15:0+0000

9µl

Full

01hr

1899-12-31T16:45:0+0000

9µl

Full

02hr

1899-12-31T17:45:0+0000

9µl

Full

04hr

1899-12-31T19:45:0+0000

9µl

Full

24hr

1900-01-01T15:45:0+0000

Run

Date

Species

Animal ID

Entry no

Treatmnt

Tube

Date of birth-year (w)

used before

Protocol number

Age (month)

Weight (gr)

Restrainer

blood volume (µl)

comments_Filling level/Problems

Time point

Day time of sampling

Scoring result after last blood sample

2021-04-26T0:0:0+0000

Mouse

Bl6

MNT-047

1338

Control+Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg)

Midazolam

2020-08-31T0:0:0+0000

UKJ-19-020

7.966666666666667

31.77

Yes

2samples (9µl-3mm&9-4mm)

Fulle except 3mm

00min (2samples)

1899-12-31T7:0:0+0000

9µl

Full

05min

1899-12-31T8:40:0+0000

9µl

Full

10min

1899-12-31T8:45:0+0000

9µl-0,25mm

Full_Very small gas bubble (0,25mm)

15min

1899-12-31T8:50:0+0000

9µl

Full

30min

1899-12-31T9:5:0+0000

9µl

Full

01hr

1899-12-31T9:35:0+0000

9µl

Full

02hr

1899-12-31T10:35:0+0000

9µl

Full

04hr

1899-12-31T12:35:0+0000

9µl

Full

12hr

1899-12-31T20:35:0+0000

9µl

Full

24hr

1899-12-31T8:35:0+0000

2021-04-26T0:0:0+0000

Mouse

Bl6

MNT-047

1338

Control+Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg)

Caffeine

2020-08-31T0:0:0+0000

UKJ-19-020

7.966666666666667

31.77

Yes

9µl

Full

00min

1899-12-31T7:0:0+0000

9µl

Full

05min

1899-12-31T8:40:0+0000

9µl

Full

10min

1899-12-31T8:45:0+0000

9µl

Full

15min

1899-12-31T8:50:0+0000

9µl

Full

30min

1899-12-31T9:5:0+0000

9µl

Full

01hr

1899-12-31T9:35:0+0000

9µl

Full

02hr

1899-12-31T10:35:0+0000

9µl

Full

04hr

1899-12-31T12:35:0+0000

9µl

Full

12hr

1899-12-31T20:35:0+0000

9µl

Full

24hr

1899-12-31T8:35:0+0000

2021-04-26T0:0:0+0000

Mouse

Bl6

MNT-047

1338

Control+Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg)

Codeine

2020-08-31T0:0:0+0000

UKJ-19-020

7.966666666666667

31.77

Yes

9µl

Full

00min

1899-12-31T7:0:0+0000

9µl

Full

05min

1899-12-31T8:40:0+0000

9µl

Full

10min

1899-12-31T8:45:0+0000

9µl

Full

15min

1899-12-31T8:50:0+0000

9µl

Full

30min

1899-12-31T9:5:0+0000

9µl

Full

01hr

1899-12-31T9:35:0+0000

9µl

Full

02hr

1899-12-31T10:35:0+0000

9µl

Full

04hr

1899-12-31T12:35:0+0000

9µl

Full

12hr

1899-12-31T20:35:0+0000

9µl

Full

24hr

1899-12-31T8:35:0+0000

Run

Date

Species

Animal ID

Entry no

Treatmnt

Tube

Date of birth-year (w)

used before

Protocol number

Age (month)

Weight (gr)

Restrainer

blood volume (µl)

comments_Filling level/Problems

Time point

Day time of sampling

Scoring result after last blood sample

2021-04-26T0:0:0+0000

Mouse

Bl6

MNT-048

1339

Control+Drug coktails (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg)

Midazolam

2020-08-31T0:0:0+0000

UKJ-19-020

7.966666666666667

31.71

Yes

9µl

Full

00min (2samples)

1899-12-31T7:0:0+0000

9µl

Full

05min

1899-12-31T8:40:0+0000

9µl

Full

10min

1899-12-31T8:45:0+0000

9µl

Full

15min

1899-12-31T8:50:0+0000

9µl

Full

30min

1899-12-31T9:5:0+0000

9µl

Full

01hr

1899-12-31T9:35:0+0000

9µl

Full

02hr

1899-12-31T10:35:0+0000

9µl

Full

04hr

1899-12-31T12:35:0+0000

9µl

Full

12hr

1899-12-31T20:35:0+0000

Animal were assigned under group B in the animal application

Serial Nr

Animal EXP. ID

Entry-number

Ear pinch

Srain

Date of birth

Treatment

Comment to animal

Drugs

Animal weight at the day of drug injection (gm)

Animal weight at day of sacrifice (gm)

Day of drug cocktails injection

Other treatment

Day of sacrifice

Age months

Operation

MNT-047

1338

Ohne

C57BL/6J

2020-08-31T0:0:0+0000

Normal food

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

30.71

28.5

2021-04-26T0:0:0+0000

0,0048 Buprenovet

2021-04-27T0:0:0+0000

7.966666666666667

Sacrifice

MNT-048

1339

1Li

C57BL/6J

2020-08-31T0:0:0+0000

Normal food

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

30.77

29.5

2021-04-26T0:0:0+0000

0,005 Buprenovet

2021-04-27T0:0:0+0000

7.966666666666667

Sacrifice

MNT-049

1340

Ohne

C57BL/6J

2020-08-31T0:0:0+0000

Normal food

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

26.75

2021-04-30T0:0:0+0000

0,004 Buprenovet

2021-05-01T0:0:0+0000

8.1

Sacrifice

MNT-050

1341

1Li

C57BL/6J

2020-08-31T0:0:0+0000

Normal food

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

30.32

2021-04-30T0:0:0+0000

0,0046 Buprenovet

2021-05-01T0:0:0+0000

8.1

Sacrifice

MNT-051

1342

Ohne

C57BL/6J

2020-08-31T0:0:0+0000

Normal food

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

29.8

2021-05-26T0:0:0+0000

0,0048 Buprenovet

2021-05-27T0:0:0+0000

8.966666666666667

Sacrifice

MNT-052

1343

1Li

C57BL/6J

2020-08-31T0:0:0+0000

Normal food

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

32.18

2021-05-26T0:0:0+0000

0,0054 Buprenovet

2021-05-27T0:0:0+0000

8.966666666666667

Sacrifice

MNT-053

1344

Ohne

C57BL/6J

2020-08-31T0:0:0+0000

2Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

31.41

2021-05-03T0:0:0+0000

0,005 Buprenovet

2021-05-04T0:0:0+0000

8.2

Sacrifice

MNT-054

1345

1Li

C57BL/6J

2020-08-31T0:0:0+0000

2Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

31.38

2021-05-03T0:0:0+0000

0,005 Buprenovet

2021-05-04T0:0:0+0000

8.2

Sacrifice

MNT-055

1346

1Li

C57BL/6J

2020-08-31T0:0:0+0000

2Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

30.41

2021-06-05T0:0:0+0000

0,005 Buprenovet

2021-05-06T0:0:0+0000

8.266666666666667

Sacrifice

MNT-056

1347

Ohne

C57BL/6J

2020-08-31T0:0:0+0000

2Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

29.2

2021-06-05T0:0:0+0000

0,0046 Buprenovet

2021-05-06T0:0:0+0000

8.266666666666667

Sacrifice

MNT-057

1348

Ohne

C57BL/6J

2020-08-31T0:0:0+0000

2Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

34.46

2021-05-07T0:0:0+0000

0,0048 Buprenovet

2021-05-08T0:0:0+0000

8.333333333333334

Sacrifice

MNT-058

1349

1li

C57BL/6J

2020-08-31T0:0:0+0000

2Wks MCD+HFD

Animal had skin problems, injuries

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

29.19

2021-05-07T0:0:0+0000

0,006 Buprenovet

2021-05-08T0:0:0+0000

8.333333333333334

Sacrifice

MNT-059

1350

Ohne

C57BL/6J

2020-08-31T0:0:0+0000

4Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

32.3

2021-05-17T0:0:0+0000

0,0054 Buprenovet

2021-05-18T0:0:0+0000

8.666666666666666

Sacrifice

MNT-060

1351

1Li

C57BL/6J

2020-08-31T0:0:0+0000

4Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

32.75

32.5

2021-05-17T0:0:0+0000

0,0055 Buprenovet

2021-05-18T0:0:0+0000

8.666666666666666

Sacrifice

MNT-061

1352

Ohne

C57BL/6J

2020-08-31T0:0:0+0000

4Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

33.1

2021-05-19T0:0:0+0000

0,0056 Buprenovet

2021-05-20T0:0:0+0000

8.733333333333333

Sacrifice

MNT-062

1353

1Li

C57BL/6J

2020-08-31T0:0:0+0000

4Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

34.7

32.5

2021-05-19T0:0:0+0000

0,0056 Buprenovet

2021-05-20T0:0:0+0000

8.733333333333333

Sacrifice

MNT-063

1354

Ohne

C57BL/6J

2020-08-31T0:0:0+0000

4Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

31.7

2021-05-21T0:0:0+0000

0,0052 Buprenovet

2021-05-22T0:0:0+0000

8.8

Sacrifice

MNT-064

1355

1Li

C57BL/6J

2020-08-31T0:0:0+0000

4Wks MCD+HFD

cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg

39.4

2021-05-21T0:0:0+0000

0,0068 Buprenovet

2021-05-22T0:0:0+0000

8.8

Sacrifice

100

Date	Animal entry number	Animal ID	Treatment	sampling time point	sample name in the shipment	filling level_After sampling_Jena Lba	filling level in cm	corresponding volume in µL	dilution factor	Mother drug concentration	Metabolite 1	Metabolite 2	Metabolite 3	Metabolite 4
2021-04-30T0:0:0+0000										Midazolam (ng/ml)	1-OH-Midazolam (ng/ml)
	1340	MNT-049	Control animals injected ip with drug cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg	0min	MNT-049_00min_Midazolam	Full	3.2	9	1	<0,1	<0,1
				10min	MNT-049_10min_Midazolam	Full	3.2	9	1	13.9	14.3
				30min	MNT-049_30min_Midazolam	Fullexcept small gas bubble (0,25mm)	3.2	9	1	23.6	51.2
				1hr	MNT-049_01hr_Midazolam	Full	3.2	9	1	4.7	14.6
				1.5hr	MNT-049_1.5hr_Midazolam	Full	3.2	9	1	3.3	13.1
				2hr	MNT-049_02hr_Midazolam	Full	3.2	9	1	2.3	9
				2.5hr	MNT-049_2.5r_Midazolam	Full	3.2	9	1	11.6	55.7
				3hr	MNT-049_03hr_Midazolam	Full	3.2	9	1	1.4	3.7
				4hr	MNT-049_04hr_Midazolam	Full	3.2	9	1	1.2	3.3
				6hr	MNT-049_06hr_Midazolam	Full	3.1	8.72	1.03	<0,1	8.6
										Caffeine (µg/ml)
	1340	MNT-049	Control animals injected ip with drug cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg	0min	MNT-049_00min_Caffeine	Full	3.2	9	1	<0.010
				10min	MNT-049_10min_Caffeine	Full	3.2	9	1	1.198
				30min	MNT-049_30min_Caffeine	Full	3.2	9	1	0.353
				1hr	MNT-049_01hr_Caffeine	Full	3.2	9	1	1.12
				1.5hr	MNT-049_1.5hr_Caffeine	Full	3.1	8.72	1.03	0.576
				2hr	MNT-049_02hr_Caffeine	Full	3.1	8.72	1.03	0.617
				2.5hr	MNT-049_2.5hr_Caffeine	Full	3.1	8.72	1.03	0.456
				3hr	MNT-049_03hr_Caffeine	Full	1.1	3.09	2.91	0.41
				4hr	MNT-049_04hr_Caffeine	Full	3.2	9	1	0.237
				6hr	MNT-049_06hr_Caffeine	Full	3.1	8.72	1.03	0.027
										Codeine (ng/ml)	Norcodeine (ng/ml)	Codeine-6-Glucuronide (ng/ml)	Morphine-3-Glucuronide (ng/ml)	Morphine (ng/ml)
	1340	MNT-049	Control animals injected ip with drug cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg	0min	MNT-049_00min_Codeine	Full	3.1	8.72	1.15	<0.1	<0.5	<0.5	<0.1	<0.1
				10min	MNT-049_10min_Codeine	Full	3.1	8.72	1.15	273	47	15	229	11
				30min	MNT-049_30min_Codeine	Full	3.2	9	1.11	187	37	32	320	6.3
				1hr	MNT-049_01hr_Codeine	Full	2.8	7.88	1.27	92	29	18	188	3
				1.5hr	MNT-049_1.5hr_Codeine	Full	3.2	9	1.11	45	19	9.7	102	1.2
				2hr	MNT-049_2hr_Codeine	Full	3.2	9	1.11	30	13	6	67	0.6
				2.5hr	MNT-049_2.5hr_Codeine	Full	3.1	8.72	1.15	21	8.9	4.2	45	0.2
				3hr	MNT-049_03hr_Codeine	Full	3.2	9	1.11	18	6.1	2.9	33	<0.1
				4hr	MNT-049_04hr_Codeine	Full	3.1	8.72	1.15	13	5	2.8	30	<0.1
				6hr	MNT-049_06hr_Codeine	Full except1mm	3.1	8.72	1.15	7.9	2.1	1.3	59	0.4

Date	Animal entry number	Animal ID	Treatment	sampling time point	sample name in the shipment	filling level_After sampling_Jena Lba	filling level in cm	corresponding volume in µL	dilution factor	Mother drug concentration	Metabolite 1	Metabolite 2	Metabolite 3	Metabolite 4
2021-04-30T0:0:0+0000										Midazolam (ng/ml)	1-OH-Midazolam (ng/ml)
	1341	MNT-050	Control animals injected ip with drug cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg	0min	MNT-049_00min_Midazolam	Full	3.2	9	1	<0,1	<0,1
				10min	MNT-049_10min_Midazolam	Full	3.2	9	1	11.2	14.1
				30min	MNT-049_30min_Midazolam	Full	3.2	9	1	116.5	271.8
				1hr	MNT-049_01hr_Midazolam	Full	3.2	9	1	26	96.6
				1.5hr	MNT-049_1.5hr_Midazolam	Full	3.2	9	1	7.5	23.1
				2hr	MNT-049_02hr_Midazolam	Full	3.2	9	1	2.1	5.6
				2.5hr	MNT-049_2.5r_Midazolam	Full	3.2	9	1	3.9	18.1
				3hr	MNT-049_03hr_Midazolam	Full	3.2	9	1	2.1	7.7
				4hr	MNT-049_04hr_Midazolam	Full	3.2	9	1	1.2	11.2
				6hr	MNT-049_06hr_Midazolam	Full	3.2	9	1	<0,1	2.1
										Caffeine (µg/ml)
	1341	MNT-050	Control animals injected ip with drug cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg	0min	MNT-049_00min_Caffeine	Full	3.1	8.72	1.03	<0.010
				10min	MNT-049_10min_Caffeine	Full	3.1	8.72	1.03	0.557
				30min	MNT-049_30min_Caffeine	Full	3.2	9	1	1.345
				1hr	MNT-049_01hr_Caffeine	Full	3.2	9	1	1.471
				1.5hr	MNT-049_1.5hr_Caffeine	Full	1.1	3.19	2.82	1.523
				2hr	MNT-049_02hr_Caffeine	Full	3.1	8.72	1.03	0.679
				2.5hr	MNT-049_2.5hr_Caffeine	Full	3.1	8.72	1.03	0.557
				3hr	MNT-049_03hr_Caffeine	Full	1.8	5.06	1.78	0.231
				4hr	MNT-049_04hr_Caffeine	Full	3.2	9	1	0.167
				6hr	MNT-049_06hr_Caffeine	Full	3.1	8.72	1.03	<0.010
										Codeine (ng/ml)	Norcodeine (ng/ml)	Codeine-6-Glucuronide (ng/ml)	Morphine-3-Glucuronide (ng/ml)	Morphine (ng/ml)
	1341	MNT-050	Control animals injected ip with drug cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg	0min	MNT-050_00min_Codeine	Full	3.1	8.72	1.15	<0.1	<0.5	<0.5	<0.1	<0.1
				10min	MNT-050_10min_Codeine	Full	3.1	8.72	1.15	225	47	11	163	10
				30min	MNT-050_30min_Codeine	Full	3.1	8.72	1.15	142	38	31	310	5.1
				1hr	MNT-050_01hr_Codeine	2 samples Sample 01 _Full except 9mm Sample 02_ Full except 10mm	1.7	4.78	2.09	128	51	34	379	6.7
				1.5hr	MNT-050_1.5hr_Codeine	Full	3	8.44	1.18	39	20	7	81	1.1
				2hr	MNT-050_2hr_Codeine	Full	3.2	9	1.11	35	18	6.8	79	1.3
				2.5hr	MNT-050_2.5hr_Codeine	Full	3.2	9	1.11	30	15	4.7	56	0.7
				3hr	MNT-050_03hr_Codeine	Full	2.9	8.16	1.23	24	13	4.1	51	0.7
				4hr	MNT-050_04hr_Codeine	Full	3.1	8.72	1.15	17	8.5	3.1	78	1.6
				6hr	MNT-050_06hr_Codeine	Full	3.1	8.72	1.15	11	3.1	0.6	65	1.6

Date	Animal entry number	Animal ID	Treatment	sampling time point	sample name in the shipment	filling level_After sampling_Jena Lba	filling level in cm	corresponding volume in µL	dilution factor	Mother drug concentration	Metabolite 1	Metabolite 2	Metabolite 3	Metabolite 4
2021-05-26T0:0:0+0000										Midazolam (ng/ml)	1-OH-Midazolam (ng/ml)
	1342	MNT-051	Control animals injected ip with drug cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg	0min	MNT-051_00min_Midazolam	Full	3.2	9	1	<0,1	<0,1
				10min	MNT-051_10min_Midazolam	Full	3.2	9	1	97.4	107.7
				30min	MNT-051_30min_Midazolam	Full	3.2	9	1	83	205.6
				1hr	MNT-051_01hr_Midazolam	Full	3.2	9	1	45.4	131.2
				1.5hr	MNT-051_1.5hr_Midazolam	Full	3.2	9	1	4	9.9
				2hr	MNT-051_02hr_Midazolam	Full	3.2	9	1	11.1	37.9
				2.5hr	MNT-051_2.5r_Midazolam	Full	3.2	9	1	2.6	9.7
				3hr	MNT-051_03hr_Midazolam	Full	3.2	9	1	8	51
				4hr	MNT-051_04hr_Midazolam	Full	3.2	9	1	3.7	33.4
				6hr	MNT-051_06hr_Midazolam	Full except 1mm	3.2	9	1	1.2	6.2
										Caffeine (µg/ml)
	1342	MNT-051	Control animals injected ip with drug cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg	0min	MNT-051_00min_Caffeine	Full	3.1	8.72	1.03	<0.010
				10min	MNT-051_10min_Caffeine	Full	3.2	9	1	0.839
				30min	MNT-051_30min_Caffeine	Full	3.2	9	1	1.2
				1hr	MNT-051_01hr_Caffeine	Full	3.1	8.72	1.03	1.148
				1.5hr	MNT-051_1.5hr_Caffeine	Full	3.1	8.72	1.03	0.587
				2hr	MNT-051_02hr_Caffeine	Full	3.2	9	1	0.671
				2.5hr	MNT-051_2.5hr_Caffeine	Full	3.1	8.72	1.03	0.555
				3hr	MNT-051_03hr_Caffeine	Full	3.1	8.72	1.03	0.479
				4hr	MNT-051_04hr_Caffeine	Full	3	9	1	0.219
				6hr	MNT-051_06hr_Caffeine	Full	2.9	8.16	1.1	0.037
										Codeine (ng/ml)	Norcodeine (ng/ml)	Codeine-6-Glucuronide (ng/ml)	Morphine-3-Glucuronide (ng/ml)	Morphine (ng/ml)
	1342	MNT-051	Control animals injected ip with drug cocktail consisting of (Midazolam 2mg/kg, Caffeine 5mg/kg, Codeine 2mg/kg	0min	MNT-051_00min_Codeine	Full	2.8	7.88	1.27	<0.1	<0.5	<0.5	<0.1	<0.1
				10min	MNT-051_10min_Codeine	Full	3.2	9	1.11	210	51	16	183	3.5
				30min	MNT-051_30min_Codeine	Full	3	8.44	1.18	178	51	25	224	3.8
				1hr	MNT-051_01hr_Codeine	Full	3.1	8.72	1.15	96	41	18	146	2.5
				1.5hr	MNT-051_1.5hr_Codeine	Full	2.9	8.16	1.23	67	34	10	98	1.6
				2hr	MNT-051_2hr_Codeine	Full	3.1	8.72	1.15	42	22	6.5	62	0.6